Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,755,785	G→T	V30F (GTT→TTT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,755,785	0	G	T	100.0%	92.0 / NA	30	V30F (GTT→TTT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/11);  total (19/11)

TGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAC  >  NC_000913_3_hsa_tpiA/1755643‑1755926                                                                                                                                               |                                                                                                                                              tGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGc                                                                                                                                         >  1:142300/1‑149 (MQ=255)       cAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGa                                                                                                                                   >  1:445526/1‑149 (MQ=255)                   ttCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATg                                                                                                                       <  1:39049/149‑1 (MQ=255)                    tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAACTTGTTTGAATTATGGTACCTAAGTCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATgg                                                                                                                      <  2:576955/149‑1 (MQ=255)                     ctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGAAACAGCCGACCTAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGt                                                                                                                     <  1:493659/149‑1 (MQ=255)                        ccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGGGTCTGAACTTATCTCATGGTGAc                                                                                                                  >  2:604490/1‑149 (MQ=255)                           tCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCAGACCGAAAACCGAATCTGAAGAGATGGTAGCTACCCTGATGTGCCATCGCATGAACTTTATTCGTCTGAACTTCTCTCATGGTGACGAt                                                                                                               >  1:259976/1‑149 (MQ=255)                              ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCa                                                                                                            >  2:276787/1‑149 (MQ=255)                                 tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCGCTCATGGTGACTATGCAGaa                                                                                                         >  1:283914/1‑149 (MQ=255)                                   aaCTTAAAGACTCCCACTGTCAGGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAaca                                                                                                       >  2:249966/1‑149 (MQ=255)                                   aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTCGCTAAAATACTGGACGCTGGCATGAACTTTATGCGTCTACACTTCCCTCATGGGGACTATGCAGAaca                                                                                                       >  2:263681/1‑149 (MQ=255)                                        aaaGACCAAATCTTTCATGAAAAGGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCAGAACTTCTCTCATGGTGACTATGCAGAACACGGTc                                                                                                  >  2:82164/1‑149 (MQ=255)                                          agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAATCGAATCTGATGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAg                                                                                                <  2:512338/149‑1 (MQ=255)                                          agactaagactGTCAGGAAAAAGGCCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACCCGGTCAg                                                                                                >  2:330953/1‑149 (MQ=255)                                           gacgaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                                                                               <  2:88418/149‑1 (MQ=255)                                                    tGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGa                                                                                      >  1:267368/1‑149 (MQ=255)                                                        aTGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCt                                                                                  >  1:639712/1‑149 (MQ=255)                                                          gAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                                                                                <  1:350945/149‑1 (MQ=255)                                                          gAAAAAGACCAAAATTGTTTGCACCAGCGGACCGAAAACCTAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                                                                                <  1:625688/149‑1 (MQ=255)                                                            aaaaGACCAAAATTGTTTGCACCATCGGTCCGAAAAACGAATCTGAAGAGTTATTAGCTAATTTGCTAGGCTCTGACTGTTACTTTATGCGACTGACCTTCTCTCTTGGTGTCTATCCCGAACACGGTCCGCGCATTCACAATCTgcgc                                                                              >  2:278535/1‑149 (MQ=255)                                                                      aaTTGCTTGCACCTTCGGACCGACGACCGAATCTTTAGTGATGTTAGCGAAAATGCTGGCCGCAGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatga                                                                    <  1:426968/149‑1 (MQ=255)                                                                                        aCCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGGGTGTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGGTGGGCAAAACTGGTAAAAccg                                                  >  1:191600/1‑149 (MQ=255)                                                                                           gAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACTTGCTGAGCAAAACTGGTAAACccgccc                                               >  2:266876/1‑148 (MQ=255)                                                                                              aaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTa                                            >  1:381868/1‑149 (MQ=255)                                                                                                      cTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAACCCGCCGCTATCCTGCtt                                    <  2:324973/149‑1 (MQ=255)                                                                                                                      tAAAATGTTGGACGCTGGCATGAACTTCATGCGTCTGAACTTCTCTCATGGTGAGTATGCAGATCACGTTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCgg                    <  2:84149/149‑1 (MQ=255)                                                                                                                       aaaaTGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc                                                   >  1:67420/1‑117 (MQ=255)                                                                                                                       aaaaTGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAcc                                                   <  2:67420/117‑1 (MQ=255)                                                                                                                             cTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg             >  2:486196/1‑149 (MQ=255)                                                                                                                                        cATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAc  >  2:110336/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                              TGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAC  >  NC_000913_3_hsa_tpiA/1755643‑1755926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑