| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913_3_hsa_tpiA | 2,106,741 | A→T | 100% | S162S (TCT→TCA) | wbbH ← | O‑antigen polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_hsa_tpiA | 2,106,741 | 0 | A | T | 100.0% | 133.0 / ‑6.7 | 44 | S162S (TCT→TCA) | wbbH | O‑antigen polymerase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base T (22/21); total (23/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAA > NC_000913_3_hsa_tpiA/2106597‑2106881 | tctcTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGGAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATTGTTTTAGag > 2:311527/1‑148 (MQ=255) aTTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCaaa < 2:391792/149‑1 (MQ=255) ttAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCACTATTCAGTATAATTGCAAATATGAATACAATAAAAACAAGTAAATTAAATGTTTTACTTACTTTTGTATTAGTAAATT‑TTTTTGACCaaat > 2:455148/1‑149 (MQ=255) aatctaTGAAAACATAAGAGAAGACAACCAAAAAGAATACTTTTTTACCACTATTCAATATAATTAAAAAGATGAATACAATAAAATCAAGTAAAGTAAAGGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTTACCAAAtaaataaa < 2:197727/144‑1 (MQ=255) gAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAag < 1:527552/149‑1 (MQ=255) aTGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAagta < 2:491087/149‑1 (MQ=255) tAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAg > 2:593619/1‑149 (MQ=255) atgatAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGAt < 1:668184/149‑1 (MQ=255) atAACCATAAGTACAATTTGCTTACCATTATTCAGTATAAGTGCAAAGATGAATACAATAAAAACAAGTAAAGGAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTgg < 2:581768/149‑1 (MQ=255) atAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAATGATGATTACAATAAAAACAATCAAAGTAAATGTTTTACTTATCTTTGTATTAGTAAATT‑TTTTTGACCAAATCAATATAGCAAAAGTAGTAAGACTGATTgg > 2:198096/1‑149 (MQ=255) ccATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAGAAACAAGTAAAGTAAATGTTTTACTTTCCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAATCAGAATTAGTTAGAATGTTTGTCTga > 2:618951/1‑148 (MQ=255) cATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCa > 1:339052/1‑149 (MQ=255) tAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAATTAAATCAACCAAAACTAGTTAGAATGATTGCCTGCATg > 2:366885/1‑149 (MQ=255) aGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTAt > 1:457758/1‑149 (MQ=255) gACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATg > 2:559786/1‑149 (MQ=255) aCAAATTTCTTAACAGTATTAAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTATACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGc < 2:419947/149‑1 (MQ=255) atctGCCTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTga < 1:216930/146‑1 (MQ=255) tttGCTTACCATGATTCTATATAATTGCAAAGATTAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTgag < 1:40344/149‑1 (MQ=255) ccAGTATTCAGTATAATTGCAAAGATGACTACTTTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCt > 2:147375/1‑149 (MQ=255) cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt > 1:554767/1‑149 (MQ=255) cAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCtt < 1:289053/149‑1 (MQ=255) tatgtcatataATAGAAAAGATGAATACAATAAAAACAAGTAAAGTAAATGATTTACTTACCTTTGTATTAGTAAATT‑TTTTTCACCAAATCAATAAAGCAAAAGTATTGAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGAt < 2:664793/142‑1 (MQ=255) aGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTc > 1:106820/1‑149 (MQ=255) aaacacAAAAAAGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCaa < 1:237058/144‑1 (MQ=255) tGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACAt < 2:373883/149‑1 (MQ=255) tGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGGGTC‑TTCAACAt > 1:139975/1‑149 (MQ=255) aGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTCAAATAAGTAAATT‑TTATTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCa > 1:237073/1‑149 (MQ=255) aTAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCATCATCTCTTATCaa > 2:649347/1‑149 (MQ=255) tAAAAACAAGTAAAGTAAAGGTTTTACTTAGCTTTATATTAGTAACTT‑TGTTTGACCGAATAAAGAAAGCAAAAGTAGTTAGTATGATTGGCTGCATGTATGGTGAGAGATTTCTTGAGGTGTC‑TTCAACATCAGCATCTCTTATCaaa < 2:304196/149‑1 (MQ=255) aaaacCAATCAAAGTAAATGTTCAACTCACGATTGTATTAGTAAATT‑TTTTTTACCAAATAAATAAATCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTT‑ATAACACTTTTTACATCAGCATCTCTTATCAAAt < 2:667958/149‑1 (MQ=255) aaaaaCAGCGAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTACAATGATTGGCTCCACGTATGCTGAGAAATCTCCTGATCTGCC‑TCCAACATCACCATCTCTTATCAAAt < 2:330460/149‑1 (MQ=255) aaaaCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGAAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAAtt > 1:281985/1‑149 (MQ=255) aaaaCAAGTAAAGTAAATGTTTTACATACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAAtt < 1:426494/149‑1 (MQ=255) aCAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCat < 2:690716/149‑1 (MQ=255) taaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGc < 1:102137/149‑1 (MQ=255) aaagtaaaTGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAATCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCt < 1:655020/149‑1 (MQ=255) agtaaaTGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTTAGAAATTTTTAGTTGTAAC‑GACAAGATCAGCATCTCTTATCAAATTCATATAGCtaa > 1:645367/1‑149 (MQ=255) aaTGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCtaagtaa > 1:258791/1‑149 (MQ=255) ttacCTTTGTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTACCg > 1:17427/1‑149 (MQ=255) tttGTATTAGTAAATT‑TTTTTTTTTTATTTTTTAAAGCAAAAATAGTTAGAATGATTGGCTGCATGTATGCTGGGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCTTTTATCTATTTAATCTAGTCCAGAACtt > 2:266597/1‑148 (MQ=255) gTATTAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTgcga > 2:108129/1‑146 (MQ=255) tATTAGTAAATT‑TTTTTGAACAATTAAGTAAAGCAAAAGGAGATAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATATCTAATTAACCTAGTCCCGAACTgttcg > 2:81812/1‑145 (MQ=255) ttAGTAAATT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTAGATGTGTC‑TTCATCATCAGCATCTCTTATCAAATTCATATACCTAAGAAAGCTAGTCCCGAAACGGTAGtt > 2:95909/1‑149 (MQ=255) aaTT‑TTTTTGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTAAGCTTAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTaa < 2:470739/149‑1 (MQ=255) | TCTATTAACACCTACGATGAATGCATAAGAGATGATAACCATAAAGACAATTTGCTTACCAGTATTCAGTATAATTGCAAAGATGAATACAATAAAAACAAGTAAAGTAAATGTTTTACTTACCTTTGTATTAGTAAATT‑TTTTAGACCAAATAAATAAAGCAAAAGTAGTTAGAATGATTGGCTGCATGTATGCTGAGAAATTTCTTGATGTGTC‑TTCAACATCAGCATCTCTTATCAAATTCATATAGCTAAGTAAGCTAGTCCCGAACTGGTAGTTACTTAA > NC_000913_3_hsa_tpiA/2106597‑2106881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |