Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,726,201	T→G	L60R (CTT→CGT)	nemR →	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,726,201	0	T	G	100.0%	136.5 / NA	41	L60R (CTT→CGT)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/26);  total (15/26)

GAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTG  >  NC_000913_3_hsa_tpiA/1726074‑1726335                                                                                                                                |                                                                                                                                       gAGCAACTTTGCCTGCAACGTGGATTCAACGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGGTTGGCGTTGCCAGGCGTGAGCGTCATTACGCCGCata                                                                                                                   <  2:57306/149‑1 (MQ=255) gAGCAACCTTGCCTGCAACGTCGATTCACCGGGAGGGCGCTAAGCGAATTACTAAAACCCGCTGCAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGACCGTCATTACGCCGCata                                                                                                                   <  2:58502/149‑1 (MQ=255)        tttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAg                                                                                                            <  1:646449/149‑1 (MQ=255)                cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTTCTTCTATCACTACTTTGTCTCTAAAGAAGCGTTTGGCTTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCgactgac                                                                                                    <  1:605648/149‑1 (MQ=255)                 aaCGTGGATTCACCGGGAGGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCgactgact                                                                                                   <  2:102274/149‑1 (MQ=255)                  aCGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGactgactg                                                                                                  >  2:169819/1‑149 (MQ=255)                         ttCACCGGGCTGGGGCTAAGCGAATTACCAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgct                                                                                           <  2:536279/149‑1 (MQ=255)                          tctccGGGGTGTGGATAAGCGAGTTACTAACAACGGCTGAAGTGCGGAACGCGTCCTTCTATCACTACTTTCGCTCTAAGGAAGCGTTTGGCGTTGCCCTGCGGGATCGTCATTACGCGGCATATCACCAGCGACTGACTGAGTtgctg                                                                                          <  2:17555/146‑1 (MQ=255)                          gcagcGGGATGGGGCTAAGCGAATTACTAAAACCCGCTGAAGCTCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgctg                                                                                          <  2:17285/145‑1 (MQ=255)                            aCCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCa                                                                                        >  2:405776/1‑149 (MQ=255)                            aCCGGGATGGGGCGAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCa                                                                                        >  2:417892/1‑149 (MQ=255)                               gggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATc                                                                                     <  2:658196/149‑1 (MQ=255)                                ggATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATcc                                                                                    <  1:169819/149‑1 (MQ=255)                                   tGGGGCTAAGCGAAGTACTAAAAACCCCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGc                                                                                 <  1:243026/149‑1 (MQ=255)                                          aaGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTa                                                                          <  2:332354/149‑1 (MQ=255)                                              ggattgatAAAAACCGCTGAAGGGCCGAAAGGGTCCCTCTATCACTAGTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGCGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAATGTACCTa                                                                      <  2:257936/142‑1 (MQ=255)                                                    cTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGa                                                                <  2:486380/149‑1 (MQ=255)                                                             gCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCACATACt                                                       >  1:123984/1‑149 (MQ=255)                                                              cTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTg                                                      <  1:180238/149‑1 (MQ=255)                                                                 aaGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGACTTACTGCAATCCTGCGAAGGTCACTACCGCGACCGCATACTGGCt                                                   <  1:532233/149‑1 (MQ=255)                                                                 aaGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTATGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGTACGACTAAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATGCTggga                                                   >  1:323931/1‑147 (MQ=255)                                                                   gTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCtta                                                 <  1:417892/149‑1 (MQ=255)                                                                      cagAAAGTGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCttatta                                              <  2:504050/147‑1 (MQ=255)                                                                         aaaGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCACATACTGGCTTATTACCa                                           <  2:123984/149‑1 (MQ=255)                                                                           aGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGc                                         >  1:670254/1‑149 (MQ=255)                                                                           aGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGc                                         <  1:445982/149‑1 (MQ=255)                                                                             ggTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTAcgccgc                                                                                                                      <  1:356393/70‑1 (MQ=255)                                                                             ggTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTAcgccgc                                                                                                                      >  2:356393/1‑70 (MQ=255)                                                                              gTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTCCCGCGACCCCCTACTGGCTTATTACCAACaca                                      >  1:475870/1‑147 (MQ=255)                                                                                 cTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGCCCGCATACTGGCTTATTACCAGCaacccc                                   >  2:653018/1‑145 (MQ=255)                                                                                  ttCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCGTATTACCAGCAAACACt                                  >  1:3556/1‑149 (MQ=255)                                                                                  ttCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGCTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACt                                  >  2:150533/1‑149 (MQ=255)                                                                                    cTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGa                                >  1:364782/1‑149 (MQ=255)                                                                                       tCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAAcc                             >  1:409745/1‑149 (MQ=255)                                                                                            actTTCGCTCTAAAGAAGCGGTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGttt                        <  1:41972/149‑1 (MQ=255)                                                                                                    tctAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgct                                                                                           >  1:521018/1‑74 (MQ=255)                                                                                                    tctAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTtgct                                                                                           <  2:521018/74‑1 (MQ=255)                                                                                                    tctAAAGAAGCGTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGccacca                >  1:145842/1‑149 (MQ=255)                                                                                                     ccaaaGAAGCGTTTGGCGTTGCCAGGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACAt               <  1:303053/147‑1 (MQ=255)                                                                                                               gTTTGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCa     <  1:265758/149‑1 (MQ=255)                                                                                                                  tGGCGTTGCCATGCGTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTg  <  1:306076/149‑1 (MQ=255)                                                                                                                                |                                                                                                                                       GAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGCGTTGCCATGCTTGAGCGTCATTACGCCGCATATCACCAGCGACTGACTGAGTTGCTGCAATCCGGCGAAGGTAACTACCGCGACCGCATACTGGCTTATTACCAGCAAACACTGAACCAGTTTTGCCAACATGGAACCATCAGTG  >  NC_000913_3_hsa_tpiA/1726074‑1726335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑