Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_hsa_tpiA 1,158,559 2 bp→TC coding (691‑692/1434 nt) ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA1,158,5590CT95.7% 291.9 / 2.5 92Q231S (CAG→TCG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (50/38);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01
*NC_000913_3_hsa_tpiA1,158,5600AC94.6% 311.1 / 4.3 92Q231S (CAG→TCG) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base C (49/38);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.38e-01

TCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCG  >  NC_000913_3_hsa_tpiA/1158417‑1158700
                                                                                                                                              ||                                                                                                                                            
tcAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGtttt                                                                                                                                        >  1:701136/1‑150 (MQ=255)
tcAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGtttt                                                                                                                                        <  1:31132/150‑1 (MQ=255)
 cAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTc                                                                                                                                       >  2:68960/1‑150 (MQ=255)
      ggCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACgg                                                                                                                                  <  1:493096/150‑1 (MQ=255)
        ctgctTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCg                                                                                                                                >  1:1099142/1‑150 (MQ=255)
              aCCAGAACCCGGTAGTTGCGTTTGGCCTTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGGTTTCCACGGCGACATTc                                                                                                                          >  4:492130/1‑150 (MQ=255)
                cAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcg                                                                                                                        >  1:990079/1‑150 (MQ=255)
                cAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcg                                                                                                                        >  2:292110/1‑150 (MQ=255)
                   aaCCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGt                                                                                                                     >  2:916944/1‑150 (MQ=255)
                   aaCCCGGTAGTTGCGGTTGGCAATTACGGGTTTATCGAACGTTGCCTGGTACCGTTTGGTCTTCACCACATCTTGAACGTACCTTTACAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCAGGGCGACATTCCGCGt                                                                                                                     >  2:460613/1‑150 (MQ=255)
                      ccGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTtat                                                                                                                  >  2:1222576/1‑150 (MQ=255)
                        ggTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                >  4:470649/1‑150 (MQ=255)
                        ggTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTAGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                >  1:822504/1‑150 (MQ=255)
                                      gCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGa                                                                                                  >  1:1024539/1‑150 (MQ=255)
                                          ttACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGc                                                                                              >  3:571978/1‑150 (MQ=255)
                                           tACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCg                                                                                             <  3:586985/150‑1 (MQ=255)
                                           tACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCg                                                                                             >  4:800102/1‑150 (MQ=255)
                                            aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                            >  3:667231/1‑150 (MQ=255)
                                            aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                            <  4:23589/150‑1 (MQ=255)
                                            aCGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCgg                                                                                            <  2:202331/150‑1 (MQ=255)
                                             cGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCggg                                                                                           >  3:333577/1‑150 (MQ=255)
                                                tttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCGGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaa                                                                                        >  1:1037910/1‑150 (MQ=255)
                                                tttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGt                                                                                          <  3:587056/148‑1 (MQ=255)
                                                tttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGt                                                                                          >  4:587056/1‑148 (MQ=255)
                                                tttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaa                                                                                        >  2:880731/1‑150 (MQ=255)
                                                 ttCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTaaa                                                                                       <  1:1258507/150‑1 (MQ=255)
                                                  tCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTACGCGTTATATGGCGGGTGACCCGACTGCGGGTAAAc                                                                                      >  1:249133/1‑150 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                >  1:473374/1‑121 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                <  2:473374/121‑1 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                   <  1:290813/150‑1 (MQ=255)
                                                     tCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGt                                                                                   >  2:366555/1‑150 (MQ=255)
                                                      cGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTc                                                                                  >  3:617509/1‑150 (MQ=255)
                                                       gAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCt                                                                                 <  2:980939/150‑1 (MQ=255)
                                                           gTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcgc                                                                                                                       >  2:780184/1‑108 (MQ=255)
                                                           gTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcgc                                                                                                                       <  1:780184/108‑1 (MQ=255)
                                                           gTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                >  1:307143/1‑115 (MQ=255)
                                                           gTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTatat                                                                                                                <  2:307143/115‑1 (MQ=255)
                                                             tGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGc                                                                           <  2:1024539/150‑1 (MQ=255)
                                                                cTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTc                                                                        <  1:68960/150‑1 (MQ=255)
                                                                   gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTg                                                                     <  1:605930/150‑1 (MQ=255)
                                                                   gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTg                                                                     >  4:244814/1‑150 (MQ=255)
                                                                   gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTg                                                                     >  2:1017721/1‑150 (MQ=255)
                                                                   gTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTg                                                                     >  1:968361/1‑150 (MQ=255)
                                                                    tACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGt                                                                    <  3:732547/150‑1 (MQ=255)
                                                                     aCCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGtt                                                                   <  1:1203576/150‑1 (MQ=255)
                                                                     aCCGTGTGGACTGCACCGCATCGGGAACGTATCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGTTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGtt                                                                   <  2:238196/150‑1 (MQ=255)
                                                                       cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCa                                                                 <  3:250495/150‑1 (MQ=255)
                                                                       cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCa                                                                 >  4:652927/1‑150 (MQ=255)
                                                                       cGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGAGTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCa                                                                 <  2:72269/150‑1 (MQ=255)
                                                                        gTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaa                                                                >  3:627064/1‑150 (MQ=255)
                                                                         tttGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAGCGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaa                                                               <  2:883351/150‑1 (MQ=255)
                                                                          ttGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCaaaa                                                              <  2:1099142/150‑1 (MQ=255)
                                                                           tGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcgc                                                                                                                       >  1:659779/1‑92 (MQ=255)
                                                                           tGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCcgc                                                                                                                       <  2:659779/92‑1 (MQ=255)
                                                                              tCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTa                                                          <  3:390786/150‑1 (MQ=255)
                                                                                   accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACAt                                                                                                                            <  2:1067320/79‑1 (MQ=255)
                                                                                   accacATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACAt                                                                                                                            >  1:1067320/1‑79 (MQ=255)
                                                                                        aTCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCa                                                >  1:909157/1‑150 (MQ=255)
                                                                                         tCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCt                                                    <  1:264813/145‑1 (MQ=255)
                                                                                         tCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCt                                                    >  2:264813/1‑145 (MQ=255)
                                                                                            ggAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                            <  1:366555/150‑1 (MQ=255)
                                                                                                    ccTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAAtt                                    <  3:74741/150‑1 (MQ=255)
                                                                                                     cTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTg                                   >  3:59096/1‑150 (MQ=255)
                                                                                                         ccAGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTAt                               >  2:1259274/1‑150 (MQ=255)
                                                                                                           aGATGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCt                             >  4:84497/1‑150 (MQ=255)
                                                                                                             aTGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                           <  1:460613/150‑1 (MQ=255)
                                                                                                             aTGCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTgg                           <  2:417542/150‑1 (MQ=255)
                                                                                                               gCAGATTTGTGAATACACCAACGCAGCAGGTTCGGTTATCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGTCTGCGCGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                         <  2:1037934/150‑1 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGGGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                         >  2:725281/1‑150 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTTGTGGCTTCCTGTTCAAAATGTACCGACTGCCAGCTGCCGCAATTGCTATCTGGCa                         >  4:704520/1‑150 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACg                                                        >  3:648400/1‑119 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACg                                                        <  4:648400/119‑1 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCCGCTGCCGCAATTGCTATCTGGCa                         >  3:639579/1‑150 (MQ=255)
                                                                                                               gCAGATTGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCa                         >  3:593246/1‑150 (MQ=255)
                                                                                                                     tGGTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGc                   <  2:221423/150‑1 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggtg                                                                             <  4:223792/90‑1 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCtggtg                                                                             >  3:223792/1‑90 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                            >  2:188102/1‑123 (MQ=255)
                                                                                                                       gTGAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTg                                            <  1:188102/123‑1 (MQ=255)
                                                                                                                         gAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa               >  1:325603/1‑150 (MQ=255)
                                                                                                                         gAATACACCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTaaa               <  3:800102/150‑1 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGTCGACATTCCGCGTTATATGGCGCGTGACCCGACTGCCGGTAAACTGACTGGTGGCTTCCTGTTCACAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACCCTGCTAAACCAGaa         >  1:30163/1‑150 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCa            >  2:892644/1‑147 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCa            <  1:892644/147‑1 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa         >  2:147589/1‑150 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa         >  3:809211/1‑150 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa         <  1:531707/150‑1 (MQ=255)
                                                                                                                               acCAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaa         >  2:833432/1‑150 (MQ=255)
                                                                                                                                 cAACGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGaaaa       <  3:652927/150‑1 (MQ=255)
                                                                                                                                  aaCGCAGCAGGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAAc      >  1:534730/1‑150 (MQ=255)
                                                                                                                                      cagcagGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg  >  2:430890/1‑150 (MQ=255)
                                                                                                                                      cagcagGTTCGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACcgcg  <  1:54609/150‑1 (MQ=255)
                                                                                                                                              ||                                                                                                                                            
TCAGTGGGCTGCTTACCAGAACCCGGTAGTTGCGTTTGGCATTTACGGTTTCATCGAACGTTGCCTGGTACCGTTTGGTCTGCACCACATCTGGAACGTACCTTTCCAGATGCAGATTGGTGAATACACCAACGCAGCAGGTCAGGTTTTCCACGGCGACATTCCGCGTTATATGGCGGGTGACCCGACTGCGGGTAAACTGTCTGGTGGCTTCCTGTTCAAAATGTACGGTCTGCCAGCTGCCGCAATTGCTATCTGGCACTCTGCTAAACCAGAAAACCGCG  >  NC_000913_3_hsa_tpiA/1158417‑1158700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: