Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,743,514	C→T	L20F (CTC→TTC)	mdtK →	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,743,514	0	C	T	100.0%	254.7 / NA	71	L20F (CTC→TTC)	mdtK	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/32);  total (39/32)

AATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGC  >  NC_000913_3_hsa_tpiA/1743367‑1743656                                                                                                                                                    |                                                                                                                                               aaTAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATtttc                                                                                                                                              <  1:1132149/150‑1 (MQ=255)  aTAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTcg                                                                                                                                             <  3:301702/150‑1 (MQ=255)     gACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCgcgc                                                                                                                                          >  3:204119/1‑150 (MQ=255)       cTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCaa                                                                                                                                        <  1:110575/150‑1 (MQ=255)                cccTGCTTCTTCTTTTTTTTTTTTTTTTAGGCGGATTTGTAGTCTCCCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGACGCGCTTCTGTTATTAGCTTTTGCAATCCCGGTGATTTTCGCGCAAATCGCCCaa                                                                                                                               >  2:916812/1‑150 (MQ=255)                 ccggCTTCTGCTTTTGGCTGCCCATTCAGGCGGCGGGTGAGTTTCCGAGATAACTACAACTAAAGGGTGTACGAGTGCAGGACGAGATCAGTGAAGCACGTCTGTTATTTGCATGAGCAGACCCGGCGATTTTGGCGCGAATCGCCCaaa                                                                                                                              >  4:91409/4‑150 (MQ=255)                       tcttcttTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGa                                                                                                                        <  3:91409/150‑1 (MQ=255)                             ttttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGtt                                                                                                                  <  2:635697/150‑1 (MQ=255)                              tttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGttt                                                                                                                 <  1:1140943/150‑1 (MQ=255)                              tttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGttt                                                                                                                 >  3:1372/1‑150 (MQ=255)                               ttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGtttt                                                                                                                >  1:949098/1‑150 (MQ=255)                               ttGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGtttt                                                                                                                >  1:948878/1‑150 (MQ=255)                                       cATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATAc                                                                                                        >  4:94567/1‑150 (MQ=255)                                          tCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                                     <  2:282807/150‑1 (MQ=255)                                            aGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGa                                                                                                                        >  1:774908/1‑129 (MQ=255)                                            aGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGa                                                                                                                        <  2:774908/129‑1 (MQ=255)                                            aGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGa                                                                                                   <  1:935704/150‑1 (MQ=255)                                             ggcggcTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACTGTGAt                                                                                                  <  3:1442/150‑1 (MQ=255)                                             ggcggcTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACTGTGAt                                                                                                  >  4:1442/1‑150 (MQ=255)                                                 gcTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcg                                                                                              >  3:623109/1‑150 (MQ=255)                                                     tttAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATggcgggcg                                                                                          >  2:871231/1‑150 (MQ=255)                                                            tctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGtttt                                                                                                                >  1:1031844/1‑121 (MQ=255)                                                            tctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGtttt                                                                                                                <  2:1031844/121‑1 (MQ=255)                                                            tctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                                    >  3:675031/1‑149 (MQ=255)                                                            tctcATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCtata                                                                                    <  4:675031/149‑1 (MQ=255)                                                                atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                                     <  2:1079089/128‑1 (MQ=255)                                                                atatAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGt                                                                                                     >  1:1079089/1‑128 (MQ=255)                                                                atatAACGACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGcc                                                                               <  4:111172/150‑1 (MQ=255)                                                                  ataACTACAAATAAAAGGTGTTCACGTGCAGCAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCAc                                                                             >  2:1150358/1‑150 (MQ=255)                                                                     aCTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCGGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGa                                                                          <  3:269625/150‑1 (MQ=255)                                                                          aaaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                                     <  1:1058618/150‑1 (MQ=255)                                                                          aaaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                                     >  1:710747/1‑150 (MQ=255)                                                                          aaaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATgg                                                                     >  1:357531/1‑150 (MQ=255)                                                                           aaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGc                                                                                >  2:21046/1‑138 (MQ=255)                                                                           aaTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGc                                                                                <  1:21046/138‑1 (MQ=255)                                                                            aTAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATGAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATggcg                                                                   >  3:592270/1‑150 (MQ=255)                                                                               aaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                                                 >  2:372925/1‑117 (MQ=255)                                                                               aaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATg                                                                                                 <  1:372925/117‑1 (MQ=255)                                                                               aaaGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGgcggcg                                                                <  2:710747/150‑1 (MQ=255)                                                                                 aGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGt                                                              >  2:387062/1‑150 (MQ=255)                                                                                   gtgtTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                                            <  4:623109/150‑1 (MQ=255)                                                                                   gtgtTCACGTGCAGAAGTATATCAGTGAAGCGCGTCCGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCg                                                            >  1:314751/1‑150 (MQ=255)                                                                                         aCGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCTGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                                      >  2:459143/1‑150 (MQ=255)                                                                                         aCGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                                      <  4:204119/150‑1 (MQ=255)                                                                                         aCGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                                      >  3:621807/1‑150 (MQ=255)                                                                                             gCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAc                                                  >  1:450991/1‑150 (MQ=255)                                                                                                    tataTCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATc                                           <  4:274721/150‑1 (MQ=255)                                                                                                      tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                                         <  2:364576/150‑1 (MQ=255)                                                                                                      tatCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTg                                         >  2:591072/1‑150 (MQ=255)                                                                                                          aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                                     >  1:860244/1‑150 (MQ=255)                                                                                                          aGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCtt                                     >  1:1134962/1‑150 (MQ=255)                                                                                                           gTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTc                                    >  1:815649/1‑150 (MQ=255)                                                                                                             gAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCg                                  <  4:450035/150‑1 (MQ=255)                                                                                                                gcgcGTCTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCg                               >  4:432270/1‑150 (MQ=255)                                                                                                                   cgTCTGTTATTAGCCTTAGCAACCCCGGGGATTTTCGCGCATATCGCACAAACTGGGATGGGTTTTGTCGATACCGTGAGGGCGGGCGGCTATAGGGCCTCCGACATGGCGGCAGTGGCGATCTGGACGTCTGTCTGGCGTCCGGCGagc                            <  2:8046/150‑3 (MQ=255)                                                                                                                      cTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc                         >  3:212562/1‑150 (MQ=255)                                                                                                                      cTGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGATTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctc                         >  4:17310/1‑150 (MQ=255)                                                                                                                       tGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTa                                                         >  3:485625/1‑117 (MQ=255)                                                                                                                       tGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTa                                                         <  4:485625/117‑1 (MQ=255)                                                                                                                       tGTTATTAGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCctct                        >  1:924296/1‑150 (MQ=255)                                                                                                                         ttattaGCATTAGCAATCCCGGTTATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt                      <  4:657737/150‑1 (MQ=255)                                                                                                                         ttattaGCATTAGCAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCttt                      >  3:524479/1‑150 (MQ=255)                                                                                                                          tcttagcattagcaATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGCTGGCGGGTGGCTCTAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTg                     <  1:1031607/148‑1 (MQ=255)                                                                                                                          tattaGCATTAGCAATCCCGGTGACTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTAATGCTATCGGTCTTCCGGCGATCCTCGTTg                     <  2:836033/150‑1 (MQ=255)                                                                                                                           attagcattagcAATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTgg                    <  1:803112/150‑1 (MQ=255)                                                                                                                              agcattagcaATCCCGGTGATTTTTGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATTCTCTTTGGTcc                 >  4:637268/1‑149 (MQ=255)                                                                                                                               gcattagcaATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCAc                >  3:287532/1‑150 (MQ=255)                                                                                                                                     gcaATCCCGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGActg          <  3:266125/150‑1 (MQ=255)                                                                                                                                            cGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                                      >  3:624487/1‑99 (MQ=255)                                                                                                                                            cGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCg                                                      <  4:624487/99‑1 (MQ=255)                                                                                                                                            cGGTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTgg   <  3:94567/150‑1 (MQ=255)                                                                                                                                             ggTGATTTTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGc  >  2:505958/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                               AATAGACTGAATTTCCCCTGCTTCTTCTTTTTGCTGCCCATTCAGGCGGCTTTTTAGTCTCTCATATAACTACAAATAAAAGGTGTTCACGTGCAGAAGTATATCAGTGAAGCGCGTCTGTTATTAGCATTAGCAATCCCGGTGATTCTCGCGCAAATCGCCCAAACTGCGATGGGTTTTGTCGATACCGTGATGGCGGGCGGCTATAGTGCCACCGACATGGCGGCGGTCGCTATCGGTACTTCTATCTGGCTTCCGGCGATCCTCTTTGGTCACGGACTGCTGCTGGC  >  NC_000913_3_hsa_tpiA/1743367‑1743656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑