Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 389,925 (T)6→7 intergenic (‑198/‑326) hemB ← / → yaiT 5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA389,9191.T100.0% 932.3 / NA 247intergenic (‑192/‑332)hemB/yaiT5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (131/116);  total (131/116)

Only 100 of 282 total aligned reads displayed.

GAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACT  >  NC_000913_3_hsa_tpiA/389770‑390063
                                                                                                                                                      |                                                                                                                                                
      gggTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttt                                                                                                                                            >  4:53105/1‑150 (MQ=255)
        gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttttg                                                                                                                                          <  3:1227119/150‑3 (MQ=255)
          aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttttgga                                                                                                                                        >  2:1275106/1‑146 (MQ=255)
          aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttttgga                                                                                                                                        <  1:349254/150‑5 (MQ=255)
          aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttttgga                                                                                                                                        <  4:198001/150‑5 (MQ=255)
          aGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAtttttttgga                                                                                                                                        <  2:1628164/150‑5 (MQ=255)
            ggATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGt                                                                                                                                       >  4:496618/1‑150 (MQ=255)
              aTGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGttt                                                                                                                                     <  3:496618/150‑1 (MQ=255)
                gTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTgg                                                                                                                                   >  1:1455167/1‑150 (MQ=255)
                 ttttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGt                                                                                                                                  >  4:1125170/1‑150 (MQ=255)
                    tACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTgc                                                                                                                               >  2:153374/1‑150 (MQ=255)
                      cGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTgcgc                                                                                                                             >  2:1305972/1‑150 (MQ=255)
                       gAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgct                                                                                                                            >  3:270046/1‑150 (MQ=255)
                       gAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttttttt                                                                                                                                           >  4:1401559/1‑133 (MQ=255)
                        aaaGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctg                                                                                                                           <  2:2542090/150‑1 (MQ=255)
                          aGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctgct                                                                                                                         <  1:2241516/150‑1 (MQ=255)
                            ttGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTCATAGGTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCtat                                                                                                                       >  3:1154750/1‑150 (MQ=255)
                              gTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaa                                                                                                                     >  1:1414969/1‑150 (MQ=255)
                               tCGCGATGTTTACAAGAAGCGAATGGAAGAGAGGCTAGGACAACTTTTTAAGAGAAGCAGCAATAGTTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                    <  2:2235933/150‑1 (MQ=255)
                               tCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                    >  2:1033317/1‑150 (MQ=255)
                                 gcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAAtt                                                                                                                  >  1:2328166/1‑150 (MQ=255)
                                  cgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                                 <  1:1897492/150‑1 (MQ=255)
                                  cgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                                 <  2:1864983/150‑1 (MQ=255)
                                   gATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGa                                                                                                                <  4:243501/150‑1 (MQ=255)
                                    aTGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaa                                                                                                               <  2:410676/150‑1 (MQ=255)
                                      gTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                    >  1:2376676/1‑143 (MQ=255)
                                        tGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaataat                                                                                                           >  3:1385250/1‑150 (MQ=255)
                                             agaagaGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAAGAATTaaaa                                                                                                      >  2:2324393/1‑150 (MQ=255)
                                              gaagaGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATTTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAAc                                                                                                     >  2:374498/1‑150 (MQ=255)
                                               aagaGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACt                                                                                                    >  2:689476/1‑150 (MQ=255)
                                                 gagaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                                  <  1:2088455/150‑1 (MQ=255)
                                                 gagaATGGAAGAGAGGCCAGGAAATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                                  <  1:2538577/150‑1 (MQ=255)
                                                  agaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttg                                                                                                 >  3:1313180/1‑150 (MQ=255)
                                                    aaTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                               <  2:792373/150‑1 (MQ=255)
                                                    aaTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                               <  3:1176616/150‑1 (MQ=255)
                                                     aTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgttg                                                                                              >  2:1185832/1‑150 (MQ=255)
                                                      tGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGTGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgttgt                                                                                             >  2:46484/1‑149 (MQ=255)
                                                       ggAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCa                                                                                            >  2:2620205/1‑150 (MQ=255)
                                                         aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCata                                                                                          >  3:740580/1‑150 (MQ=255)
                                                         aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCata                                                                                          >  2:987836/1‑150 (MQ=255)
                                                          agagagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCatat                                                                                         <  3:22700/150‑1 (MQ=255)
                                                           gagagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgt                                                                                                <  1:320339/142‑1 (MQ=255)
                                                            agagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTc                                                                                       <  1:245210/150‑1 (MQ=255)
                                                             gagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCa                                                                                      <  2:724798/150‑1 (MQ=255)
                                                                gCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAg                                                                                   >  2:1121344/1‑150 (MQ=255)
                                                                    ggACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATaaa                                                                                                                    <  1:1991362/113‑1 (MQ=255)
                                                                    ggACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGata                                                                               >  1:27702/1‑150 (MQ=255)
                                                                       cATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATAttt                                                                            <  2:1455167/150‑1 (MQ=255)
                                                                        aTCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTa                                                                           >  1:925917/1‑150 (MQ=255)
                                                                          cTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                                    <  3:53846/91‑1 (MQ=255)
                                                                            ttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTg                                                                       >  4:747179/1‑150 (MQ=255)
                                                                            ttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATGTATCTg                                                                       >  1:1390703/1‑150 (MQ=255)
                                                                             tttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTgg                                                                      >  4:424226/1‑150 (MQ=255)
                                                                             tttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTgg                                                                      >  1:2614727/1‑150 (MQ=255)
                                                                             tttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTgg                                                                      <  1:1198034/150‑1 (MQ=255)
                                                                              ttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACt                                                                                                    <  1:1000897/119‑1 (MQ=255)
                                                                               tAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGatat                                                                              <  1:1226312/140‑1 (MQ=255)
                                                                               tAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtt                                                                    <  1:1305972/150‑1 (MQ=255)
                                                                                aaTAANAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCAATTGTTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATNATTAAAACTTTGTTGCATATTGATAGGATATTTATCTGgttt                                                                   <  1:46484/150‑1 (MQ=255)
                                                                                  tAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttttttt                                                                                                                                           >  4:53801/1‑74 (MQ=255)
                                                                                    gAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgt                                                               >  3:280333/1‑150 (MQ=255)
                                                                                      aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGCTATTTATCTGgtttgtgttt                                                             >  1:110097/1‑150 (MQ=255)
                                                                                      aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgttt                                                             <  2:762666/150‑1 (MQ=255)
                                                                                       aCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtttg                                                            <  2:2128764/150‑1 (MQ=255)
                                                                                       aCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtttg                                                            <  2:1220713/150‑1 (MQ=255)
                                                                                        cAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtttgt                                                           <  2:884907/150‑1 (MQ=255)
                                                                                          gCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGa                                                         <  4:1182952/150‑1 (MQ=255)
                                                                                            aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCa                                                                                      <  4:174288/119‑1 (MQ=255)
                                                                                            aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATa                                                                                    >  2:565157/1‑121 (MQ=255)
                                                                                            aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGata                                                       >  4:1360558/1‑150 (MQ=255)
                                                                                                atTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtattta                                                   <  1:161142/150‑1 (MQ=255)
                                                                                                 ttttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttat                                                  <  4:88903/150‑1 (MQ=255)
                                                                                                 ttttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttat                                                  <  1:574025/150‑1 (MQ=255)
                                                                                                  tttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttatt                                                 >  1:7108/1‑150 (MQ=255)
                                                                                                      tatTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                                             >  4:871126/1‑150 (MQ=255)
                                                                                                        ttCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGt                                           <  3:1523229/150‑1 (MQ=255)
                                                                                                        ttCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGt                                           <  4:1291640/150‑1 (MQ=255)
                                                                                                          cACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGatat                                                                              <  1:1723747/113‑1 (MQ=255)
                                                                                                          cACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGatat                                                                              >  2:1723747/1‑113 (MQ=255)
                                                                                                           aCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATg                                        >  1:99736/1‑150 (MQ=255)
                                                                                                            cTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTTCGCTGCTATAAATTGAATAATTAAAACTTTTTTGCATATTCATAGGATATTTTTCTGGTTTGTGTTTGTGATATTTATTATGCTTAttt                                       >  2:2481933/1‑148 (MQ=255)
                                                                                                             tGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCt                                      >  1:2117197/1‑150 (MQ=255)
                                                                                                               aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                               >  3:1635151/1‑91 (MQ=255)
                                                                                                               aaaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTc                                    >  2:1927564/1‑150 (MQ=255)
                                                                                                                aaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCa                                   <  3:53105/150‑1 (MQ=255)
                                                                                                                   tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaa                                >  1:67923/1‑150 (MQ=255)
                                                                                                                     tttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAAc                              >  3:109321/1‑150 (MQ=255)
                                                                                                                      ttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa                             >  1:405172/1‑150 (MQ=255)
                                                                                                                      ttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa                             >  1:1765676/1‑150 (MQ=255)
                                                                                                                       tttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTTATTTTTATTATGCTTATGCTTCAAAAACaa                            >  2:2394223/1‑150 (MQ=255)
                                                                                                                            tCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATa                                                                                    <  3:433935/89‑1 (MQ=255)
                                                                                                                              tttatttaTAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATc                     >  3:364166/1‑150 (MQ=255)
                                                                                                                               ttatttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCt                    <  1:374498/150‑1 (MQ=255)
                                                                                                                                tatttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTg                   <  1:537244/150‑1 (MQ=255)
                                                                                                                                         cTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGtt          <  2:325745/150‑1 (MQ=255)
                                                                                                                                         cTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGtt          <  3:871126/150‑1 (MQ=255)
                                                                                                                                          tGTTGGTTATTATTTTTTTTTTTTTTTGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTg         >  4:616245/1‑150 (MQ=255)
                                                                                                                                              ggTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaaa                               <  1:2508092/124‑1 (MQ=255)
                                                                                                                                                ttattatTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATAc   <  4:1593599/150‑1 (MQ=255)
                                                                                                                                                 tattatTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTAAAAAACAAAATTATCTGCAACATGTTGAAATACt  <  2:1414969/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GAAGCAGGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATACT  >  NC_000913_3_hsa_tpiA/389770‑390063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: