Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,183,214	C→T	T657I (ACC→ATC)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,183,214	0	C	T	100.0%	293.7 / NA	88	T657I (ACC→ATC)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/43);  total (45/43)

ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGA  >  NC_000913/4183069‑4183358                                                                                                                                                  |                                                                                                                                                 aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACCTGGACGTAtccatcca                                                                                                                                               >  2:142845/1‑149 (MQ=255) aTCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTAtccatcca                                                                                                                                               >  2:137107/1‑149 (MQ=255)    gaagaaGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTACCAAAGGCGAATCAAGTCTGTTCACCCGCGACCAGGTTGACTACATGGCCGTATCCATccacca                                                                                                                                            >  2:618367/1‑149 (MQ=255)     aagaagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGCCGTATCCATCcagcag                                                                                                                                           >  1:643649/1‑149 (MQ=255)        aagGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAggtg                                                                                                                                        <  1:476464/149‑1 (MQ=255)              aCTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTGTCCATCCAGCAGGTGGTATcc                                                                                                                                  >  2:8678/1‑149 (MQ=255)               cTACGTTATCGCCCAGGCGAACTCTTTCTCGGAGGAGGCAGGTCACGATATAAAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCg                                                                                                                                 <  1:742190/149‑1 (MQ=255)               cTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCg                                                                                                                                 >  2:572358/1‑149 (MQ=255)                tACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCCGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGt                                                                                                                                >  1:25122/1‑149 (MQ=255)                  cGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCg                                                                                                                              <  2:598458/149‑1 (MQ=255)                    ttATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCGGCAGGTGGTATCCGTCGGt                                                                                                                            <  2:801713/149‑1 (MQ=255)                           ccAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTccc                                                                                                                     >  1:482802/1‑149 (MQ=255)                                    cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCcagca                                                                                                                                            >  1:113623/1‑117 (MQ=255)                                    cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCcagca                                                                                                                                            <  2:113623/117‑1 (MQ=255)                                    cTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGt                                                                                                            >  1:955947/1‑149 (MQ=255)                                     tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                                                                                           >  2:578495/1‑149 (MQ=255)                                     tCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGtt                                                                                                           <  1:474745/149‑1 (MQ=255)                                       cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTcc                                                                                                         >  1:765998/1‑149 (MQ=255)                                       cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTcc                                                                                                         >  1:269309/1‑149 (MQ=255)                                       cAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGAAAAGGCGAATCCAGCTTGTTCAGCCGCGACCCGGTTGACTACATGGACGTATCCATCCACCAGGTCGTATCCGTCGGTGCGTCCCTGCTCCCGTTct                                                                                                         >  2:405735/1‑148 (MQ=255)                                         aCTTGGATGAAGAAGGCCACTTCTTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                                       <  1:313972/149‑1 (MQ=255)                                         aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGTCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                                       <  2:895746/149‑1 (MQ=255)                                         aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTg                                                                                                       <  1:619536/149‑1 (MQ=255)                                         aCTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCAAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGCTCCTg                                                                                                       >  1:118970/1‑149 (MQ=255)                                          cTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTgc                                                                                                      >  2:969384/1‑148 (MQ=255)                                           ttGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGa                                                                                                     >  2:283555/1‑149 (MQ=255)                                            tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGaa                                                                                                    <  1:283555/149‑1 (MQ=255)                                            tGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGaa                                                                                                    <  1:82033/149‑1 (MQ=255)                                                tGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACg                                                                                                >  1:445200/1‑149 (MQ=255)                                                 gaagaaGGCCACTTCGTATAAGACCTGGTAACTGGCCGGAGCAAAGGCGAATCCTGCTTGTTCAGCCGTGACCAGGTCGGCTGTCTTCACGTTTTCATGCAGCATGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGa                                                                                               <  2:116695/149‑1 (MQ=255)                                                  aagaagGGCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGAt                                                                                              <  2:277595/149‑1 (MQ=255)                                                   agaagGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATg                                                                                             >  2:903921/1‑149 (MQ=255)                                                     gatgCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGATTGACTACATGGAGGTAGCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTGCCTGGAACACGATGAc                                                                                           >  2:937217/4‑149 (MQ=255)                                                     aagGCCACTTCGTAGAAGCCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGAc                                                                                           <  1:566994/149‑1 (MQ=255)                                                     aagGCCACTTCGTAGAAGACCAGGTAACTTGCCGTAGCAAAGGCGACTCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGGGGTATCCGTCGGTGCGCCCCTGAGCCCGTTCCTGGAACACGATGAc                                                                                           <  1:269654/149‑1 (MQ=255)                                                       gtccACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                         >  1:686680/3‑149 (MQ=255)                                                       ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTa                                                                                                                                     <  2:656365/105‑1 (MQ=255)                                                       ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTa                                                                                                                                     >  1:656365/1‑105 (MQ=255)                                                       ggCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGc                                                                                         >  2:852251/1‑149 (MQ=255)                                                         ccACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCa                                                                                       >  1:972290/1‑149 (MQ=255)                                                                  agaagaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCAt                                                                              <  1:655548/149‑1 (MQ=255)                                                                     agaCCTGGTAACTTGCCGTAGCAAAGGCTAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                           <  1:106946/149‑1 (MQ=255)                                                                     agaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGa                                                                           <  1:509273/149‑1 (MQ=255)                                                                      gaCCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGAt                                                                          <  2:269309/149‑1 (MQ=255)                                                                         cTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATggg                                                                       >  2:888470/1‑149 (MQ=255)                                                                          tGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGt                                                                      >  1:526478/1‑149 (MQ=255)                                                                            gTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGc                                                                    >  1:59411/1‑149 (MQ=255)                                                                               aCTGGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGCTGGCGCGGTAGCTGCATTGATGGGttggaa                                                                 <  2:482802/149‑6 (MQ=255)                                                                                 ttGCCGTAGCAAAGGCGAATCCAGCTTGATAAGCCGCGACCGGGTTGACTACATGGACGTATCCATCAAGCAGGTGGTATCCTTCGGTGCGTCGATGATCCTGTTCCTGGAAAACGATGAGGCCAACCGTGGATTGGTGGGTGcatgcc                                                               >  2:34617/1‑144 (MQ=255)                                                                                     cGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCa                                                           <  2:526478/149‑1 (MQ=255)                                                                                      gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                          <  1:568507/149‑1 (MQ=255)                                                                                      gTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCaa                                                          >  1:177162/1‑149 (MQ=255)                                                                                         gCAAAGGCGAATCCAGCTTGTTCAGGCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                       <  2:696402/149‑1 (MQ=255)                                                                                         gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGt                                                                                                                                <  1:908492/76‑1 (MQ=255)                                                                                         gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGt                                                                                                                                >  2:908492/1‑76 (MQ=255)                                                                                         gCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGt                                                       <  1:238766/149‑1 (MQ=255)                                                                                          cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAggtg                                                                                                                                        <  1:930158/67‑1 (MQ=255)                                                                                          cAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAggtg                                                                                                                                        >  2:930158/1‑67 (MQ=255)                                                                                           aaaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCa                                                     <  1:716188/149‑1 (MQ=255)                                                                                            aaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                    <  2:445200/149‑1 (MQ=255)                                                                                            aaGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                    >  2:516785/1‑149 (MQ=255)                                                                                            aaGGCCAATCCAGCTTGGTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAg                                                    <  2:575034/149‑1 (MQ=255)                                                                                               gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGcc                                                 >  2:428959/1‑149 (MQ=255)                                                                                               gCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGACACCATGCAACGTCAGGcc                                                 >  2:65881/1‑149 (MQ=255)                                                                                                 gAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGCACATGCAACGTCAGGCCGt                                               >  2:274940/1‑149 (MQ=255)                                                                                                          ttGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCCGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGActct                                      <  1:8678/149‑1 (MQ=255)                                                                                                             ttCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcg                                   <  1:59025/149‑1 (MQ=255)                                                                                                              tCAGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgcgc                                  <  1:618367/149‑1 (MQ=255)                                                                                                                aGCCGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTgtgcgc                                >  1:458850/1‑149 (MQ=255)                                                                                                                  ccGCGACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTg                              >  1:921156/1‑149 (MQ=255)                                                                                                                   cgggACCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTAGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGAGTCAGCGAGCTgt                             <  2:636166/146‑2 (MQ=255)                                                                                                                       aCCAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAg                         >  2:536819/1‑149 (MQ=255)                                                                                                                         cAGGTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATGAGcc                       >  1:265709/1‑149 (MQ=255)                                                                                                                            gTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTg                                                                                 >  1:691909/1‑88 (MQ=255)                                                                                                                            gTTGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTg                                                                                 <  2:691909/88‑1 (MQ=255)                                                                                                                             ttGACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtg                   >  1:273996/1‑149 (MQ=255)                                                                                                                               gACTACATGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGCCAAGCCGCTCCCACTCTCCGCGCTGATAAGCCGCcggt                 >  2:128363/1‑149 (MQ=255)                                                                                                                               gACTACATGGACGTATCCATCCAGCAGGTGGTACCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggt                 <  1:185759/149‑1 (MQ=255)                                                                                                                                 cTACATGGACGTAGCCATCCAGCAGGCGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttg               <  1:959022/149‑1 (MQ=255)                                                                                                                                     aTGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCtggttggtcc           >  1:125991/1‑147 (MQ=255)                                                                                                                                     aTGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc           <  1:683178/149‑1 (MQ=255)                                                                                                                                     aTGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCCGGAACACGATGACGCCAACCGTGCATCGAAAGGAGCCAACAAGCAACGTCAGGCCGTTCCGACTCTCCGCGCTGGTAGGCCGCgggttggtgc           >  2:957871/1‑147 (MQ=255)                                                                                                                                     aTGGACGTATCCATCCAGCAGATGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc           >  2:893004/1‑149 (MQ=255)                                                                                                                                     aTGGACGTATCCATCCAGCAGATGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTAc           >  2:892993/1‑149 (MQ=255)                                                                                                                                      tGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                                                                            >  1:368786/1‑67 (MQ=255)                                                                                                                                      tGGACGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGa                                                                                            <  2:368786/67‑1 (MQ=255)                                                                                                                                         aCGTATTCATCGAGCAGGGGGTATCCCTCGGTGCGGCCCCGATCCCGTTCCTGGAACACGATGACGGCAACCGTGCACTGATGGGTGCGAACATGCAACGTCTGGCCGTTCCGCCTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGt       <  2:205434/149‑1 (MQ=255)                                                                                                                                         aCGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGTGCGCTGATAAGCCGCTGGTTGGTACTGGt       <  2:458850/149‑1 (MQ=255)                                                                                                                                          cGTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTa      <  1:536819/149‑1 (MQ=255)                                                                                                                                           gTATCCATCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTAt     <  1:852251/149‑1 (MQ=255)                                                                                                                                             atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTCCTGGTATgg   >  1:358085/1‑149 (MQ=255)                                                                                                                                             atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATgg   >  2:772840/1‑149 (MQ=255)                                                                                                                                             atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATgg   >  1:319240/1‑149 (MQ=255)                                                                                                                                             atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATg    <  2:860955/148‑1 (MQ=255)                                                                                                                                             atccatccAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATg    >  1:860955/1‑148 (MQ=255)                                                                                                                                              tccatccaGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACATCAAGCCGTTCCGACTCTGCGCGCTGATAAGCCGCAGGTTGGTAATGGTATGGa  >  1:609969/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 ATCGAAGAAGGCAACTACGTTATCGCCCAGGCGAACTCCAACTTGGATGAAGAAGGCCACTTCGTAGAAGACCTGGTAACTTGCCGTAGCAAAGGCGAATCCAGCTTGTTCAGCCGCGACCAGGTTGACTACATGGACGTATCCACCCAGCAGGTGGTATCCGTCGGTGCGTCCCTGATCCCGTTCCTGGAACACGATGACGCCAACCGTGCATTGATGGGTGCGAACATGCAACGTCAGGCCGTTCCGACTCTGCGCGCTGATAAGCCGCTGGTTGGTACTGGTATGGA  >  NC_000913/4183069‑4183358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑