BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	1,755,785	G→T	V30F (GTT→TTT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	1,755,785	0	G	T	92.5%	416.3 / 21.7	133	V30F (GTT→TTT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base G (4/6); new base T (62/61); total (66/67)
Fisher's exact test for biased strand distribution p-value = 7.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 134 total aligned reads displayed.

GGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAA  >  NC_000913_3_hsa_tpiA/1755638‑1755931

ggCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACttt                                                                                                                                                  >  4:576254/1‑150 (MQ=255)

  cAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTAt                                                                                                                                                >  1:966881/1‑150 (MQ=255)

   aGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATg                                                                                                                                               <  4:624866/150‑1 (MQ=255)

    gTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGc                                                                                                                                              <  3:685493/150‑1 (MQ=255)

       cgcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTc                                                                                                                                           <  2:6609/150‑1 (MQ=255)

        gcCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCt                                                                                                                                          >  2:981321/1‑150 (MQ=255)

         cccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTg                                                                                                                                         >  1:878014/1‑150 (MQ=255)

          ccAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGa                                                                                                                                        >  4:552932/1‑150 (MQ=255)

            aGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAAc                                                                                                                                      <  2:834272/150‑1 (MQ=255)

                aGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTtct                                                                                                                                  <  1:674864/150‑1 (MQ=255)

                        tctcCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGt                                                                                                                          <  1:1283600/150‑1 (MQ=255)

                           cccATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGAc                                                                                                                       >  3:156014/1‑150 (MQ=255)

                             cATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTa                                                                                                                     <  1:726154/150‑1 (MQ=255)

                                ccTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGc                                                                                                                  <  1:580181/150‑1 (MQ=255)

                                  ttCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAg                                                                                                                <  2:289282/150‑1 (MQ=255)

                                   tctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGa                                                                                                               >  2:675864/1‑150 (MQ=255)

                                    ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTtct                                                                                                                                  >  1:505080/1‑130 (MQ=255)

                                    ctcAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTtct                                                                                                                                  <  2:505080/130‑1 (MQ=255)

                                     tcAACTTAAAGACTAAGACTGTCATGAAAAAGACCGAAATTGTTTGCACCATCGGACCGAAAACCGAATCTTATGAGGTGTGAGCTAAAGTGCTTGTCGCGGTCATGAATTTGATTCGTCTGGACGTCTCTCATGGTGACTATGCAGAac                                                                                                             >  3:400124/1‑150 (MQ=255)

                                      cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAaca                                                                                                            >  2:306131/1‑150 (MQ=255)

                                      cAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAaca                                                                                                            >  4:290552/1‑150 (MQ=255)

                                       aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCt                                                                                                                                          >  2:1042012/1‑119 (MQ=255)

                                       aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCt                                                                                                                                          <  1:1042012/119‑1 (MQ=255)

                                       aaCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAacac                                                                                                           >  4:667953/1‑150 (MQ=255)

                                        aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTa                                                                                                                                                 >  4:230586/1‑111 (MQ=255)

                                        aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTa                                                                                                                                                 <  3:230586/111‑1 (MQ=255)

                                        aCTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACg                                                                                                          <  2:779092/150‑1 (MQ=255)

                                          ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGt                                                                                                        >  3:152708/1‑150 (MQ=255)

                                          ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGt                                                                                                        >  4:590887/1‑150 (MQ=255)

                                          ttAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGt                                                                                                        >  4:457607/1‑150 (MQ=255)

                                           tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTc                                                                                                       >  2:1236058/1‑150 (MQ=255)

                                           tAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTc                                                                                                       >  1:319867/1‑150 (MQ=255)

                                             aagactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAg                                                                                                     >  1:789043/1‑150 (MQ=255)

                                              agactaagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAgc                                                                                                    <  3:754138/150‑1 (MQ=255)

                                                   aagactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCAtt                                                                                               <  4:13329/150‑1 (MQ=255)

                                                    agactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTc                                                                                              <  3:803460/150‑1 (MQ=255)

                                                     gactGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCa                                                                                             <  3:759134/150‑1 (MQ=255)

                                                       ctGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGa                                                                                           <  4:377369/150‑1 (MQ=255)

                                                       ctGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGa                                                                                           <  2:79533/150‑1 (MQ=255)

                                                        tGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGaa                                                                                          <  3:576254/150‑1 (MQ=255)

                                                            aTGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGATCACGGTCAGCGCATTCAGAATCTg                                                                                      >  1:647936/1‑150 (MQ=255)

                                                             tGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                                                                                     >  4:439168/1‑150 (MQ=255)

                                                             tGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                                                                                     >  1:617500/1‑150 (MQ=255)

                                                             tGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTgc                                                                                     >  2:1201986/1‑150 (MQ=255)

                                                                 aaaGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCaa                                                                                 >  1:795904/1‑150 (MQ=255)

                                                                  aagtccAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCACGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAAc                                                                                <  1:564776/146‑1 (MQ=255)

                                                                     aCCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtg                                                                             <  2:492886/150‑1 (MQ=255)

                                                                       cAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgat                                                                           >  2:638417/1‑150 (MQ=255)

                                                                        aaaaTTGTTTGCACCATCGGACCGAAACCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtgatg                                                                          >  1:917527/1‑150 (MQ=255)

                                                                          aaTTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATg                                                                                                                   <  3:372117/107‑1 (MQ=255)

                                                                          aaTTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATg                                                                                                                   >  4:372117/1‑107 (MQ=255)

                                                                            ttGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCa                                                                      >  4:362226/1‑150 (MQ=255)

                                                                               tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa                                                                   <  1:1190823/150‑1 (MQ=255)

                                                                               tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa                                                                   <  2:597754/150‑1 (MQ=255)

                                                                               tttGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCaaaa                                                                   <  2:597647/150‑1 (MQ=255)

                                                                                  gCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTg                                                                <  4:802938/150‑1 (MQ=255)

                                                                                   cACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTgg                                                               <  1:1107893/150‑1 (MQ=255)

                                                                                       aTCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTaaa                                                           <  1:174248/150‑1 (MQ=255)

                                                                                             ccGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgcc                                                     >  4:249141/1‑150 (MQ=255)

                                                                                              cGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgccg                                                    >  1:95556/1‑150 (MQ=255)

                                                                                               gAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACcgccgc                                                   >  2:324826/1‑150 (MQ=255)

                                                                                                aaaaCCGAATCTGAAGAGATGTTAGCTAAAATGCTTGACGCTGGCATGAACTGTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCATAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCt                                                  >  4:522441/1‑150 (MQ=255)

                                                                                                aaaaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCt                                                  >  2:24113/1‑150 (MQ=255)

                                                                                                  aaCCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTAt                                                <  4:587087/150‑1 (MQ=255)

                                                                                                     cGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCt                                             >  1:282249/1‑150 (MQ=255)

                                                                                                        aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTg                                                                                                                                         <  3:431453/55‑1 (MQ=255)

                                                                                                        aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTg                                                                                                                                         >  4:431453/1‑55 (MQ=255)

                                                                                                        aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCTTTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCt                                          <  3:659541/150‑1 (MQ=255)

                                                                                                        aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCt                                          >  4:698114/1‑150 (MQ=255)

                                                                                                        aTCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCt                                          <  3:339830/150‑1 (MQ=255)

                                                                                                           tGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTCTGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAGCTGGTAAAACCGCCGCTATCCTGCTTGa                                       <  2:945820/150‑1 (MQ=255)

                                                                                                           tGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgcc                                                     <  3:755207/136‑1 (MQ=255)

                                                                                                           tGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAAccgcc                                                     >  4:755207/1‑136 (MQ=255)

                                                                                                             aaGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAAc                                                                  <  4:198469/121‑1 (MQ=255)

                                                                                                             aaGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATa                                     <  3:699416/150‑1 (MQ=255)

                                                                                                             aaGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATa                                     >  2:993983/1‑150 (MQ=255)

                                                                                                               gagaTGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATAcc                                   <  2:599055/150‑1 (MQ=255)

                                                                                                                  aTGTTAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCaaa                                <  1:24113/150‑1 (MQ=255)

                                                                                                                     ttAGCTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGtga                                                                            <  3:471681/103‑1 (MQ=255)

                                                                                                                         cTAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCgg                         <  1:40691/150‑1 (MQ=255)

                                                                                                                          tAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                      >  2:1118078/1‑136 (MQ=255)

                                                                                                                          tAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGAt                                      <  1:1118078/136‑1 (MQ=255)

                                                                                                                          tAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                        >  1:748367/1‑150 (MQ=255)

                                                                                                                          tAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                        >  3:211100/1‑150 (MQ=255)

                                                                                                                          tAAAATGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAAACCAACAAGACTCTGACCAACGTGATGCGCTAACCCCGTAAACCCGCCGCTATCCTGCTTGATACCAAAGGTCCGGa                        <  3:522441/150‑1 (MQ=255)

                                                                                                                            aaaTGCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGaaa                      >  4:647585/1‑150 (MQ=255)

                                                                                                                             aaTGCTGGACGCTGGCATGAACTTTATGCGTCTGaa                                                                                                                                       >  1:840534/1‑36 (MQ=255)

                                                                                                                             aaTGCTGGACGCTGGCATGAACTTTATGCGTCTGaa                                                                                                                                       <  2:840534/36‑1 (MQ=255)

                                                                                                                                gCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                  <  3:211622/150‑1 (MQ=255)

                                                                                                                                gCTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                  <  1:81201/150‑1 (MQ=255)

                                                                                                                                gCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCg                  <  4:646098/150‑1 (MQ=255)

                                                                                                                                 cTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGc                 >  1:750846/1‑150 (MQ=255)

                                                                                                                                 cTGGACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGc                 <  1:1236058/150‑1 (MQ=255)

                                                                                                                                  tGGAAGCTTGCATGGACTTTATGCGTCTGAACTGCGCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGAGGAGCAATACTGGGAAAACCGCCGCTATCCTGCTTGATAACAAAGGTCCGGAAATCCGCa                >  2:753765/1‑150 (MQ=255)

                                                                                                                                   ggACGCTGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCAc               <  1:1012749/150‑1 (MQ=255)

                                                                                                                                       gCTGGCACCAACGTTATGCCTCTGACCTCACCGGAGGTGGAGTAGGCACAACACGGTCAGCGCATTCAGAATCGGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCGAAGGTCCGGAAATCCGCACCATg           <  3:560574/150‑1 (MQ=255)

                                                                                                                                         tGGCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGaa         >  3:138413/1‑150 (MQ=255)

                                                                                                                                           gCATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGTATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAAc       >  4:792948/1‑150 (MQ=255)

                                                                                                                                            cATGAACTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACt      >  3:456681/1‑150 (MQ=255)

                                                                                                                                                aaCTTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGaa  >  4:472832/1‑150 (MQ=255)

GGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTTCTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCGCTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAA  >  NC_000913_3_hsa_tpiA/1755638‑1755931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`