BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	389,925	(T)_6→7	intergenic (‑198/‑326)	hemB ← / → yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	389,919	1	.	T	99.1%	401.5 / ‑5.7	110	intergenic (‑192/‑332)	hemB/yaiT	5‑aminolevulinate dehydratase (porphobilinogen synthase)/pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
Reads supporting (aligned to +/- strand): ref base . (0/0); major base T (56/53); minor base G (1/0); total (57/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 118 total aligned reads displayed.

GGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATA  >  NC_000913_3_hsa_tpiA/389776‑390061

gggTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttt                                                                                                                                          >  2:660083/1‑150 (MQ=255)

  gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                        >  4:615276/1‑148 (MQ=255)

  gTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttg                                                                                                                                        <  2:971364/150‑3 (MQ=255)

   tAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttgg                                                                                                                                       >  1:996999/1‑147 (MQ=255)

     gggATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAACAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑tttttttggag                                                                                                                                     <  1:705413/150‑6 (MQ=255)

       gATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGtt                                                                                                                                    >  2:639079/1‑150 (MQ=255)

       gATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGtt                                                                                                                                    <  3:202025/150‑1 (MQ=255)

        aTGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGttt                                                                                                                                   >  3:172152/1‑150 (MQ=255)

         tGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTg                                                                                                                                  <  4:372263/150‑1 (MQ=255)

           ttttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGt                                                                                                                                >  2:885315/1‑150 (MQ=255)

           ttttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGt                                                                                                                                >  3:541156/1‑150 (MQ=255)

            tttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGtt                                                                                                                               >  4:652923/1‑150 (MQ=255)

             ttACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTg                                                                                                                              <  3:652923/150‑1 (MQ=255)

                cGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTgcgc                                                                                                                           <  4:318382/150‑1 (MQ=255)

                 gAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgct                                                                                                                          <  3:369741/150‑1 (MQ=255)

                 gAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTAGAGTTTGTTTGCgct                                                                                                                          >  2:427634/1‑150 (MQ=255)

                  aaaGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctg                                                                                                                         >  2:471378/1‑150 (MQ=255)

                    aGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctgct                                                                                                                       <  2:503431/150‑1 (MQ=255)

                    aGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctgct                                                                                                                       <  3:486189/150‑1 (MQ=255)

                        gTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTTATTTTTTTTTATAGCTTTTTGTTATTATTTTTTTGTATGTTGGTTGCGTTGTTATaa                                                                                                                   >  4:216805/1‑150 (MQ=255)

                           gcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAAtt                                                                                                                <  2:356578/150‑1 (MQ=255)

                           gcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAAtt                                                                                                                <  4:672973/150‑1 (MQ=255)

                            cgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                               >  4:489218/1‑150 (MQ=255)

                            cgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTg                                                                                                               <  2:1061324/150‑1 (MQ=255)

                              aTGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaa                                                                                                             <  1:557113/150‑1 (MQ=255)

                              aTGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTGTGGTTGCGCTGCTATAAATTGaa                                                                                                             <  2:524059/150‑1 (MQ=255)

                                    aCAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGAAGTTTGGTTGCGCTGCTATAAATTGAATAATTa                                                                                                       <  2:820078/150‑1 (MQ=255)

                                     aaagaagAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAACAATTaa                                                                                                      <  4:248049/149‑1 (MQ=255)

                                      aagaagAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTAGTTGCGCTGCTATAAATTGAATAATTaaa                                                                                                     >  4:659650/1‑150 (MQ=255)

                                           gagaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                                <  2:195955/150‑1 (MQ=255)

                                           gagaATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACttt                                                                                                >  1:504759/1‑150 (MQ=255)

                                              aaTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATGTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtt                                                                                             <  2:858982/150‑1 (MQ=255)

                                                 ggAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCa                                                                                          <  1:639079/150‑1 (MQ=255)

                                                  gAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCat                                                                                         <  1:525472/150‑1 (MQ=255)

                                                     gagagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTGATCTTTATTTATAGCTGTTGGTTATTAGTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTttgtttggaaca                                                                                      >  4:539473/1‑143 (MQ=255)

                                                      agagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTc                                                                                     <  1:885315/150‑1 (MQ=255)

                                                       gagGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCa                                                                                    >  2:742200/1‑150 (MQ=255)

                                                         ggCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATa                                                                                  <  1:1113113/150‑1 (MQ=255)

                                                          gCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGTGCTTCTATAAATTGAATAAATAAAACTTTGTTGCATATTCATAg                                                                                 >  2:127100/1‑150 (MQ=255)

                                                          gCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAg                                                                                 >  2:949548/1‑150 (MQ=255)

                                                            cAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGa                                                                               >  2:804515/1‑150 (MQ=255)

                                                               gACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTATGAGGatat                                                                            >  1:582119/1‑150 (MQ=255)

                                                                 cATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATAttt                                                                          <  2:761153/150‑1 (MQ=255)

                                                                     tttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCt                                                                      >  2:240882/1‑150 (MQ=255)

                                                                      ttttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTg                                                                     >  3:645394/1‑150 (MQ=255)

                                                                        ttAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgt                                                                   <  2:32187/150‑1 (MQ=255)

                                                                          aaTAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgttt                                                                 >  1:496792/1‑150 (MQ=255)

                                                                             aGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtg                                                              <  3:439221/150‑1 (MQ=255)

                                                                             aGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtg                                                              >  2:388355/1‑150 (MQ=255)

                                                                               aaaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtt                                                            <  1:659922/150‑1 (MQ=255)

                                                                               aaaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtt                                                            <  1:660083/150‑1 (MQ=255)

                                                                               aaaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgtt                                                            >  2:113119/1‑150 (MQ=255)

                                                                                aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgttt                                                           <  3:598539/150‑1 (MQ=255)

                                                                                aaCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgtttgtgttt                                                           <  4:315194/150‑1 (MQ=255)

                                                                                 aCAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGTTTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGc                                                                                           >  4:533022/1‑117 (MQ=255)

                                                                                   aGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGtttgtgtttgtg                                                        >  4:359133/1‑150 (MQ=255)

                                                                                    gCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGATTTTGGTTGCGCTGCTATAAATTTAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGTattgtgtttgtgc                                                       >  2:401776/1‑149 (MQ=255)

                                                                                    gCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGa                                                       <  4:36964/150‑1 (MQ=255)

                                                                                      aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaa                                                                                                             <  1:224211/94‑1 (MQ=255)

                                                                                      aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGaa                                                                                                             >  2:224211/1‑94 (MQ=255)

                                                                                      aataatTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGata                                                     >  3:405361/1‑150 (MQ=255)

                                                                                           ttttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGc                                                                                           >  2:758894/1‑107 (MQ=255)

                                                                                           ttttATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGc                                                                                           <  1:758894/107‑1 (MQ=255)

                                                                                           ttatataTTCACTGAAAATATTTTTAATCTATATTTATAGCTGTTTGTTATTATTTTTTTGGAGTTTGGTTGCACTGTTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGAtatttat                                                <  2:241356/147‑1 (MQ=255)

                                                                                                tatTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                                           >  1:130772/1‑150 (MQ=255)

                                                                                                tatTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                                           <  1:1132690/150‑1 (MQ=255)

                                                                                                    cACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTAt                                       <  4:645394/150‑1 (MQ=255)

                                                                                                     aCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCCTAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATg                                      >  1:302818/1‑150 (MQ=255)

                                                                                                     aCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTAt                                                                        <  3:186498/116‑1 (MQ=255)

                                                                                                     aCTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTAt                                                                        >  4:186498/1‑116 (MQ=255)

                                                                                                        gAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCtt                                   <  1:996694/150‑1 (MQ=255)

                                                                                                          aaaTATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCa                                 >  4:5270/1‑150 (MQ=255)

                                                                                                            atatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGTGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaa                               <  1:640868/150‑1 (MQ=255)

                                                                                                            atatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaa                               >  1:430497/1‑150 (MQ=255)

                                                                                                             tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgt                                                                   >  2:1009993/1‑113 (MQ=255)

                                                                                                             tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGgt                                                                   <  1:1009993/113‑1 (MQ=255)

                                                                                                             tatTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCaaaa                              <  3:489218/150‑1 (MQ=255)

                                                                                                              atTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                                           >  1:183065/1‑136 (MQ=255)

                                                                                                              atTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                                           <  2:183065/136‑1 (MQ=255)

                                                                                                                ttttAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa                           >  1:398704/1‑150 (MQ=255)

                                                                                                                   tAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaaa                        <  2:1070986/150‑1 (MQ=255)

                                                                                                                   tAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaaa                        <  3:48630/150‑1 (MQ=255)

                                                                                                                   tAATCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaaa                        <  1:742200/150‑1 (MQ=255)

                                                                                                                     aTCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAAtt                      <  3:615276/150‑1 (MQ=255)

                                                                                                                      tCTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTa                     >  1:414032/1‑150 (MQ=255)

                                                                                                                       cTTTATTTATAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTAt                    >  3:202279/1‑150 (MQ=255)

                                                                                                                        tttatttaTAGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTTCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTTTTATTTATTATGCGTATGCTTCATATTCTAAATTAtt                   >  2:995350/1‑149 (MQ=255)

                                                                                                                        tttatttaTAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTAtt                   >  3:196104/1‑149 (MQ=255)

                                                                                                                        tttatttaTAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATc                   >  1:498510/1‑150 (MQ=255)

                                                                                                                             ttatAGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCaa              <  1:388355/150‑1 (MQ=255)

                                                                                                                              tataGCTGTTGGTTATTATTTTTTTTTTTTTTTGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAAc             >  4:495629/1‑150 (MQ=255)

                                                                                                                               ataGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCgctg                                                                                                                         <  3:88261/41‑1 (MQ=255)

                                                                                                                               ataGCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACa            >  4:187409/1‑150 (MQ=255)

                                                                                                                                 aGCTGTTGGTTATTATTTTTTTTTTTTTTTTTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCTTATGCTTCAAAAACAAACTTATCTGCAACATg          >  4:191303/1‑150 (MQ=255)

                                                                                                                                  gCTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGt         >  1:364212/1‑150 (MQ=255)

                                                                                                                                   cTGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGTATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGtt        >  2:709468/1‑150 (MQ=255)

                                                                                                                                    tGTTGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTg       <  4:75100/150‑1 (MQ=255)

                                                                                                                                      ttGGTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGaa     >  1:733163/1‑150 (MQ=255)

                                                                                                                                       tGGTAATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGaaa    <  1:600870/150‑1 (MQ=255)

                                                                                                                                         gTTATTATTTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCGTATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATa  >  1:785588/1‑150 (MQ=255)

GGGTAGGGATGTTTTACGAAAGTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTA‑TTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTGCAACATGTTGAAATA  >  NC_000913_3_hsa_tpiA/389776‑390061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`