Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,159,184 C→A T238N (ACT→AAT)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,159,1840CA98.2% 190.6 / ‑3.3 55T238N (ACT→AAT) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (35/19);  total (35/20)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

AAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTGG  >  NC_000913_3_hsa_tpiA/4159039‑4159327
                                                                                                                                                 |                                                                                                                                               
aaaCTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTa                                                                                                                                             <  1:527096/150‑1 (MQ=255)
     gctgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGcc                                                                                                                                        >  4:318756/1‑150 (MQ=255)
       tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAg                                                                                                                                      >  2:614979/1‑150 (MQ=255)
       tgATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAg                                                                                                                                      >  1:596345/1‑150 (MQ=255)
         aTGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAgcg                                                                                                                                    >  2:398334/1‑150 (MQ=255)
             tGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCACCATGGTGGCCGCAGGTAGCGGGATCAATTTACTGCCAGCgctgg                                                                                                                                >  2:65812/1‑150 (MQ=255)
                     ggTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTgccg                                                                                                                        >  4:488725/1‑150 (MQ=255)
                         aCTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCgg                                                                                                                    >  2:15065/1‑150 (MQ=255)
                             tttGCGCGATCAGGCAATGGGTATCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg                                                                                                                >  2:55194/1‑150 (MQ=255)
                                        aGGCAATGGGTTTCTGTTTTGACGCCTTGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATg                                                                                                     >  3:369812/1‑150 (MQ=255)
                                                  tttCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTa                                                                                           >  3:192038/1‑150 (MQ=255)
                                                        ttttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc                                                                                     <  3:318756/150‑1 (MQ=255)
                                                        ttttGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGcc                                                                                     >  4:188501/1‑150 (MQ=255)
                                                           tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCgctggct                                                                                                                              >  1:409177/1‑106 (MQ=255)
                                                           tGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCgctggct                                                                                                                              <  2:409177/106‑1 (MQ=255)
                                                            gAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGc                                                                                 >  1:360105/1‑150 (MQ=255)
                                                                 cGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTaa                                                                            <  1:78439/150‑1 (MQ=255)
                                                                 cGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTaa                                                                            <  3:233238/150‑1 (MQ=255)
                                                                   gggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGc                                                                          >  3:279269/1‑150 (MQ=255)
                                                                    ggCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGcc                                                                         <  1:614979/150‑1 (MQ=255)
                                                                     gCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCg                                                                        <  1:398334/150‑1 (MQ=255)
                                                                         aTGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAAc                                                                    >  1:718827/1‑150 (MQ=255)
                                                                                   acgcTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc                                                          >  2:372726/4‑150 (MQ=255)
                                                                                   acacTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCAc                                                          <  3:188501/150‑1 (MQ=255)
                                                                                     acTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTa                                                        >  4:282317/1‑150 (MQ=255)
                                                                                     acTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTa                                                        >  3:245286/1‑150 (MQ=255)
                                                                                     acTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTa                                                        >  2:196610/1‑150 (MQ=255)
                                                                                       ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcgc                                                               >  1:109115/1‑141 (MQ=255)
                                                                                       ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCAcgc                                                               <  2:109115/141‑1 (MQ=255)
                                                                                       ttCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTAtt                                                      >  3:354050/1‑150 (MQ=255)
                                                                                          cgcgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGc                                                   <  2:770392/150‑1 (MQ=255)
                                                                                          cgcgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGc                                                   >  2:324732/1‑150 (MQ=255)
                                                                                            cgcgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCt                                                 >  2:312533/1‑150 (MQ=255)
                                                                                              cgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCgctgg                                                                                                                                <  1:230152/69‑1 (MQ=255)
                                                                                              cgACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCgctgg                                                                                                                                >  2:230152/1‑69 (MQ=255)
                                                                                               gACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGAGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAAAGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGCCCTGGt                                              >  4:397337/1‑150 (MQ=255)
                                                                                                  cAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTa                                           >  3:358031/1‑150 (MQ=255)
                                                                                                    gCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAACCGATTGGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATc                                         <  3:488725/150‑1 (MQ=255)
                                                                                                     ccTGGAAACTCTGGGCAAAATGGTCGCGGCAGGTCGCGGGATCAATTTACTGCTAGCGCTGGATGTGCCGCTGGAGCGCAAACGCGATGGGGTTGTTTATCTGCGGTGCATTAAGCCGGAAGCACGCCGCACTACTGGGGTGGTTTAttg                                        >  4:99915/1‑148 (MQ=255)
                                                                                                       tGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTc                                      >  4:7656/1‑150 (MQ=255)
                                                                                                              tctGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGTCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTc                               >  4:464695/1‑150 (MQ=255)
                                                                                                              tctGCGCAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTc                               >  2:617414/1‑150 (MQ=255)
                                                                                                                tGCGCAACATGGTGGCGGCAGGTAGCGGGATCAAtt                                                                                                                                               <  2:483547/36‑1 (MQ=255)
                                                                                                                    cAACATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCt                         >  4:257612/1‑150 (MQ=255)
                                                                                                                     aaCATGGTGGCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTg                        <  4:192038/150‑1 (MQ=255)
                                                                                                                      aCATGGTGGCGGCGGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGGGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCTTTCATTAAGCCGGAACCACGCCGCACTATTGGTCTGGTTTATCGTCCTGGCTCACCGCTgc                       <  2:666746/150‑1 (MQ=255)
                                                                                                                         tggtggCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCa                    >  4:247674/1‑150 (MQ=255)
                                                                                                                         tggtggCGGCAGGTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCa                    <  1:132136/150‑1 (MQ=255)
                                                                                                                                    ggTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTCCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGag         <  1:802316/150‑1 (MQ=255)
                                                                                                                                     gTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAg                   <  4:103441/139‑1 (MQ=255)
                                                                                                                                     gTAGCGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAg                   >  3:103441/1‑139 (MQ=255)
                                                                                                                                         cGGGATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCt    <  4:220334/150‑1 (MQ=255)
                                                                                                                                           ggATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCgg                                                                       >  2:247228/1‑81 (MQ=255)
                                                                                                                                           ggATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCgg                                                                       <  1:247228/81‑1 (MQ=255)
                                                                                                                                           ggATCAATTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTgg  >  2:13036/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCGCAAACGCGATGGGGTTGTTTATCTGCCGTGCATTAAGCCGGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTGG  >  NC_000913_3_hsa_tpiA/4159039‑4159327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑