Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,756,430 G→T G245C (GGC→TGC)  pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,756,4300GT98.6% 251.3 / NA 72G245C (GGC→TGC) pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (32/39);  minor base C (0/1);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGC  >  NC_000913_3_hsa_tpiA/1756283‑1756575
                                                                                                                                                   |                                                                                                                                                 
aaGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGc                                                                                                                                                 <  1:711388/150‑1 (MQ=255)
aaGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGc                                                                                                                                                 <  4:40442/150‑1 (MQ=255)
    gTTCTGACGTTATCGAAATCAGTGCGCCCCTGAAAGCGCACGCCGGAGAACACATCCTCTCCTTATACCAATTCGTACTCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGAcc                                                                                                                                             <  3:427708/150‑1 (MQ=255)
       cTGACGTTATCGAAAGCCGGGGGCACCTGAAAGCGCACGGCGGCGAAAGCATCCACATCATCTCCAAAATCGAAAACCATGAAGGCCTCAACAACTTCGACGAAAGCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTgg                                                                                                                                          <  2:969791/150‑1 (MQ=255)
            gTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTa                                                                                                                                     <  2:354649/150‑1 (MQ=255)
                  gAAATCCGTGAGCACCTGAAAGCGAACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATCGTTGCGCATTGCTACCTGGGTTTAGAAATc                                                                                                                               >  2:959826/1‑150 (MQ=255)
                   aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATcc                                                                                                                              >  3:386435/1‑150 (MQ=255)
                    aaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTgg                                                                                                                                          >  4:180979/1‑137 (MQ=255)
                    aaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTgg                                                                                                                                          <  3:180979/137‑1 (MQ=255)
                     aTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCg                                                                                                                            <  4:234636/150‑1 (MQ=255)
                        cGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTa                                                                                                                         <  1:374845/150‑1 (MQ=255)
                                 cagAAAGCACACGGGGGCGAAAACATCCACATCATCTCCGAATTCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAAGCCTCGAAACCGCTGACGGTATCATGATTGCGAGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGtt                                                                                                                <  4:662713/148‑1 (MQ=255)
                                   gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTAt                                                                                                              <  1:394885/150‑1 (MQ=255)
                                   gAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTAt                                                                                                              >  1:860320/1‑150 (MQ=255)
                                    aaaGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATc                                                                                                             <  1:342687/150‑1 (MQ=255)
                                               cggcgAAAACATCTACATCATATCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCagaa                                                                                                  <  4:518388/150‑1 (MQ=255)
                                               cggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCagaa                                                                                                  <  1:238865/150‑1 (MQ=255)
                                                ggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCagaag                                                                                                 <  3:586426/150‑1 (MQ=255)
                                                   gAAAACATCCACATCATCTCCAATATCGAATACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatg                                                                                              <  3:219918/150‑1 (MQ=255)
                                                    aaaaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTa                                                                                                                                     <  1:252423/110‑1 (MQ=255)
                                                    aaaaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTa                                                                                                                                     >  2:252423/1‑110 (MQ=255)
                                                      aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgatg                                                                                           <  2:860320/150‑1 (MQ=255)
                                                            cacaTCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaa                                                                                     <  4:386435/150‑1 (MQ=255)
                                                             acaTCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATTATCGaaa                                                                                    >  3:182579/1‑150 (MQ=255)
                                                             acaTCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATTATCGaaa                                                                                    >  3:182560/1‑150 (MQ=255)
                                                             acaTCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaa                                                                                    >  1:84158/1‑150 (MQ=255)
                                                              catcatCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGaaaa                                                                                   >  1:995099/1‑150 (MQ=255)
                                                                 catcTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATg                                                                                >  4:58806/1‑150 (MQ=255)
                                                                     tcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGTAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATc                                                                            >  4:664947/1‑150 (MQ=255)
                                                                     tcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATc                                                                            >  1:215683/1‑150 (MQ=255)
                                                                      ccAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGCGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGACGATGCTGATCGAAAAAGGTATcc                                                                           >  2:819495/1‑150 (MQ=255)
                                                                       cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAgaagaa                                                                                                                   <  2:7819/109‑1 (MQ=255)
                                                                       cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAgaagaa                                                                                                                   >  1:7819/1‑109 (MQ=255)
                                                                       cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg                                                                          >  2:318722/1‑150 (MQ=255)
                                                                       cAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg                                                                          >  4:134170/1‑150 (MQ=255)
                                                                               aaaaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTa                                                                  >  2:987416/1‑150 (MQ=255)
                                                                                aaaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaa                                                                 >  2:482037/1‑150 (MQ=255)
                                                                                 aaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGGAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa                                                                <  3:558064/150‑1 (MQ=255)
                                                                                 aaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATcc                                                                           <  1:1009976/139‑1 (MQ=255)
                                                                                 aaCCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATcc                                                                           >  2:1009976/1‑139 (MQ=255)
                                                                                     aGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCg                                                            <  3:664947/150‑1 (MQ=255)
                                                                                         aGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTAt                                                        <  3:58806/150‑1 (MQ=255)
                                                                                           gCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCa                                                      <  2:215683/150‑1 (MQ=255)
                                                                                            ccTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCAc                                                     >  2:315948/1‑150 (MQ=255)
                                                                                              tCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                   >  2:784801/1‑150 (MQ=255)
                                                                                               caacaaCTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGc                                                  >  1:511213/1‑150 (MQ=255)
                                                                                               caacaaCTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGc                                                  <  1:784801/150‑1 (MQ=255)
                                                                                                 acaacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGa                                                <  2:1040350/150‑1 (MQ=255)
                                                                                                  caacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                                          >  1:1037098/1‑91 (MQ=255)
                                                                                                  caacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTc                                                                                                          <  2:1037098/91‑1 (MQ=255)
                                                                                                  caacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAc                                               >  2:667573/1‑150 (MQ=255)
                                                                                                   aacTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGAcc                                              >  4:446194/1‑150 (MQ=255)
                                                                                                      ttCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                                                                                                       >  1:592309/1‑90 (MQ=255)
                                                                                                      ttCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                                                                                                       <  2:592309/90‑1 (MQ=255)
                                                                                                         gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga                                                                                             >  1:100349/1‑97 (MQ=255)
                                                                                                         gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga                                                                                             <  2:100349/97‑1 (MQ=255)
                                                                                                           cgaAAGCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGGAGAAATCCCGTTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAATGTCGTTATCACTGCGACCCAGATGCt                                      <  4:115494/150‑1 (MQ=255)
                                                                                                            gaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTg                                     <  2:995099/150‑1 (MQ=255)
                                                                                                            gaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCACGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTg                                     <  2:681042/150‑1 (MQ=255)
                                                                                                                  cacGAAGCCTCTGACAACATCATGGTAGCACGTCGCGACCTGGGTGTAGAAATACCGGTAGAAGAAGTTATCTACGCCAAGCAGATGAAGATCCAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTcc                               <  2:882380/148‑1 (MQ=255)
                                                                                                                      aaGCCTCTAACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGa                           <  4:573000/150‑1 (MQ=255)
                                                                                                                        gCCTCTGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATc                         <  1:987416/150‑1 (MQ=255)
                                                                                                                          ctctGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCaa                       <  1:461741/150‑1 (MQ=255)
                                                                                                                           tctGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTagaaga                                                                                                                    >  1:124598/1‑56 (MQ=255)
                                                                                                                           tctGACGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTagaaga                                                                                                                    <  2:124598/56‑1 (MQ=255)
                                                                                                                               aCGGCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                  >  1:265593/1‑150 (MQ=255)
                                                                                                                                  gCATCATGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCAc               >  4:61077/1‑150 (MQ=255)
                                                                                                                                    atcatGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGc             >  2:765538/1‑150 (MQ=255)
                                                                                                                                     tcatGGTTGCGCGTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGcc            >  2:6789/1‑150 (MQ=255)
                                                                                                                                     tcatGGGTGCGCGTTGCGACCTGGGTGGAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTAAATGAAAAAAAACCCACGcc            <  2:90870/150‑1 (MQ=255)
                                                                                                                                              cgcgTTGCGACCTGGGTGTAGAAATCCCGGTGGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTcgcg   <  2:656449/150‑1 (MQ=255)
                                                                                                                                               gcgTTGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCgcgc  <  1:918140/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGC  >  NC_000913_3_hsa_tpiA/1756283‑1756575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑