| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,270,083 | 0 | A | G | 36.0% | 17.5 / 19.9 | 27 | intergenic (+481/+133) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
| Reads supporting (aligned to +/- strand): ref base A (6/10); new base G (3/6); total (9/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACT > NC_000913/3269940‑3270225 | acagCCCCACCAAAATAAAAAACCCGCCGAACCGGGTTATTACGTAACACAGGTCAAACTGACCGATCACCCGCTTTCATTTGGGTAAAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCAGCGGCggg < 1:845050/146‑1 (MQ=11) tcccacTCAAAAATTATAAACCCGCCGTATCGGGTTTTTACGTAAAACAGGTCAAACTGACCGATAAGCCGCTATCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt < 1:587973/144‑1 (MQ=11) ccccTCCAAAAGTATAAACCCGCCGTAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt < 1:1793879/149‑1 (MQ=11) ccacTCCACAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttt < 1:222281/146‑1 (MQ=11) cacTCAAAAATTTAAAACCCGCCGTAGCGGGTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt < 2:1294893/147‑1 (MQ=11) cacTCAAAAATTATAAACCCGCCGTATCGGTTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt < 2:936917/147‑1 (MQ=11) tcaaaGCATAAACCCGCCGCATCGGGTTATTACGTTAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgcttt < 2:140686/147‑6 (MQ=11) aaaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGGATAGTGTCGTGGCCAGTCATTCATGATAGTTCACCGCCAACACCAAAAACCCGGCGCAGCGCGTTGTTAAGtga > 1:676128/1‑147 (MQ=11) aagcttaaaCCCGCCTTATCGGGTTTTTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc < 1:363557/143‑1 (MQ=11) tAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTATTTTGGGTATAGTGTAGTTGAAAGTCATTCATATTTCTGCCCCAACAAAAGCAAAAAACCGCCGAAGCGGGTTTTTTCGTTAACCAg > 2:1458654/1‑149 (MQ=11) tAAAAACCCGCTTCGGCGGGTTTTTACGTAAAACAGGTGAAACTGAACGATAAGCCGCTTACTTTTGGGTATAGAGTAGTGGACAATGATTGATCTTTATTCAACTAAAAAATCAAAAACCCGCCAAAAAGAGATTTTAAGTAAATCAg > 2:412873/1‑149 (MQ=255) cgccgTACCGGTTTTTTACGTACAACAGGTTAACCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt < 2:1868284/149‑1 (MQ=11) aaCAGGTGAAATTTATAGATAAGCGGCTTTCTTTTTGTTATAGTGTCGTGTACAGTCATTCCTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCTATGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg < 2:958485/149‑1 (MQ=11) ccagtcgAAACTGCCCGATATCCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGTTGAAAGTGACCGATAAGCCGCTTTCTTTTgg < 1:1387663/143‑1 (MQ=11) aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg < 1:1217227/108‑1 (MQ=35) gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg < 1:1075865/108‑1 (MQ=35) tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa < 2:1782609/124‑1 (MQ=17) attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGa < 2:413188/75‑1 (MQ=34) gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt < 1:320727/64‑1 (MQ=35) cAAAAACCCGCCGACGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg < 1:1458654/149‑1 (MQ=38) aaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTAGAGGCCAGTCAATCATATTTCGGCACCAACAAAAGCAAAAACCCGGCGAGGTGGGTTGTTAAGTTTATCAgg > 1:407202/1‑149 (MQ=2) aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTAGAAAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTTAATCAgg > 1:150017/1‑149 (MQ=255) aaaaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGAATAGGCAAGTCAATCAAAATTATGCCCCAACAAAAACAAAAACCCGCAGCAGAGGGTTGTTACCGAAATCaag > 2:584933/1‑147 (MQ=2) aaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTGCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTTAATGAggg > 1:12429/1‑148 (MQ=11) aaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTGCAAAAGCAAAAACCCGCGGAAGCGGGTTTTTTCGTTAATCAggg > 1:4995/1‑148 (MQ=11) aCCCGCCGCACCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa < 1:1768500/149‑1 (MQ=255) cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCCTCGTCGAAAGTCATTCATAATTCTGCCCCGCCTAAAGCCAAAAACCGCGGAAGGGGGTGTTTACGTAAATCAGGAGAAAca > 1:322376/1‑148 (MQ=11) | TCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACT > NC_000913/3269940‑3270225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |