Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,182,809	C→A	S522Y (TCT→TAT)	rpoB →	RNA polymerase, beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,182,809	0	C	A	100.0%	284.4 / NA	91	S522Y (TCT→TAT)	rpoB	RNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (41/50);  total (41/50)

CGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGT  >  NC_000913/4182665‑4182954                                                                                                                                                 |                                                                                                                                                  cGGTGAAAGAGCCTCTGTCTCTGGGCGATCTTGATACCCTGATGCCACATGATATGATCAAAGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCTGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATgag                                                                                                                                               <  1:1280793/149‑1 (MQ=255)  ggTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATgaga                                                                                                                                              <  1:1533667/149‑1 (MQ=255)         gagCGGCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGca                                                                                                                                       <  2:1312256/149‑1 (MQ=255)           gCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGCTCAACGCCAAGCCGATTTCCGCCGCAGTGAAAGAGTTCTTCGGTTCCCGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGcaca                                                                                                                                     >  2:1302105/1‑149 (MQ=255)                tgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgt                                                                                                                                <  2:1047577/149‑1 (MQ=255)                tgtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgt                                                                                                                                <  2:806707/149‑1 (MQ=255)                 gtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgtc                                                                                                                               <  2:770423/149‑1 (MQ=255)                 gtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgtc                                                                                                                               <  2:1465531/149‑1 (MQ=255)                 gtctCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgtc                                                                                                                               <  2:1776039/149‑1 (MQ=255)                 gtctCTGGGAGATCTGGATACCCTGAGGCCACAGGATATGATCATCGCCAAGCCGATTTCCGCATCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAAcgtc                                                                                                                               <  1:1516461/149‑1 (MQ=255)                  tctcTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCCAGCCGATTTCCGCAGCACTGAAAGAGTTCTTCGGATCCAGCCTGCTGACTCAGTTTATGGACCAGAACAACCCGCTGAATGAGATTACGCTCAAAcgtcg                                                                                                                              >  1:1718075/1‑149 (MQ=255)                       gggCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTAtct                                                                                                                         <  2:708763/149‑1 (MQ=255)                       gcgCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGCAAGAGTTCTTCGGTTCCAGCCAGCTGGCTCAGTTTATGGACCAGCTCCACCCGCTGTATGAGATTCCGCCCACACGTCGTAtct                                                                                                                         >  2:1667246/3‑149 (MQ=255)                        ggCGATCTGTATACCCTGATGCCACATGATATGATCACCGCCAAGCAGATTTCCGAAGCAGTGAAAGAGTTCTTCTGTTCCAGCCAGCTTTCTCAGTTTATGGACCAGAACAACCCGCTGTATCAGATTACGCACAAACGTCGTAtctc                                                                                                                        <  1:1590857/149‑1 (MQ=255)                             tCTGGATACAATGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCATTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCAc                                                                                                                   >  2:108971/1‑149 (MQ=255)                                ggATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACtag                                                                                                                >  2:594844/1‑147 (MQ=255)                                ggATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCg                                                                                                                >  1:890838/1‑149 (MQ=255)                                 gATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCgg                                                                                                               <  1:359859/149‑1 (MQ=255)                                  aTACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCATTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGc                                                                                                              <  2:1428737/149‑1 (MQ=255)                                     ccatGATGCCACAGGATATGATCAACGCCAAGCCTATTTCCTCATCAGTTAAAGAGTTCTTAGTTTCCAGCCAGCTGTATCATTTTATGGAACAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCa                                                                                                           <  2:579370/146‑1 (MQ=255)                                      ccTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAg                                                                                                          >  2:506791/1‑149 (MQ=255)                                       cTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAgg                                                                                                         >  1:913022/1‑149 (MQ=255)                                       cTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACACGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAgg                                                                                                         >  1:1673380/1‑149 (MQ=255)                                         gATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCg                                                                                                       >  2:841208/1‑149 (MQ=255)                                              cacaGGTTTTGATCAACGCGAAGCCGATTTCCGCAGCAGTTAAAGAGTTCTTCGGTTCCAGCCAGCAGTCTCAGTTTATGGACCATAACAACCCGCGGTATGCGTTTAGGCACACACGTCGGATCACCGCACTCGCCGCAGTCGGTCTg                                                                                                  >  2:440858/1‑149 (MQ=255)                                               acaGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGa                                                                                                 >  1:1447161/1‑149 (MQ=255)                                                caGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc                                                                                                <  2:696740/149‑1 (MQ=255)                                                caGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTGCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAc                                                                                                <  2:1530389/149‑1 (MQ=255)                                                  ggATATGATCACCGCCAAGCCGATTTCCGCAGCAGTGAAAGAGGTCTTCGGTTCCAGCCTGCTGTCGCAGTGTATGGAGCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGAccc                                                                                              <  1:1523184/149‑1 (MQ=255)                                                     tatGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTg                                                                                           >  2:272822/1‑149 (MQ=255)                                                     tatGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTg                                                                                           <  1:272822/149‑1 (MQ=255)                                                     tatGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTg                                                                                           <  1:69295/149‑1 (MQ=255)                                                        gATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAAc                                                                                        >  2:658503/1‑149 (MQ=255)                                                          tCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGt                                                                                      >  1:1278591/1‑149 (MQ=255)                                                          tCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGt                                                                                      >  1:1532933/1‑149 (MQ=255)                                                          tCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAAAAACACGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGt                                                                                      >  1:449601/1‑149 (MQ=255)                                                           cAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTg                                                                                     <  2:1831517/149‑1 (MQ=255)                                                           cAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTg                                                                                     <  2:456523/149‑1 (MQ=255)                                                              cGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACTACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAg                                                                                  >  1:578669/1‑149 (MQ=255)                                                                ccAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAATCGTCGTATATCCGCACTCGGCCCAGGCGGTCTGACCCGGGAACGAGCAGGc                                                                                >  2:1717749/1‑149 (MQ=255)                                                                  aaGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCtt                                                                              >  2:1512158/1‑149 (MQ=255)                                                                     ccGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGTACGTGCAGGCTTCGa                                                                           >  2:1530149/1‑149 (MQ=255)                                                                     ccGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGa                                                                           >  1:700983/1‑149 (MQ=255)                                                                        aTTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCTAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGt                                                                        >  1:290615/1‑149 (MQ=255)                                                                          ttCCGGAGCAGTGAAAGAGTGCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCATAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc                                                                      <  2:35933/149‑1 (MQ=255)                                                                          ttCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTc                                                                      >  2:1321038/1‑149 (MQ=255)                                                                           tCCGCAGCAGTGAAAGAGTGCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCg                                                                     <  2:890838/149‑1 (MQ=255)                                                                            ccGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCga                                                                    <  1:1704913/149‑1 (MQ=255)                                                                            ccGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCga                                                                    <  1:436191/149‑1 (MQ=255)                                                                             cGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCgag                                                                   <  1:1620706/149‑1 (MQ=255)                                                                                 gcagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGt                                                               <  1:611092/149‑1 (MQ=255)                                                                                  cagTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTa                                                              <  2:616264/149‑1 (MQ=255)                                                                                       aaaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGTCTTCGAAGTTCGCGACGTACcccc                                                         >  2:348205/1‑149 (MQ=255)                                                                                       aaaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACAccc                                                         >  1:452429/1‑149 (MQ=255)                                                                                        aaGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCGTCGAAGTTCGAGACGTACACCCg                                                        >  1:781874/1‑149 (MQ=255)                                                                                         agagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGa                                                       <  2:489060/149‑1 (MQ=255)                                                                                         agagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGGCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGa                                                       <  1:797518/149‑1 (MQ=255)                                                                                          gagTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGAc                                                      >  2:554916/1‑149 (MQ=255)                                                                                           agTGCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACt                                                     <  1:1313163/149‑1 (MQ=255)                                                                                             ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCa                                                   <  2:1447161/149‑1 (MQ=255)                                                                                             ttcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCa                                                   >  1:789870/1‑149 (MQ=255)                                                                                              tcttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCAc                                                  <  1:108971/149‑1 (MQ=255)                                                                                                ttcGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTa                                                >  2:925814/1‑149 (MQ=255)                                                                                                  cGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGGACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGTGACGTACACCCGACTCACTACg                                              >  2:409695/1‑149 (MQ=255)                                                                                                    gTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGTCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGt                                            >  2:1583404/1‑149 (MQ=255)                                                                                                     ttCCAGCCAGCTGTCTCAGTTTATGGACCAGAACATCCCGCTTTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTGCGAAGTTCGAGACGTACACCCGACTCACTACGGTc                                           <  1:1279431/149‑1 (MQ=255)                                                                                                      tCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTATGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTcg                                          >  1:142316/1‑149 (MQ=255)                                                                                                       ccagccagCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTcgc                                         <  2:1320678/149‑1 (MQ=255)                                                                                                       ccagccagCTGGCTCAGTTTATGGACCAGACCAACCCGCTGTATGAGATTACGCACAAACGGCGTATCTCCGCACTCGGCCCCGGCGGTCTGACCCGTGAACGGGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGgcgc                                         <  2:479581/149‑1 (MQ=255)                                                                                                         agccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGt                                       <  1:302892/149‑1 (MQ=255)                                                                                                           ccagcTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTAt                                     <  2:913022/149‑1 (MQ=255)                                                                                                           ccagcTCTCTCAGTTAATGGAACACAACAACCCGCAGTATAAGATAACGCACAAACGTCGTATCAACTCACTCCGCCCAGGCGGTCTGACCCGTGAACGTGCACGCTTCGAAGTTAGAGACCTACACCCGACTCACGACGGAAGCGTAt                                     >  1:901524/1‑149 (MQ=255)                                                                                                               cTGTCTCAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGCCCCGTGAACGTGCAGGGTTCGAAGTTCGAGACGTACCCCCGACTCACGACGGGCGCGTATGTcc                                 >  1:963990/1‑149 (MQ=255)                                                                                                                    tcAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg                            <  1:606275/149‑1 (MQ=255)                                                                                                                    tcAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCg                            >  2:1116645/1‑149 (MQ=255)                                                                                                                     cAGTTTATGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGa                           <  1:658503/149‑1 (MQ=255)                                                                                                                     cAGTTTATGGACCAGAACAACCCGCTGGATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGCCTCACTACGGGCGCGTATGGCCAATCGa                           >  1:461472/1‑149 (MQ=255)                                                                                                                            tGGACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGGGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTAACTACTGTCGCGTATGTCCAATCGAAACACCt                    >  1:859637/1‑149 (MQ=255)                                                                                                                              gACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                  >  2:52208/1‑149 (MQ=255)                                                                                                                              gACCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGGTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                  <  1:1302105/149‑1 (MQ=255)                                                                                                                              gACCAGAACAACCAGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGa                  <  2:1668749/149‑1 (MQ=255)                                                                                                                               aCCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGGGGGCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCTCTACGGTCGCGTATGTCCAATCGAAACCCCTGaa                 <  2:1829815/149‑1 (MQ=255)                                                                                                                               aCCAGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGaa                 >  2:1409623/1‑149 (MQ=255)                                                                                                                                ccAGAACCACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGGCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAg                <  1:1756510/149‑1 (MQ=255)                                                                                                                                  aGAACAACCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGt              <  2:789870/149‑1 (MQ=255)                                                                                                                                     acaacCCGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCg           <  1:795532/149‑1 (MQ=255)                                                                                                                                       aacCCGCTGTAGGAGATTACGCAAAAACGTCGTATCTCCGAACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa         <  1:841208/149‑1 (MQ=255)                                                                                                                                          ccGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt      <  1:1512158/149‑1 (MQ=255)                                                                                                                                           cGCTGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATc     >  2:815804/1‑149 (MQ=255)                                                                                                                                              tGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt  <  1:554916/149‑1 (MQ=255)                                                                                                                                              tGTATGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGt  <  2:452429/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CGGTGAAAGAGCGTCTGTCTCTGGGCGATCTGGATACCCTGATGCCACAGGATATGATCAACGCCAAGCCGATTTCCGCAGCAGTGAAAGAGTTCTTCGGTTCCAGCCAGCTGTCTCAGTTTATGGACCAGAACAACCCGCTGTCTGAGATTACGCACAAACGTCGTATCTCCGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGT  >  NC_000913/4182665‑4182954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑