Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	264,098	0	G	T	93.1%	75.2 / NA	29	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (13/14);  minor base C (2/0);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCT  >  NC_000913_3_hsa_tpiA/263959‑264226                                                                                                                                            |                                                                                                                                 ccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCg                                                                                                                         <  1:202966/149‑1 (MQ=255)              aacctcCCCGTGCAGGCCGCTTGTGGGGTGCCTTATTTCAGTTCTCCACTATGGTCAGTTCAGCGTGATGCCCGGCCGGCAGGTATATGGCTCATGGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATATCCGGaa                                                                                                            <  2:306307/146‑1 (MQ=255)              aaTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                            <  1:446917/149‑1 (MQ=255)                tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCGGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACg                                                                                                          >  1:356313/1‑149 (MQ=255)                tctcCCGCTCCAGGCCCCTGGGAGGGTTGCACATTACAGTTCTCCACTATTGTGAGTGCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTACCGGCGGCGGTGGCCGCATACCCCCAGGACGCCGTGGATAGCCGGCAcc                                                                                                          >  2:106340/1‑148 (MQ=255)                  tcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGGTATCCGGGACGTc                                                                                                        >  1:440117/1‑149 (MQ=255)                               cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                           >  1:40836/1‑149 (MQ=255)                                 cTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCCAGGACTGCGGCGATATCCGGATCGTCCCTGCCGCTGTAGTg                                                                                         >  2:365010/1‑149 (MQ=255)                                   ggTAGGGTTGCTCATTACAGTTCTCCACTATTTTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAc                                                                                       <  2:248586/149‑1 (MQ=255)                                                      gTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                    <  1:275024/149‑1 (MQ=255)                                                         ctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTa                                                                 <  1:108529/149‑1 (MQ=255)                                                          tcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAg                                                                                                                                <  2:502434/85‑1 (MQ=255)                                                             aCTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTCGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTg                                                             <  1:631560/149‑1 (MQ=255)                                                              cTATTGTCAGTTCAGGGGGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCCCTGGTATTTGc                                                            <  1:414126/149‑1 (MQ=255)                                                               tATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGGATTTGcc                                                           >  1:215644/1‑149 (MQ=255)                                                                   gTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCGACTGGGCTTTGCCgggg                                                       >  1:641533/1‑147 (MQ=255)                                                                    tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                      >  1:248093/1‑149 (MQ=255)                                                                       gTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGGGTAATGACAGACGCTCCCCTCCCACCGTTTTTTGCCGGTGCatt                                                   >  1:624342/1‑147 (MQ=255)                                                                                   gcgtagcaggcaggTATAGGGCTCGTTGCGGTCCTGGCGGCGATCGGCGTATACCGCTAGGTCTCCGGCGATAGCCGGAACGTCCCTGCTGGTGTAATGACAGACGTTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                       <  2:19366/143‑1 (MQ=18)                                                                                   gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                       <  1:183777/149‑1 (MQ=255)                                                                                   gCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTc                                       <  1:183092/149‑1 (MQ=255)                                                                                    cTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGGTCGGCGTATACCGCTAGGGCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCTACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCg                                      <  2:531567/149‑1 (MQ=255)                                                                                     tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTGCCGTGCCAGTGGTGTTTGCCGGTGCAGTAGCGAAAGATTCgg                                     >  1:461080/1‑149 (MQ=255)                                                                                       atcaGGGAGGTATAGGGGCGATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAACGATTCGGGa                                   >  2:399451/1‑149 (MQ=18)                                                                                            gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                              >  1:542911/1‑149 (MQ=255)                                                                                                         ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAACGATTCGGGGCTCAGGATGCTGGCGGta                 >  1:624792/1‑149 (MQ=255)                                                                                                          cATTGGGGTCCCGGCGGGGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtat                <  2:624645/149‑1 (MQ=255)                                                                                                            ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTACGGCGATATCCGGAACGTCCCTGGCGGTGTAATGACAGAAGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAGGATTCGGGACTGCGGTTCGTGGGGGTatat              >  2:441078/1‑149 (MQ=255)                                                                                                                        cggcgTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCt  >  1:173649/1‑149 (MQ=255)                                                                                                                                            |                                                                                                                                 CCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCT  >  NC_000913_3_hsa_tpiA/263959‑264226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑