Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_hsa_tpiA2,751,9420GC22.2% 182.5 / 20.6 72A50P (GCT→CCT) recNrecombination and repair protein
Reads supporting (aligned to +/- strand):  ref base G (13/43);  new base C (16/0);  total (29/43)
Fisher's exact test for biased strand distribution p-value = 1.65e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAG  >  NC_000913_3_hsa_tpiA/2751795‑2752090
                                                                                                                                                   |                                                                                                                                                    
aTGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCt                                                                                                                                                    <  1:179640/150‑1 (MQ=255)
 tGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTg                                                                                                                                                   >  2:418509/1‑150 (MQ=255)
   ttGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGaa                                                                                                                                                 <  3:114778/150‑1 (MQ=255)
      gCACAACTGACCATCACCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTGACGcc                                                                                                                                              >  2:319057/1‑150 (MQ=255)
       cacaACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGacaccg                                                                                                                                             >  2:473994/1‑145 (MQ=255)
        acaACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGCTTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTGTAGCTGa                                                                                                                                            >  4:228644/1‑150 (MQ=255)
          aaCTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTAATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGGTGAAACCGACa                                                                                                                                          >  4:296676/1‑150 (MQ=255)
             tGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCAGACATgg                                                                                                                                       >  3:380794/1‑150 (MQ=255)
                   tCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTa                                                                                                                                 <  2:253470/150‑1 (MQ=255)
                     aGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTGACACCGACATGGTACGTAcc                                                                                                                               >  4:358810/1‑150 (MQ=255)
                      gCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCg                                                                                                                              >  1:456608/1‑150 (MQ=255)
                      gCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTGAAGCCAACAAGGAGAGTAccc                                                                                                                              >  2:324122/1‑149 (MQ=255)
                           tttGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTAACACCGACAAGATGCGTACCGGCgct                                                                                                                         >  2:168127/1‑150 (MQ=255)
                           tttGCTATCGTTCGTGAGCTTGAGATTGATTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCgctg                                                                                                                        <  1:65841/150‑1 (MQ=255)
                            ttGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCgctg                                                                                                                        <  2:342730/150‑1 (MQ=255)
                                tATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTcg                                                                                                                    <  1:560095/150‑1 (MQ=255)
                                 aTCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTcgc                                                                                                                   >  4:420746/1‑150 (MQ=255)
                                       cttgggCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGGTGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAc                                                                                                             <  3:219135/145‑1 (MQ=255)
                                          gAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtg                                                                                                          >  3:3196/1‑150 (MQ=255)
                                           aGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCtgt                                                                                                         <  3:40421/150‑1 (MQ=255)
                                             cTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTgc                                                                                                       >  3:71797/1‑150 (MQ=255)
                                                gagaTTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGcc                                                                                                    <  1:144059/150‑1 (MQ=255)
                                                 ggattgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGccc                                                                                                   <  4:177240/149‑1 (MQ=255)
                                                     tgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGttt                                                                                               <  1:254693/150‑1 (MQ=255)
                                                     tgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGttt                                                                                               <  1:244666/150‑1 (MQ=255)
                                                     tgattTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAACCTATTGCAATAGATGCCCTCGGTCTTTGTCGCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGttt                                                                                               <  1:504546/150‑1 (MQ=255)
                                                     tacttttCATAGATGAAAGTCCGTAATAAACGACGAGTGCGGAGAGGGTAAATCTATTTCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCAGTGCGCACGttt                                                                                               <  4:315126/147‑1 (MQ=255)
                                                        ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                                                                                            <  1:290459/150‑1 (MQ=255)
                                                        ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                                                                                            <  3:420746/150‑1 (MQ=255)
                                                        ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAATAAGTATCGGCGCTGCTCGCGCTGACCTGTGCGCCCGtttttt                                                                                            >  4:418111/1‑149 (MQ=255)
                                                        ttttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCCTATCATTAAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtc                                                                                            >  4:251297/1‑150 (MQ=255)
                                                         tttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtct                                                                                           <  3:138748/150‑1 (MQ=255)
                                                          ttCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTtctc                                                                                          <  1:214387/150‑1 (MQ=255)
                                                           tCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTctct                                                                                         <  4:3293/150‑1 (MQ=255)
                                                           tCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTGAAGCCGACAAAGAGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTctct                                                                                         >  2:13801/1‑150 (MQ=255)
                                                               aGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGaaa                                                                                     <  1:552497/150‑1 (MQ=255)
                                                               aGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTAACACTGACAAGAAGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGaaa                                                                                     >  2:280457/1‑150 (MQ=255)
                                                                 cGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGa                                                                                   <  3:381610/150‑1 (MQ=255)
                                                                  ggCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGAt                                                                                  <  1:293441/150‑1 (MQ=255)
                                                                         ccGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAg                                                                           <  1:428682/150‑1 (MQ=255)
                                                                           gTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCg                                                                         <  1:332773/150‑1 (MQ=255)
                                                                              ataaCTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGct                                                                      <  1:220387/150‑1 (MQ=255)
                                                                                  cTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTgc                                                                  <  2:361170/150‑1 (MQ=255)
                                                                                  cTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTgc                                                                  >  3:269672/1‑150 (MQ=255)
                                                                                     gagagaCCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgct                                                               <  4:194471/148‑1 (MQ=255)
                                                                                     gCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAACAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgct                                                               >  4:215347/1‑150 (MQ=255)
                                                                                      cGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgctg                                                              <  3:164914/150‑1 (MQ=255)
                                                                                        ggcccGGCGCGGGTAAATCTATTGCGATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCgctggc                                                            <  2:163812/147‑1 (MQ=255)
                                                                                             ggCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGgaa                                                       <  1:46794/150‑1 (MQ=255)
                                                                                               cgcgGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGgaaga                                                     <  2:456608/150‑1 (MQ=255)
                                                                                                 cgGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGa                                                                                                                                                  >  1:117239/1‑55 (MQ=255)
                                                                                                 cgGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGa                                                                                                                                                  <  2:117239/55‑1 (MQ=255)
                                                                                                  gggTAAATCTATGGGAAAAGATGCGCTCGGTCGTCGTCTCGGTGGTTGCGGTGAAGCCGACATAGTGCGTACCTGCGCTGCTCGCGCTGACCGGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGaaaa                                                  <  4:145387/150‑1 (MQ=255)
                                                                                                   ggTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAAc                                                 <  3:97168/150‑1 (MQ=255)
                                                                                                     tAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCa                                               <  1:225847/150‑1 (MQ=255)
                                                                                                      aaaTCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAg                                              <  1:52967/150‑1 (MQ=255)
                                                                                                      aaaTCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTAACATCAAAATAGTACGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGTTCTGCGCTGGCTGGAAGAAAACCAg                                              >  2:400967/1‑150 (MQ=255)
                                                                                                         tCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCtt                                           >  2:585195/1‑150 (MQ=255)
                                                                                                           tATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTAGCCGTAACGACAAAATAGCACATACTGGCCACACTCGAAAAAAACTATGAACCAGATTTCATCTGAAAGATACGCGAGCAGCTCTGCGACGGCCGGGAGAAAACAAGCTTgc                                         >  2:92467/1‑149 (MQ=255)
                                                                                                            aTTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAATAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGACGAAAACCAGCTTGaa                                        >  2:172456/1‑150 (MQ=255)
                                                                                                             ttGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAg                                       >  3:290094/1‑150 (MQ=255)
                                                                                                              tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAc                                                                                                             <  2:494304/79‑1 (MQ=255)
                                                                                                              tGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGAc                                                                                                             >  1:494304/1‑79 (MQ=255)
                                                                                                               gCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTCAAAGATACGCCAGCGGCTCTACGCTGGCTGGAAGAAAACCAGCTTGAAGAc                                     >  4:345008/1‑150 (MQ=255)
                                                                                                                 aaTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACgg                                   <  2:187925/150‑1 (MQ=255)
                                                                                                                 aaTAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCCGTATCATCAAAAAAAAACGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACgg                                   >  4:407072/1‑150 (MQ=255)
                                                                                                                    agctGCCCTCGGTCTTTGTCTCGGTGGTCGCGATGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCCGCCCTATGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCa                                <  1:639462/147‑1 (MQ=255)
                                                                                                                         cccTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAAt                           <  1:210678/150‑1 (MQ=255)
                                                                                                                                 cTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCtt                   <  2:204122/150‑1 (MQ=255)
                                                                                                                                     gTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTtcgtc               <  3:110507/150‑1 (MQ=255)
                                                                                                                                            gggtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGAt        <  4:238873/149‑1 (MQ=255)
                                                                                                                                              gtCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATca      >  3:224568/1‑150 (MQ=255)
                                                                                                                                                 gcgcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATcagca   <  1:13801/150‑1 (MQ=255)
                                                                                                                                                  cgcTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATcagcag  <  4:307490/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAGCGGCATGACCGTAATAACTGGCGAGACCGGCGCGGGTAAATCTATTGCAATAGATGCCCTCGGTCTTTGTCTCGGTGGTCGCGCTGAAGCCGACATGGTGCGTACCGGCGCTGCTCGCGCTGACCTGTGCGCCCGTTTTTCTCTGAAAGATACGCCAGCGGCTCTGCGCTGGCTGGAAGAAAACCAGCTTGAAGACGGGCATGAATGTTTGCTTCGTCGCGTGATCAGCAG  >  NC_000913_3_hsa_tpiA/2751795‑2752090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: