BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	4,111,506	T→G	intergenic (+821/+91)	cdh → / ← yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	4,111,506	0	T	G	92.1%	675.3 / 30.0	203	intergenic (+821/+91)	cdh/yiiQ	CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand): ref base T (7/9); new base G (96/91); total (103/100)
Fisher's exact test for biased strand distribution p-value = 6.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

GGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAG  >  NC_000913_3_hsa_tpiA/4111359‑4111652

 gAGCACAAACCACCTCGGGGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTgata                                                                                                                                                 <  4:471792/150‑1 (MQ=255)

   gCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAg                                                                                                                                               >  1:312043/1‑150 (MQ=255)

    cacaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGc                                                                                                                                              <  4:353105/150‑1 (MQ=255)

     acaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTACAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACACTGATAAGCg                                                                                                                                             >  3:754722/1‑150 (MQ=255)

       aaaCCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCCACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGaa                                                                                                                                           <  4:1284344/150‑1 (MQ=255)

         accaccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATa                                                                                                                                         >  4:775865/1‑150 (MQ=255)

               tCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGaa                                                                                                                                   <  2:1317693/150‑1 (MQ=255)

                       ggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgccg                                                                                                                           <  4:928595/150‑1 (MQ=255)

                          cggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGccc                                                                                                                        >  1:39894/1‑150 (MQ=255)

                                 ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                                 >  2:646671/1‑150 (MQ=255)

                                  tGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgc                                                                                                                <  2:1635696/150‑1 (MQ=255)

                                       gcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGcagcag                                                                                                           >  1:1414435/1‑150 (MQ=255)

                                         gTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGc                                                                                                                                              <  3:199433/113‑1 (MQ=255)

                                         gTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGt                                                                                                         >  1:1183614/1‑150 (MQ=255)

                                            cAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtg                                                                                                      >  4:1234005/1‑150 (MQ=255)

                                             agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGaa                                                                                                                                           <  4:1403658/112‑1 (MQ=255)

                                             agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGaa                                                                                                                                           >  3:1403658/1‑112 (MQ=255)

                                             agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                     <  1:1139551/150‑1 (MQ=255)

                                             agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                     <  2:414298/150‑1 (MQ=255)

                                                gTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGaa                                                                                                  >  2:1159334/1‑150 (MQ=255)

                                                   ccGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                               <  3:628412/150‑1 (MQ=255)

                                                     gATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGt                                                                                             >  1:1305313/1‑150 (MQ=255)

                                                        gAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGaaa                                                                                                 >  1:2049054/1‑143 (MQ=255)

                                                           cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                       <  2:314202/150‑1 (MQ=255)

                                                              ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                               <  3:90095/139‑1 (MQ=255)

                                                              ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                               >  4:90095/1‑139 (MQ=255)

                                                                ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGaa                                                                                                                                           <  2:2049008/93‑1 (MQ=255)

                                                                ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGa                                                                                                   <  4:1036771/133‑1 (MQ=255)

                                                                   gACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCg                                                                               <  1:890129/150‑1 (MQ=255)

                                                                    aCTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCgg                                                                              <  2:39894/150‑1 (MQ=255)

                                                                        tGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGcaca                                                                          >  2:1194538/1‑150 (MQ=255)

                                                                           ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAg                                                                       <  3:992696/150‑1 (MQ=255)

                                                                           ttCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAg                                                                       >  2:407157/1‑150 (MQ=255)

                                                                               gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                                                   >  3:569785/1‑150 (MQ=255)

                                                                               gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                                                   >  3:385517/1‑150 (MQ=255)

                                                                               gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                                                   >  1:1226603/1‑150 (MQ=255)

                                                                                   gTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAgg                                                                                                                  >  1:1832990/1‑99 (MQ=255)

                                                                                   gTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgct                                                               >  2:1383678/1‑150 (MQ=255)

                                                                                       aTCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                           <  1:1697529/150‑1 (MQ=255)

                                                                                        tCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTg                                                          <  3:775865/150‑1 (MQ=255)

                                                                                         cTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGACGAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGt                                                         >  1:1167398/1‑150 (MQ=255)

                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCCTTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTc                                                        >  1:1974041/1‑150 (MQ=255)

                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTgat                                                                                                                                                  <  2:574800/60‑1 (MQ=255)

                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAAc                                                                                                >  4:204116/1‑110 (MQ=255)

                                                                                          ttCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATag                                                                                         <  2:1915412/117‑1 (MQ=255)

                                                                                           tCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCa                                                       >  2:1893406/1‑150 (MQ=255)

                                                                                             cAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAtt                                                     >  3:324415/1‑150 (MQ=255)

                                                                                             cAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGACACAAAGGCTTTGCTTTTTGTCAtt                                                     <  4:416153/150‑1 (MQ=255)

                                                                                                 ttCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTAtt                                                 <  2:1974041/150‑1 (MQ=255)

                                                                                                   cccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCa                                               <  3:617867/150‑1 (MQ=255)

                                                                                                    ccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                              <  1:2377482/150‑1 (MQ=255)

                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgcttt                                                                  >  2:746400/1‑129 (MQ=255)

                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgcttt                                                                  <  1:746400/129‑1 (MQ=255)

                                                                                                     cccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaaa                                             >  4:1348998/1‑150 (MQ=255)

                                                                                                       cAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGtata                                                                                          <  4:1173134/103‑1 (MQ=255)

                                                                                                       cAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGtata                                                                                          >  3:1173134/1‑103 (MQ=255)

                                                                                                       cAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAAcc                                           >  1:415281/1‑150 (MQ=255)

                                                                                                        aaCGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCt                                          >  2:1755557/1‑150 (MQ=255)

                                                                                                          cGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTc                                        <  4:534644/150‑1 (MQ=255)

                                                                                                           gaagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATaaa                                                                                                                                       <  4:786893/54‑1 (MQ=255)

                                                                                                           gaagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATaaa                                                                                                                                       >  3:786893/1‑54 (MQ=255)

                                                                                                           gaagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                                                <  2:477820/109‑1 (MQ=255)

                                                                                                           gaagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATc                                                                                >  1:477820/1‑109 (MQ=255)

                                                                                                             agaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAg                                     <  2:278173/150‑1 (MQ=255)

                                                                                                              gaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGCTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGc                                    >  1:2099712/1‑150 (MQ=255)

                                                                                                               aaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGTATAAAGGAAGATGGCCGCCGCGCAGGGCAGCAGGTCGGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCg                                   >  2:1559931/1‑150 (MQ=255)

                                                                                                                aCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                                  <  1:407157/150‑1 (MQ=255)

                                                                                                                 cTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAt                                 <  4:621905/150‑1 (MQ=255)

                                                                                                                  ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                     >  1:1454865/1‑81 (MQ=255)

                                                                                                                  ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                     <  2:1454865/81‑1 (MQ=255)

                                                                                                                  ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGaaa                                                                                                 >  2:242360/1‑85 (MQ=255)

                                                                                                                  ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                           >  3:1131468/1‑123 (MQ=255)

                                                                                                                   tCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTc                               >  4:49756/1‑150 (MQ=255)

                                                                                                                     cTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAg                             >  1:1556770/1‑150 (MQ=255)

                                                                                                                         aGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                                                        >  1:2413748/1‑87 (MQ=255)

                                                                                                                             cgcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                              >  4:950764/1‑125 (MQ=255)

                                                                                                                             cgcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGcc                     >  2:904172/1‑150 (MQ=255)

                                                                                                                               ccATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAg                   <  2:786690/150‑1 (MQ=255)

                                                                                                                                cATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACATAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGc                  <  3:1234005/150‑1 (MQ=255)

                                                                                                                                cATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGc                  <  2:1858353/150‑1 (MQ=255)

                                                                                                                                cATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGc                  <  3:420531/150‑1 (MQ=255)

                                                                                                                                 aTTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCt                 <  1:1680666/150‑1 (MQ=255)

                                                                                                                                 aTTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCt                 <  3:1333383/150‑1 (MQ=255)

                                                                                                                                  ttttAATTCTCCACGCTGATAAGCGAGTAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTCTGAAACAGTATAGAGATTCATCGGCACCAAGGCTTTGCTTTTTGTCATTTATTGAAACCTTCAAGCGATTCAGATAGCGCCAGCtt                >  4:1141689/1‑150 (MQ=255)

                                                                                                                                  ttttAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                                  <  3:259265/132‑1 (MQ=255)

                                                                                                                                  ttttAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                                  >  4:259265/1‑132 (MQ=255)

                                                                                                                                   tttAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTa               >  2:2360417/1‑150 (MQ=255)

                                                                                                                                    ttAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGc                                                                                                                     >  1:326661/1‑47 (MQ=255)

                                                                                                                                     tAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAt             >  1:2219079/1‑150 (MQ=255)

                                                                                                                                     tAATTCTCCACGCTGATAAGCGAATAAAGGAAGATCGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAATCCTTCAAGCGATTCAGATAGCGCCAGCTTAAt             <  2:34369/150‑1 (MQ=255)

                                                                                                                                      aaTTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATc            <  2:1726951/150‑1 (MQ=255)

                                                                                                                                        ttCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAt                                                      <  2:2232456/106‑1 (MQ=255)

                                                                                                                                        ttCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCAt                                                      >  1:2232456/1‑106 (MQ=255)

                                                                                                                                           tcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGca                                                                            <  4:392382/81‑1 (MQ=255)

                                                                                                                                           tcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCttttt                                                           >  1:1894307/1‑98 (MQ=255)

                                                                                                                                           tcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc       >  1:600807/1‑150 (MQ=255)

                                                                                                                                            ccACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGc                                                                                                                     >  3:534586/1‑39 (MQ=255)

                                                                                                                                             cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc       >  3:1081012/1‑148 (MQ=255)

                                                                                                                                             cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc       <  4:1081012/148‑1 (MQ=255)

                                                                                                                                             cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCaa     >  1:651751/1‑150 (MQ=255)

GGAGCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAG  >  NC_000913_3_hsa_tpiA/4111359‑4111652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`