Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,544,0211.A79.8% 600.1 / 151.6 198coding (40/1389 nt)narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (27/13);  new base A (81/77);  total (108/90)
Fisher's exact test for biased strand distribution p-value = 7.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_hsa_tpiA1,544,0220CA93.4% 679.1 / 25.5 198L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (8/5);  new base A (100/85);  total (108/90)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_hsa_tpiA/1543879‑1544170
                                                                                                                                                 |||                                                                                                                                                   
ttGAGATCAACCGTAACTACGCTAAATAGCATCCAGACATAGAAGGCAAGAAGTAGACAACTGACTTATATCCAGAGATTTCTTCGAGCAATATGTTCTCCTTTATTTTCCCACAAGGCCGACTTTTCTGGGTC‑CCAGTCGCG‑C‑AAAAGa                                                                                                                                                >  4:691149/1‑150 (MQ=255)
 tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaaga                                                                                                                                               <  1:1651203/150‑4 (MQ=255)
 tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaaga                                                                                                                                               >  3:1003845/1‑147 (MQ=255)
 tGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaaga                                                                                                                                               >  2:788246/1‑147 (MQ=255)
  gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaagat                                                                                                                                              >  2:194279/1‑146 (MQ=255)
    gaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑ACAGTCGCGAA‑AAAAGATaa                                                                                                                                             >  2:2395460/1‑150 (MQ=255)
     aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAAc                                                                                                                                            <  1:148156/150‑1 (MQ=255)
           ggTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGGTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaag                                                                                                                                                >  1:1356101/1‑137 (MQ=255)
           ggTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGGTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑A‑aaaaag                                                                                                                                                <  2:1356101/139‑3 (MQ=255)
           ggTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTAt                                                                                                                                      >  2:51320/1‑150 (MQ=255)
               aCTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTAttttt                                                                                                                                  >  4:1488822/1‑150 (MQ=255)
                   cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCAt                                                                                                                             >  3:710221/1‑150 (MQ=255)
                        aaTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTg                                                                                                                         <  2:702261/150‑1 (MQ=255)
                              aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   >  4:1352666/1‑150 (MQ=255)
                              aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   <  1:456973/150‑1 (MQ=255)
                               tacAGACAAAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTAAAAAAAAAAA‑A‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGcc                                                                                                                  <  4:734492/148‑1 (MQ=255)
                               tCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGcc                                                                                                                  <  1:788246/150‑1 (MQ=255)
                                    acacAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTGCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATAtt                                                                                                             >  4:759647/1‑150 (MQ=255)
                                      acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                           >  1:2041102/1‑150 (MQ=255)
                                        aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         >  3:980728/1‑150 (MQ=255)
                                         gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCa                                                                                                                       >  2:119789/1‑135 (MQ=255)
                                          aaGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                       <  3:681794/150‑1 (MQ=255)
                                           aGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTa                                                                                                                                       >  4:395292/1‑117 (MQ=255)
                                            ggCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                     <  3:874913/150‑1 (MQ=255)
                                              cAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcaca                                                                                                   <  2:2080176/150‑1 (MQ=255)
                                                  aagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGca                                                                                               <  2:1947459/150‑1 (MQ=255)
                                                   agTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGc                                                                                                >  1:37878/1‑148 (MQ=255)
                                                   agTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGc                                                                                                <  2:37878/148‑1 (MQ=255)
                                                    gTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                             >  3:1192403/1‑150 (MQ=255)
                                                       gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAt                                                                                          >  3:908406/1‑150 (MQ=255)
                                                       gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAt                                                                                          <  3:863213/150‑1 (MQ=255)
                                                        aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAtt                                                                                         >  2:362769/1‑150 (MQ=255)
                                                           actgactgATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGt                                                                                      >  2:1774184/1‑150 (MQ=255)
                                                            ctgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac                                                                                              <  2:1842551/141‑1 (MQ=255)
                                                             tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                                    >  4:333777/1‑150 (MQ=255)
                                                                  gaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGa                                                                                 <  1:1859343/148‑1 (MQ=255)
                                                                   atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTAttt                                                                                                                                    <  2:1881928/96‑1 (MQ=255)
                                                                   atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTAttt                                                                                                                                    >  1:1881928/1‑96 (MQ=255)
                                                                     atCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                            >  3:789950/1‑150 (MQ=255)
                                                                      tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                     <  3:495302/124‑1 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTTTTTTTTTTTTTCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCATTGCCATATTGTTCCTCACATGCACACATTGTTAATGATAAAAAGACa                                                                       >  2:589504/1‑150 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATaa                                                                                                                                             <  1:2231679/81‑1 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATaa                                                                                                                                             >  2:2231679/1‑81 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTtct                                                                                                                                >  1:2240483/1‑94 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTtct                                                                                                                                <  2:2240483/94‑1 (MQ=255)
                                                                         agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGAc                                                                        >  3:241047/1‑150 (MQ=255)
                                                                             agcacgccGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaaa                                                                    <  4:566649/143‑1 (MQ=255)
                                                                             aTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTAttttt                                                                                                                                  >  2:1062091/1‑88 (MQ=255)
                                                                             aTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaaaa                                                                    <  1:2161618/150‑1 (MQ=255)
                                                                             aTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTAttttt                                                                                                                                  <  1:1062091/88‑1 (MQ=255)
                                                                               ttcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcac                                                                                              <  1:624851/122‑1 (MQ=255)
                                                                               ttcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           <  1:1166957/141‑1 (MQ=255)
                                                                                tcttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAacac                                                                 <  4:86617/150‑1 (MQ=255)
                                                                                  ttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCAtt                                                                                                                            <  3:393387/89‑1 (MQ=255)
                                                                                       gCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTc                                                                                                      <  2:1144251/106‑1 (MQ=255)
                                                                                          atatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTAttttt                                                                                                                                  <  1:1526635/75‑1 (MQ=255)
                                                                                             tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                                    <  3:323873/118‑1 (MQ=255)
                                                                                             tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCa                                                    <  2:300298/150‑1 (MQ=255)
                                                                                             tGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTGTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCa                                                    <  1:2080323/150‑1 (MQ=255)
                                                                                              gTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           >  1:968065/1‑126 (MQ=255)
                                                                                              gTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           <  2:968065/126‑1 (MQ=255)
                                                                                              gTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                                   <  2:1557716/150‑1 (MQ=255)
                                                                                               ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATaa                                                                                                                                             >  4:1204675/1‑59 (MQ=255)
                                                                                               ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTtct                                                                                                                                <  1:928938/72‑1 (MQ=255)
                                                                                               ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTtct                                                                                                                                >  2:928938/1‑72 (MQ=255)
                                                                                                tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           <  3:1468983/124‑1 (MQ=255)
                                                                                                  tCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                               <  2:2339127/150‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTa                                                                                                                                       <  2:844680/59‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   >  2:626563/1‑79 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacac                                                                                            >  3:273354/1‑102 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacac                                                                                            <  4:273354/102‑1 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAAt                                                                                   >  1:1811883/1‑111 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                            >  3:1103806/1‑150 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGt                                           >  1:114000/1‑150 (MQ=255)
                                                                                                     tttATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                            >  4:976518/1‑150 (MQ=255)
                                                                                                            tCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAg                                     >  4:1232505/1‑150 (MQ=255)
                                                                                                             cccAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                    >  1:749216/1‑150 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGt                                                                                                                     >  1:2103494/1‑67 (MQ=255)
                                                                                                               cAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAAt                                  >  2:491410/1‑150 (MQ=255)
                                                                                                                   aGGCCGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAg                              <  1:885701/150‑1 (MQ=255)
                                                                                                                      ccGGATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGAt                           >  2:1652484/1‑150 (MQ=255)
                                                                                                                       cGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGa                         >  3:1435204/1‑150 (MQ=255)
                                                                                                                        ggATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               <  3:642760/112‑1 (MQ=255)
                                                                                                                         gATTTTCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGaa                        <  3:1279656/150‑1 (MQ=255)
                                                                                                                              tCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg                   <  4:655930/150‑1 (MQ=255)
                                                                                                                              tCTGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg                   >  4:1063831/1‑150 (MQ=255)
                                                                                                                                tGGTTT‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAAt                                                                                   <  3:221641/84‑1 (MQ=255)
                                                                                                                                   ttt‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAg              >  4:1107102/1‑150 (MQ=255)
                                                                                                                                   ttt‑CCAGTCGCGAA‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAg              <  4:881557/150‑1 (MQ=255)
                                                                                                                                        cAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCt          >  2:1372478/1‑150 (MQ=255)
                                                                                                                                        cAGTCGCG‑AAAAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCt          <  2:75567/150‑1 (MQ=255)
                                                                                                                                            tcgcgaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAt       <  1:2047679/143‑1 (MQ=255)
                                                                                                                                              gcgaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTg     >  4:815733/6‑150 (MQ=255)
                                                                                                                                               g‑c‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  2:2111292/150‑1 (MQ=255)
                                                                                                                                                gaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                             <  2:2011079/57‑1 (MQ=255)
                                                                                                                                                gaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcaca                                                                                             >  1:2011079/4‑60 (MQ=255)
                                                                                                                                                gaaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaa   >  2:1451722/4‑150 (MQ=255)
                                                                                                                                                 aaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  1:672351/148‑1 (MQ=255)
                                                                                                                                                 aaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  1:227762/148‑1 (MQ=255)
                                                                                                                                                 aaaaaaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  3:815733/148‑1 (MQ=255)
                                                                                                                                                 |||                                                                                                                                                   
TTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTT‑CCAGTCGCG‑C‑AAAAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_hsa_tpiA/1543879‑1544170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑