Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 4,111,506 T→G intergenic (+821/+91) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA4,111,5060TG97.5% 415.5 / ‑1.5 118intergenic (+821/+91)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base G (60/55);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 118 total aligned reads displayed.

GCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAAC  >  NC_000913_3_hsa_tpiA/4111362‑4111650
                                                                                                                                                |                                                                                                                                                
gCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAg                                                                                                                                             >  1:1191730/1‑150 (MQ=255)
  acaAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCg                                                                                                                                           >  3:97752/1‑150 (MQ=255)
   caAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGa                                                                                                                                          >  2:1246422/1‑150 (MQ=255)
      accaccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATa                                                                                                                                       <  4:725350/150‑1 (MQ=255)
       ccaccTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATaa                                                                                                                                      >  4:64751/1‑150 (MQ=255)
           cTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGa                                                                                                                                  <  1:62864/150‑1 (MQ=255)
               gtgtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGAt                                                                                                                              >  2:236179/1‑150 (MQ=255)
                tgtCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATg                                                                                                                             >  1:624259/1‑150 (MQ=255)
                  tCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgc                                                                                                                           >  4:414957/1‑150 (MQ=255)
                   cagccggcACTTCCTCCGCGTTCAAAGAGACGATGAGCTCCCCCAGACACAGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAAAACTTCCTGGAGCGCCCCATCTTAATTCTCCACGCTGATAAGCGACTAAAGGTAGATGgcc                                                                                                                          >  4:775911/3‑150 (MQ=255)
                    ggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgccg                                                                                                                         >  2:1082057/1‑150 (MQ=255)
                    ggccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTGCCTGGAGGGCGCCATTTTAATGCTCCACGCTGATAAGCGAATAAAGGAAGATGgccg                                                                                                                         <  3:459414/150‑1 (MQ=255)
                      ccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgccgcc                                                                                                                       <  1:1205891/150‑1 (MQ=255)
                      ccggcACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGgccgcc                                                                                                                       >  4:533696/1‑150 (MQ=255)
                        gggcccTTGGCCGCTTTCAGAGTGCCGAGGCGCTCCCCCAGGCTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCCACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGcccc                                                                                                                     <  2:981138/146‑1 (MQ=255)
                            ccTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAg                                                                                                                 >  3:27714/1‑150 (MQ=255)
                             cTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAgg                                                                                                                >  4:540311/1‑150 (MQ=255)
                              ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               <  2:239664/150‑1 (MQ=255)
                              ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               <  4:259968/150‑1 (MQ=255)
                              ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               <  4:243823/150‑1 (MQ=255)
                              ttGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               >  4:349162/1‑150 (MQ=255)
                                ggCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgca                                                                                                             <  2:1248639/150‑1 (MQ=255)
                                ggCCGCGGTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgca                                                                                                             <  2:144111/150‑1 (MQ=255)
                                  ccGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagc                                                                                                           >  4:259835/1‑150 (MQ=255)
                                  ccGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagc                                                                                                           <  1:608320/150‑1 (MQ=255)
                                   cgcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGgcagca                                                                                                          >  2:74098/1‑150 (MQ=255)
                                    gcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGcagcag                                                                                                         >  4:41899/1‑150 (MQ=255)
                                    gcgTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGcagcag                                                                                                         >  4:41995/1‑150 (MQ=255)
                                         cAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAATGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtg                                                                                                    <  3:794863/150‑1 (MQ=255)
                                          agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                   <  3:533696/150‑1 (MQ=255)
                                          agagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                   <  1:246293/150‑1 (MQ=255)
                                          agagTGCCGATGAGCTCACCCAGCCTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgt                                                                                                   <  4:766960/150‑1 (MQ=255)
                                           gagTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCtgtg                                                                                                  <  1:842965/150‑1 (MQ=255)
                                                ccGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACa                                                                                             <  3:314627/150‑1 (MQ=255)
                                                  gATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATGTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGt                                                                                           <  4:292027/150‑1 (MQ=255)
                                                      aGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAACTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATag                                                                                       >  2:1223407/1‑150 (MQ=255)
                                                       gCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATaga                                                                                      <  1:331209/150‑1 (MQ=255)
                                                        cTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATagag                                                                                     >  4:728906/1‑150 (MQ=255)
                                                         tCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAgaga                                                                                    <  1:566058/150‑1 (MQ=255)
                                                          cccccAGACTCTGCTGCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGCAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAt                                                                                   <  2:791698/150‑1 (MQ=255)
                                                           ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAtt                                                                                  >  1:122305/1‑150 (MQ=255)
                                                           ccccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAtt                                                                                  >  1:363549/1‑150 (MQ=255)
                                                             ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCa                                                                                >  4:59880/1‑150 (MQ=255)
                                                             ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCa                                                                                >  4:330043/1‑150 (MQ=255)
                                                             ccAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCa                                                                                >  1:984865/1‑150 (MQ=255)
                                                                gACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGATCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCg                                                                             <  1:713580/150‑1 (MQ=255)
                                                                    ctGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGc                                                                                                                                            <  1:325022/83‑1 (MQ=255)
                                                                      gCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaa                                                                       >  1:711514/1‑150 (MQ=255)
                                                                       cTTCCGCCCGTTCATTTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                                                      <  3:29798/150‑1 (MQ=255)
                                                                       cTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGCATAAAGGAAGCTGGCCGCCCTGCAGCACAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACaaa                                                                      >  1:1081017/1‑150 (MQ=255)
                                                                          ccGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCAGTGAAACAGTATAGAGATTCATCGGCACAAAGgc                                                                   >  2:835096/1‑150 (MQ=255)
                                                                            gCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctt                                                                 >  3:533463/1‑150 (MQ=255)
                                                                             cccGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgcttt                                                                <  1:621012/150‑1 (MQ=255)
                                                                              ccGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               >  1:828489/1‑102 (MQ=255)
                                                                              ccGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAggg                                                                                                               <  2:828489/102‑1 (MQ=255)
                                                                               cGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgc                                                              >  4:740728/1‑150 (MQ=255)
                                                                                gTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgct                                                             >  2:567494/1‑150 (MQ=255)
                                                                                gTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgct                                                             >  1:356673/1‑150 (MQ=255)
                                                                                gTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGgctttgct                                                             >  1:1065514/1‑150 (MQ=255)
                                                                                            gTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTa                                                 <  3:349162/150‑1 (MQ=255)
                                                                                              ttCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTAtt                                               <  2:1191730/150‑1 (MQ=255)
                                                                                                cccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCa                                             <  2:277026/150‑1 (MQ=255)
                                                                                                cccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCa                                             <  4:214515/150‑1 (MQ=255)
                                                                                                 ccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCGGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                            <  3:87095/150‑1 (MQ=255)
                                                                                                 ccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                            <  3:568848/150‑1 (MQ=255)
                                                                                                 ccccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAGTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCaa                                            >  3:764196/1‑150 (MQ=255)
                                                                                                   ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGTCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAAc                                          >  1:392809/1‑150 (MQ=255)
                                                                                                   ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAt                                                                                   >  2:122994/1‑109 (MQ=255)
                                                                                                   ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAt                                                                                   <  1:122994/109‑1 (MQ=255)
                                                                                                   ccAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAAc                                          <  2:365010/150‑1 (MQ=255)
                                                                                                     aaCGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCt                                        >  2:1120865/1‑150 (MQ=255)
                                                                                                         aagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGATATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa                                    <  2:232886/150‑1 (MQ=255)
                                                                                                         aagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa                                    <  4:562176/150‑1 (MQ=255)
                                                                                                         aagaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCaa                                    <  1:1246422/150‑1 (MQ=255)
                                                                                                          agaaCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAg                                   <  2:291823/150‑1 (MQ=255)
                                                                                                             aCTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGa                                <  2:984865/150‑1 (MQ=255)
                                                                                                               ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAtt                              <  3:728906/150‑1 (MQ=255)
                                                                                                               ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAtt                              >  3:54445/1‑150 (MQ=255)
                                                                                                               ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGAtt                              >  4:633171/1‑150 (MQ=255)
                                                                                                               ttCCTGGAGGGCGCCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGAATCATCGGCACAAAGGCTTTGCTGTTTGTCATTTATTCAAACCTTCAAGCGAtt                              >  1:827082/1‑150 (MQ=255)
                                                                                                                   tGGAGGGCGCCATTTTAATTATCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCATGCGATTCAGa                          >  2:597536/1‑150 (MQ=255)
                                                                                                                          cgcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGcc                   >  1:104796/1‑150 (MQ=255)
                                                                                                                          cgcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGcc                   <  1:1177742/150‑1 (MQ=255)
                                                                                                                           gcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTGTAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCa                  >  2:691644/1‑150 (MQ=255)
                                                                                                                           gcCATTTTAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCa                  >  2:304949/1‑150 (MQ=255)
                                                                                                                                tttAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTa             >  3:572820/1‑150 (MQ=255)
                                                                                                                                  tAATTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAAt           <  1:304949/150‑1 (MQ=255)
                                                                                                                                    aTTCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCg         <  4:54445/150‑1 (MQ=255)
                                                                                                                                     ttCTCCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCgg        <  4:508637/150‑1 (MQ=255)
                                                                                                                                      tctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGt       <  2:1065514/150‑1 (MQ=255)
                                                                                                                                      tctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGt       <  3:49424/150‑1 (MQ=255)
                                                                                                                                       ctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACCGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCATATAGCGCCAGCTTAATCGGtt      <  2:392809/150‑1 (MQ=255)
                                                                                                                                       ctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGtt      >  4:751232/1‑150 (MQ=255)
                                                                                                                                       ctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGtt      >  1:1053997/1‑150 (MQ=255)
                                                                                                                                       ctcCACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGtt      >  1:986332/1‑150 (MQ=255)
                                                                                                                                        tcCACGCTGATCAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCCAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTc     <  4:776362/150‑1 (MQ=255)
                                                                                                                                          cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCaa   >  4:621420/1‑150 (MQ=255)
                                                                                                                                          cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCaa   <  4:26453/150‑1 (MQ=255)
                                                                                                                                          cACGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCaa   >  1:499393/1‑150 (MQ=255)
                                                                                                                                           aCGCTGATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAAc  >  3:763102/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCACAAACCACCTCGGTGTCGGCCGGCACCTTGGCCGCGTTCAGAGTGCCGATGAGCTCCCCCAGACTCTGCTTCCGCCCGTTCATCTTCCAGTTTCCCCCAACGAAGAACTTCCTGGAGGGCGCCATTTTAATTCTCCACGCTTATAAGCGAATAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAAC  >  NC_000913_3_hsa_tpiA/4111362‑4111650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: