BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,363	Δ2 bp	intergenic (‑1/+1)	gatC ← / ← gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,361	0	C	.	98.1%	456.3 / 2.5	105	pseudogene (2/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand): ref base C (2/0); new base . (43/60); total (45/60)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01
*	NC_000913	2,173,362	0	C	.	98.1%	455.9 / 2.0	105	pseudogene (1/442 nt)	gatC	pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand): ref base C (2/0); new base . (43/60); total (45/60)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT  >  NC_000913/2173214‑2173502

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ggAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccacc                                                                                                                                              <  1:423630/149‑1 (MQ=255)

 gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTTCCCGGCACACAAACAGCAATTAAAATGCTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccg                                                                                                                                             >  2:8494/1‑148 (MQ=255)

 gAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccg                                                                                                                                             >  2:738441/1‑148 (MQ=255)

  aaCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTCATTACCCGGCACACCAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgc                                                                                                                                            >  1:1435563/1‑147 (MQ=255)

   accgATTTCCACGATGCACGGCGACCGCCATCTCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                           <  1:1141518/146‑4 (MQ=255)

   aCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                           <  1:1383047/149‑4 (MQ=255)

   aCAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                           <  2:1602778/149‑4 (MQ=255)

    cAGCTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccg                                                                                                                                          <  2:1320891/149‑5 (MQ=255)

    cAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccg                                                                                                                                          <  2:1327415/149‑5 (MQ=255)

     aGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGAGTGGGATAAACATCAGGCTTGCCGATaccaccgccgt                                                                                                                                         >  1:1603334/1‑144 (MQ=255)

      gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGTCAAGATCGCCAAACGCCAGCACCTGATTACCCGGCACACAACCAGCATTAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgta                                                                                                                                        <  2:822604/149‑7 (MQ=255)

       aTTTCCACTATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGGAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat                                                                                                                                       <  1:753584/149‑8 (MQ=255)

       aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAACCAGCAATTAAAATGGTGATGGGGATAAAAATCAGGCTTGCCGCTaccaccgccgtat                                                                                                                                       >  2:389010/1‑142 (MQ=255)

       aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat                                                                                                                                       <  2:146232/149‑8 (MQ=255)

       aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgccgtat                                                                                                                                       >  2:704490/1‑142 (MQ=255)

             aCGATGCACGGCGACCGCCATCGCTACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca                                                                                                                               <  1:331961/149‑1 (MQ=255)

             aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca                                                                                                                               <  1:1360966/149‑1 (MQ=255)

             aCGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCca                                                                                                                               <  2:334173/149‑1 (MQ=255)

               gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCCAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAAGGGGGAGTGGGATAAAAATCAGGCTTGACGAGACCA‑‑CCGCCGTAGGTCCcagc                                                                                                                             >  1:1099508/1‑149 (MQ=255)

                aTGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                            >  1:1544080/1‑149 (MQ=255)

                 tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                                                                                                                           >  1:779642/1‑149 (MQ=255)

                    aCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCaa                                                                                                                        >  1:1684324/1‑149 (MQ=255)

                        cGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACCGCAATTAAAATGGTGAGTGGGATAAAACTCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                                                                                                                    >  2:294925/1‑149 (MQ=255)

                         gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCg                                                                                                                   <  1:266524/149‑1 (MQ=255)

                          aCCGCCATCGCCAGGATGAAGCCGATGGTGGCAAGAGCGCCAAACGGCAGCAACTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAACATCAGGCGTGCCGATACCA‑‑CCGCCGTAAGTCCCAGCAGCAACGCCgg                                                                                                                  <  2:1157852/149‑1 (MQ=255)

                           ccGCCATCGCCACGACGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAAAAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                 <  2:1578196/149‑1 (MQ=255)

                           ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGGATACCCGGCACACAAAGAGCAATTAAAAGGGTGAGGGGGATAAATATCAGGCTTGCGGAGAGCA‑‑CCGCCGGATGTCCGAGCAGCCACGCCggt                                                                                                                 >  2:1049294/1‑148 (MQ=255)

                           ccGACATCGCACAGAAGAAGCCGATGGTGGCAAGATCGACAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGTTGAGTGTGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAAGCCGGa                                                                                                                 <  2:1516640/149‑1 (MQ=255)

                            cGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt                                                                                                                >  1:1474254/1‑149 (MQ=255)

                                aTCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAg                                                                                                            <  2:728157/149‑1 (MQ=255)

                                  cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                          <  1:1278476/149‑1 (MQ=255)

                                    ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                        >  2:1614317/1‑149 (MQ=255)

                                      aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                      <  2:1474254/149‑1 (MQ=255)

                                      aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGAGAGTGGGATAAAAATCAGGCTTGCGGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGACGGATCAAGGCCAAt                                                                                                      >  2:828220/1‑149 (MQ=255)

                                      aCGAAGAAGCCGAAGGTGGCAATATCGCCAAACTGCAGCACCTGATTACCCGGCACACAAACTGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                      <  1:1455946/149‑1 (MQ=255)

                                        gaataaGCCGATGGTGGCAAGATCGCCAAACTGCAGCACCTGAATACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                    <  1:1570949/149‑1 (MQ=255)

                                        gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCAGCTGATTACCCGGCACACAAACAGCAATTAATATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCCTATGTCCCCCCAGCAACGCCGGATCACGGCCTATCa                                                                                                    >  1:801755/1‑149 (MQ=255)

                                        gaagaaGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                    <  1:738441/149‑1 (MQ=255)

                                        gaagaaACCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGAATACCCGGCACACAAACAGCAATAAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCCATACCA‑‑CAGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                                    >  1:1173108/1‑149 (MQ=255)

                                              gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACt                                                                                              >  1:1371071/1‑149 (MQ=255)

                                               ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                             >  2:887898/1‑149 (MQ=255)

                                                 gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                           <  1:528038/149‑1 (MQ=255)

                                                   tggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGTATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGa                                                                                         <  2:1111167/149‑1 (MQ=255)

                                                        gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGCACTCCTGAccgcc                                                                                    >  1:1746302/1‑149 (MQ=255)

                                                               cGCCAAACGGCAGCACCTGATTCCCCGGCACCCAAACAGCAATTAAAATGGTGAGGGGGATCAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                             <  1:114853/149‑1 (MQ=255)

                                                                gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACATCg                                                                            >  2:1383251/1‑149 (MQ=255)

                                                                 ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                           <  2:726894/149‑1 (MQ=255)

                                                                 ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                           <  2:92304/149‑1 (MQ=255)

                                                                 ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGc                                                                           <  1:44600/149‑1 (MQ=255)

                                                                 ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGAAATTAAAATGGTGAGTGGGTTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCTTCAGCAACGCCGGATCAAGGCAAATCAGGAACTCATGACCGCCGAACTTCGc                                                                           >  1:941438/1‑149 (MQ=255)

                                                                   aaaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGCATAAAAATCAGGATTGCCGATACCACCTCG‑‑GTATGTCACAGCAGCAAAGCAGTATCAAGGCCAATCAGGAACTCCTGACCGCCTAACTTCGCCt                                                                         >  2:293708/1‑149 (MQ=255)

                                                                         cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc                                                                          <  2:1101774/142‑1 (MQ=255)

                                                                         cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc                                                                          >  1:1101774/1‑142 (MQ=255)

                                                                         cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                                   >  1:92695/1‑149 (MQ=255)

                                                                           gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGa                                                                 <  2:16157/149‑1 (MQ=255)

                                                                            caCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc                                                                >  2:207306/1‑149 (MQ=255)

                                                                              ccTGATTACCCGGCTCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCCGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGACCTTCGCCTGTAAACGACtc                                                              >  2:1321210/1‑148 (MQ=255)

                                                                              ccTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                              >  2:853295/1‑149 (MQ=255)

                                                                              ccTGATTACCCGACACACAAACAGCAATTAAAAGGGGGATTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAACAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                              <  1:1168197/149‑1 (MQ=255)

                                                                               cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTAc                                                             >  1:836287/1‑149 (MQ=255)

                                                                               cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTAc                                                             >  1:641645/1‑149 (MQ=255)

                                                                               cTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCATGTAAACGCCTAc                                                             >  2:1396082/1‑149 (MQ=255)

                                                                                 gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGa                                                           <  1:1614317/149‑1 (MQ=255)

                                                                                   ttACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                         <  1:1030106/149‑1 (MQ=255)

                                                                                       ccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                                     >  2:453109/1‑149 (MQ=255)

                                                                                        cGGCACACAAACAGCAATTAAAATGGTGAGTTGGATAAAAATCAGGCTTGCCGATACAA‑‑CCGCCGTATGTCCCAGCAGAAACGCCGGATCACGGCCAATCAGGCACTCATGACAGACGAACTTCGCCTGTAAACGACTACGAGccagat                                                    >  2:205939/1‑145 (MQ=255)

                                                                                        cGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGTCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCt                                                    >  1:146029/1‑149 (MQ=255)

                                                                                          gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                      >  1:1588966/1‑97 (MQ=255)

                                                                                          gCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                      <  2:1588966/97‑1 (MQ=255)

                                                                                              acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTGCTGACCGCCGAACTTCGCCGGTAGACGCCGACGCGCCGGCGTAGCGa                                              >  1:1661263/1‑149 (MQ=255)

                                                                                              acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGa                                              >  1:601247/1‑149 (MQ=255)

                                                                                                 aaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                           <  2:1544080/149‑1 (MQ=255)

                                                                                                    aGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACGTCGCCTGTAAACTAATACGAGACTGCTTAGCGATGGGCg                                        >  2:1276000/1‑149 (MQ=255)

                                                                                                        ataaaaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaa                                    <  2:1000736/146‑1 (MQ=255)

                                                                                                         ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCGTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                                   <  1:816471/149‑1 (MQ=255)

                                                                                                            aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGATCTCCTGACCGCAGAACTTCGAATTTTATCGACTACGAGCCTGCTTAGCGATGGGAGTGAAAcca                                >  2:1552098/1‑149 (MQ=255)

                                                                                                            aaaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                >  1:1254109/1‑149 (MQ=255)

                                                                                                             aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                               >  2:723136/1‑149 (MQ=255)

                                                                                                             aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                               >  2:1760722/1‑149 (MQ=255)

                                                                                                              aTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAacatc                              <  2:1435563/149‑1 (MQ=255)

                                                                                                                ggTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcca                            <  1:513337/149‑1 (MQ=255)

                                                                                                                    aGTGGGAAAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAACCCATCCatgat                        <  2:105144/149‑1 (MQ=255)

                                                                                                                     gTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgatg                       <  1:421794/149‑1 (MQ=255)

                                                                                                                      tggtaaaaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCAGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                      <  1:1716713/143‑1 (MQ=255)

                                                                                                                      tGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                      <  1:1094994/149‑1 (MQ=255)

                                                                                                                        ggATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGt                    <  1:704490/149‑1 (MQ=255)

                                                                                                                         gATTAACATCAGGCTTGCCGATTCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCATAGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                   <  1:251391/149‑1 (MQ=255)

                                                                                                                         gATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGtt                   >  2:888166/1‑149 (MQ=255)

                                                                                                                          aTAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt                  >  1:405182/1‑149 (MQ=255)

                                                                                                                            aaaaaTCAGGCTTGCCGATACCA‑‑CCGCCGTAGGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTaa                <  1:294925/149‑1 (MQ=255)

                                                                                                                             aaacTCAGGCTTGCCGATACCA‑‑GCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACGGGCGAACTTCGCCTGTAAACGACTACGTGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt               <  2:801755/149‑1 (MQ=255)

                                                                                                                              aaaTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGACGAACTGCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATc              <  1:207306/149‑1 (MQ=255)

                                                                                                                                   aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCCGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACAACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCg         <  1:1049294/149‑1 (MQ=255)

                                                                                                                                    gccTTGCCTATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGt        <  2:1432419/147‑1 (MQ=255)

                                                                                                                                       ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc     <  1:1383251/149‑1 (MQ=255)

                                                                                                                                       ttGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGACGTATCAAGGCCAATAAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc     <  2:1684324/149‑1 (MQ=255)

                                                                                                                                       atGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGc     <  1:276358/148‑1 (MQ=255)

                                                                                                                                         gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAt   <  2:1371071/149‑1 (MQ=255)

                                                                                                                                          ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt  <  1:389010/149‑1 (MQ=255)

                                                                                                                                          ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt  <  2:92695/149‑1 (MQ=255)

||

GGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATT  >  NC_000913/2173214‑2173502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`