Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_hsa_tpiA	708,225	A→G	intergenic (+468/‑109)	glnS → / → chiP	glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_hsa_tpiA	708,225	0	A	G	100.0%	67.1 / NA	22	intergenic (+468/‑109)	glnS/chiP	glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/12);  total (10/12)

TGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGTTTAGTGGCAAACGTAGTACGCT  >  NC_000913_3_hsa_tpiA/708082‑708365                                                                                                                                                |                                                                                                                                             tGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTg                                                                                                                                         <  2:690506/149‑1 (MQ=255)    aaTTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTc                                                                                                                                      <  1:71118/149‑1 (MQ=255)              ttttATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAg                                                                                                                            <  1:558675/149‑1 (MQ=255)                  aTTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCgag                                                                                                                        >  1:570584/1‑149 (MQ=255)                          gCAAGCCTTTCAACCGCAAACTTAAGGCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGTGACTTTtct                                                                                                                >  2:604093/1‑149 (MQ=255)                              gCCTTTCAACCGCAAACTTAAGCCGTGTAACAAAAATAATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAAGTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAAc                                                                                                            <  2:312319/149‑1 (MQ=255)                                       ccGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACtttt                                                                                                   <  2:100542/149‑1 (MQ=255)                                                               aaaTCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTc                                                                           >  1:89652/1‑149 (MQ=255)                                                                        aaaTATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGt                                                                  >  2:551943/1‑149 (MQ=255)                                                                                gCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCGGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTAAATTAATCGCTGTTCACCTTTGGTGAAGCAAt                                                          >  1:9697/1‑149 (MQ=255)                                                                                         cATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTTGCGTCGCGACAAATGGTCTGTTCCTGTAGTTAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTc                                                 <  2:521490/149‑1 (MQ=255)                                                                                         cATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTc                                                 >  2:248626/1‑149 (MQ=255)                                                                                         cATGTACTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCTACGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCGCAACGCTACTTTTTTAATTTTTATTGTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTc                                                 <  2:152561/149‑1 (MQ=255)                                                                                                ttACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAgg                                          >  1:182590/1‑149 (MQ=255)                                                                                                 tACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGa                                         <  1:495297/149‑1 (MQ=255)                                                                                                     ttCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTaa                                     >  2:98372/1‑149 (MQ=255)                                                                                                                gAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCTGCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTAc                          <  1:144387/149‑1 (MQ=255)                                                                                                                gAAAGAGATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTAc                          <  1:248626/149‑1 (MQ=255)                                                                                                                   agacATTGATAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGtt                       <  2:726397/149‑1 (MQ=255)                                                                                                                            tAATTCGCGTCGCGAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGTTTAGTGGCaa              >  1:149598/1‑149 (MQ=255)                                                                                                                                 cgcgtcgcgAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGTTTAGTGGCAAACgta         <  2:389084/149‑1 (MQ=255)                                                                                                                                        cgAAAAATGGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGTTTAGTGGCAAACGTAGTAcgct  >  1:648472/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                             TGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGGTTGCTCATGTTCTTACATTCTGGTTACAGAAAGAGATTGATAATTCGCGTCGCGAAAAATAGTCTGTTCCTGTAGTCAGCGAGACTTTTCTCAACGCTACTTTTTTAATTTTTATTTTTTCGCTGTTCACCTTTGGTGCAGCAATTTATACGTCAAAGAGGATTAACCCATGCGTACGTTTAGTGGCAAACGTAGTACGCT  >  NC_000913_3_hsa_tpiA/708082‑708365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑