Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA3,946,1400GT34.2% 214.9 / 112.8 114noncoding (2437/2904 nt)rrlC23S ribosomal RNA of rrnC operon
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base T (21/18);  total (57/57)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 115 total aligned reads displayed.

AAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT  >  NC_000913_3_hsa_tpiA/3946079‑3946288
                                                             |                                                                                                                                                    
aaaGTAGGACTTAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGa                                                              <  4:687525/150‑1 (MQ=2)
aaaGTAGGACTTAGTGATCCGGCGGTCCCATGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGa                                                              <  2:2560301/150‑1 (MQ=2)
aaaGTAGGACTTAGGGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGa                                                              <  4:878137/150‑1 (MQ=2)
aaaGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGa                                                              >  2:1995081/1‑150 (MQ=11)
aaaGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGa                                                              >  3:1441785/1‑150 (MQ=11)
aaaGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGa                                                              <  1:2248175/150‑1 (MQ=11)
 aaGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAg                                                             <  1:263844/150‑1 (MQ=31)
    taggacttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTg                                                          >  4:570932/8‑150 (MQ=2)
    taggacttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTg                                                          >  1:1756151/8‑150 (MQ=2)
       gacttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTgtag                                                       >  3:1050236/5‑147 (MQ=2)
       gTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAg                                                       >  1:1426203/1‑150 (MQ=32)
        tCATAGTGGTCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGt                                                      <  1:772501/150‑1 (MQ=17)
        acttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGt                                                      <  1:501146/147‑1 (MQ=2)
         cttAGTGATCCGGCGGTCCCGTGTGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGtc                                                     >  3:480961/3‑149 (MQ=2)
          aTAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAg                                                    <  1:2399259/150‑1 (MQ=32)
             gTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTc                                                 <  2:308910/150‑1 (MQ=32)
              tGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGTCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTcc                                                <  2:1795401/150‑1 (MQ=17)
              tGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTcc                                                <  1:476396/150‑1 (MQ=32)
              tGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGCCGGCTCATCACATCCTGGAGCTGAAGTAGGTcc                                                <  4:53022/150‑1 (MQ=17)
               gATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCCCATCCTGGAGCTGAAGTAGGTccc                                               >  2:1880425/1‑150 (MQ=17)
               gATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTccc                                               >  2:2410579/1‑150 (MQ=32)
               gATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTccc                                               <  1:1950407/150‑1 (MQ=32)
                aTCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCa                                              >  2:1191456/1‑150 (MQ=32)
                     gtggtTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGt                                         >  4:264859/1‑150 (MQ=32)
                       ggtTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTGCTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTAt                                       <  2:1712344/150‑1 (MQ=17)
                       ggtCCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACTGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTAt                                       <  2:1858401/150‑1 (MQ=17)
                         ttCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg                                     <  3:1417632/150‑1 (MQ=32)
                         ttCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg                                     >  4:21133/1‑150 (MQ=32)
                         ttCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATgg                                     >  4:238663/1‑150 (MQ=32)
                           cTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCt                                   >  4:1162529/1‑150 (MQ=32)
                           cTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCt                                   >  2:1341876/1‑150 (MQ=32)
                            tGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTg                                  >  1:414369/1‑150 (MQ=32)
                            tGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTg                                  >  1:898225/1‑150 (MQ=32)
                            cgtgtGGAAGGGCCGTCGCTCAACGGCTAAAAGTTACCACGGGGATCACAGGTTTATCTCCTTAAAGTGCTCACACCGACGGCGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTg                                  <  1:909455/146‑1 (MQ=2)
                            cgtgtGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTg                                  >  2:1676127/5‑150 (MQ=2)
                             gAATGGAAGGGCCATCGCTCAACGGATAAGAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGt                                 >  3:1074314/1‑150 (MQ=17)
                             gAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGt                                 >  2:537933/1‑150 (MQ=32)
                             gAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGt                                 >  1:892638/1‑150 (MQ=32)
                             gAATGGAAGGGCCATCACTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGt                                 >  3:1298354/1‑150 (MQ=17)
                               gtGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTc                               <  3:1520276/149‑1 (MQ=2)
                               aTGGAAGCGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTc                               <  2:1370199/150‑1 (MQ=17)
                                tGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGa                                                              >  4:145518/1‑118 (MQ=2)
                                tGGAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGa                                                              <  3:145518/118‑1 (MQ=2)
                                 ggAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGc                             <  3:1085300/150‑1 (MQ=2)
                                  gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGATGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                            <  1:1603864/150‑1 (MQ=2)
                                  gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                            >  3:567947/1‑150 (MQ=2)
                                  gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                            <  4:687062/150‑1 (MQ=2)
                                  gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                            >  2:741873/1‑150 (MQ=2)
                                  gAAGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGcc                            <  3:1204093/150‑1 (MQ=2)
                                  gAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGcc                            >  3:1219692/1‑150 (MQ=32)
                                   aaGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACAACCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCa                           >  1:2216906/1‑150 (MQ=17)
                                   aaGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTGCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCa                           <  2:59960/150‑1 (MQ=17)
                                    aGGGCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGccca                          >  2:2537698/1‑148 (MQ=2)
                                     gggCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAtt                         >  2:1273442/1‑150 (MQ=32)
                                     gggCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAtt                         <  4:1234021/150‑1 (MQ=32)
                                     gggCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAtt                         <  2:161217/150‑1 (MQ=32)
                                      ggCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTc                               <  4:337250/143‑1 (MQ=2)
                                      ggCCGTCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTc                               >  3:337250/1‑143 (MQ=2)
                                      ggCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCAttt                        <  3:1162529/150‑1 (MQ=32)
                                        ccATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTaa                      >  1:122951/1‑150 (MQ=32)
                                          gtCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAg                    >  1:226657/2‑150 (MQ=2)
                                          gtCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAg                    >  1:2061646/2‑150 (MQ=11)
                                          aTCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAg                    <  2:2166540/150‑1 (MQ=32)
                                          aTCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAg                    >  2:1075882/1‑150 (MQ=32)
                                          aTCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCAGCTCATCACATCCTGGAGCAGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAg                    <  1:383960/150‑1 (MQ=17)
                                           tCGCTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTaaggg                   >  4:1528599/1‑147 (MQ=2)
                                           tCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGt                   <  1:210999/150‑1 (MQ=32)
                                           tCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGt                   >  3:484817/1‑150 (MQ=32)
                                            cGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTg                  <  4:1441785/150‑1 (MQ=32)
                                            cGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTg                  <  1:1191456/150‑1 (MQ=32)
                                              cTCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGt                >  3:911301/1‑150 (MQ=2)
                                              cTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGt                >  4:567717/1‑150 (MQ=32)
                                               tCAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTa               >  2:154088/1‑150 (MQ=2)
                                                cAACGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTAc              <  2:226657/150‑1 (MQ=2)
                                                cAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTg                                                          <  1:734557/106‑1 (MQ=33)
                                                cAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTg                                                          >  2:734557/1‑106 (MQ=33)
                                                cAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAc              <  2:1756823/150‑1 (MQ=32)
                                                aaaCGTATAAAAGGTACTCCGGGAATAACAGGGTGATACCGCCCAAGAGTTCCTAACGACCGCAGTGCTTGTCACCTCGATGTCGGCTCATCACATCCT‑CAACTGAAGTAGGCCCCACGGGAATGGCTGTGCGCCATTTAAGGtcagacg             >  2:848278/2‑144 (MQ=2)
                                                 aaCGGATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTAcg             >  1:740142/1‑150 (MQ=2)
                                                 aaCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCaa                                             <  2:158647/118‑1 (MQ=32)
                                                 aaCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcg             >  3:1455953/1‑150 (MQ=32)
                                                  aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgc            >  2:11968/1‑150 (MQ=32)
                                                  aCGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgc            <  4:1219692/150‑1 (MQ=32)
                                                   cGGATAAAGGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTAcgcg           <  1:1036895/150‑1 (MQ=17)
                                                    ggATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGa          <  3:1449434/150‑1 (MQ=2)
                                                    ggATAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGa          <  3:1449242/150‑1 (MQ=2)
                                                    ggATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGa          <  3:898147/150‑1 (MQ=32)
                                                       tAAAAGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCt       >  1:1101901/1‑150 (MQ=2)
                                                       tAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCt       <  1:11968/150‑1 (MQ=32)
                                                         aaaGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTgg     >  4:327721/1‑150 (MQ=2)
                                                         aaaGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTgg     <  3:1008224/150‑1 (MQ=2)
                                                         aaaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg     <  4:1033575/150‑1 (MQ=32)
                                                         aaaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg     <  2:1426203/150‑1 (MQ=32)
                                                         aaaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTgg     <  1:1167089/150‑1 (MQ=32)
                                                          aaGTTACCCCGGGGATAACAGGCTTATCTCCCCCAAGAGTTCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCCGTAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTggg    >  2:2599302/1‑150 (MQ=2)
                                                          aaGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTggg    >  1:2009723/1‑150 (MQ=32)
                                                           aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt   <  4:1018047/150‑1 (MQ=32)
                                                           aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt   >  4:1629750/1‑150 (MQ=32)
                                                           aGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGt   >  1:1803783/1‑150 (MQ=32)
                                                            ggTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGtt  <  1:2600963/150‑1 (MQ=32)
                                                             |                                                                                                                                                    
AAAGCAGGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGAGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTT  >  NC_000913_3_hsa_tpiA/3946079‑3946288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑