Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1978503–1979269	1979278	10–776	50 [0]	[0] 50	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1978502	0 (0.000)	48 (0.970)	43/266	0.2	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913/1979133‑1979421                                                                                                                                                   |                                                                                                                                               aaatgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgtat                                                                                                                                              <  1:125211‑M2/3‑1 (MQ=255)   atgggtctttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATaa                                                                                                                                            <  1:875938‑M2/5‑1 (MQ=255)          tttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacataccacggaggggggctgcgattttcaataatccgtgaggcagatcacacaaaacactcaattacttaccataaatgTACAAGTCATAc                                                                                                                                     >  2:800689‑M2/138‑149 (MQ=255)           ttacacttatctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcaaatcacacaaaacactcaattacttaccataaatgTTTAAGTCATACt                                                                                                                                    >  2:31674‑M2/137‑149 (MQ=255)                     ctaagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcattaatgcgtgattcagagcacacaaaacgctcaattacttaacataaatgTATAAGTCATACTTTTGTTTTgg                                                                                                                          <  1:183526‑M2/23‑1 (MQ=255)                      taagagttttcctaactcgacggaactgtattcgtcagtacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTggg                                                                                                                         <  1:68615‑M2/24‑1 (MQ=255)                       aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                                                                        >  1:395942‑M2/125‑149 (MQ=255)                       aagatttttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataaggcgtgatgcagatcacaaaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGgt                                                                                                                        >  1:846791‑M2/125‑149 (MQ=255)                            ttttcctaaatcgacgcaaccgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgcgatgcagatcacacaaaacactcaattacgtaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAtt                                                                                                                   <  2:353627‑M2/30‑1 (MQ=255)                             tttcctaaagcgacgcaactggactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTAttt                                                                                                                  <  2:178900‑M2/31‑1 (MQ=255)                              ttcctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTc                                                                                                                 >  1:256487‑M2/118‑149 (MQ=255)                                 ctaaatcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt                                                                                                              <  1:483989‑M2/35‑1 (MQ=255)                                     atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATATTTTTGTTTTGGGTGTATTTCAAtctct                                                                                                          >  1:756170‑M2/111‑147 (MQ=255)                                     atcgacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt                                                                                                          >  2:525056‑M2/111‑149 (MQ=255)                                        gacgcaactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaa                                                                                                       >  2:183402‑M2/108‑149 (MQ=255)                                           ggaactgtactcgttaccacacgtaaatacaacggaggggggctcacattttcaataatgcgtgatgcagatcacacaaatctctcaagtacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaaaa                                                                                                    <  1:356077‑M2/45‑1 (MQ=255)                                             aactgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGTTGTATTTCATTCTGTTAAAAAGt                                                                                                  >  2:224378‑M2/103‑149 (MQ=255)                                              actgtactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGtt                                                                                                 <  1:525056‑M2/48‑1 (MQ=255)                                                  tactcgtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATAGTTTTTTTTTGGGTGTATTTCAATCTGTTAAAAAgtttttt                                                                                             >  1:248877‑M2/98‑148 (MQ=255)                                                       gtcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctac                                                                                        >  1:846842‑M2/93‑149 (MQ=255)                                                        tcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCCTACTTTAGTTTTGGGTGTATTTCACTCTGTTAAACAGTTTTTcgctacg                                                                                       >  1:857601‑M2/92‑149 (MQ=255)                                                        tcactacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacg                                                                                       >  1:816942‑M2/92‑149 (MQ=255)                                                         cactacccgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgc                                                                                      <  2:757530‑M2/59‑1 (MQ=255)                                                           ctacacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTcgctacgcta                                                                                    <  2:627711‑M2/61‑1 (MQ=255)                                                              cacgcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagca                                                                                 <  1:409925‑M2/64‑1 (MQ=255)                                                                 gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaacccctgccgtacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTACAAAGTTTTTCGCTACGCTagcaagc                                                                              >  1:901960‑M2/83‑149 (MQ=255)                                                                 gcacatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaagc                                                                              >  1:415718‑M2/83‑149 (MQ=255)                                                                   acatacaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAgcaagcaa                                                                            >  1:469481‑M2/81‑149 (MQ=255)                                                                       acaacggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAAt                                                                        >  2:136231‑M2/77‑149 (MQ=255)                                                                         cgagggggggggcggcgagtttcaatgatgagtgatgcagatcacacaaagcgctcaattacttaacataaatgTATAAGTCAGACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGa                                                                      <  2:340029‑M2/75‑1 (MQ=255)                                                                           cggaggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaaa                                                                    <  1:224378‑M2/77‑1 (MQ=255)                                                                              aggggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAg                                                                 <  2:388115‑M2/80‑1 (MQ=255)                                                                                gggggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAGGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGa                                                               >  2:848460‑M2/68‑149 (MQ=255)                                                                                  gggctgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGaat                                                             >  1:331123‑M2/66‑149 (MQ=255)                                                                                      tgcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATc                                                         >  1:738790‑M2/62‑149 (MQ=255)                                                                                       gcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaa                                                                     <  2:92380‑M2/76‑1 (MQ=255)                                                                                       gcgattttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGaa                                                                     >  1:92380‑M2/61‑136 (MQ=255)                                                                                            tttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                >  2:740642‑M2/56‑120 (MQ=255)                                                                                            tttcaataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTagcaa                                                                                <  1:740642‑M2/65‑1 (MQ=255)                                                                                                aataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAATCATACTCTTGTTTTGGGTGTATTTCAATATTTTAAAAACTTTTTCTCTACGCTAGCAAGCAAAAATGAACAAGGAATAATCGAAATAGGAt                                               >  2:508392‑M2/52‑149 (MQ=255)                                                                                                 ataatgcgtgatgcagatcacacaaaacacttaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATg                                              <  2:816942‑M2/99‑1 (MQ=255)                                                                                                 ataatgcgtgatgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGTTACGCTTGCAAGAAAAAATAAAAAAGGAATAAacgacatatgctg                                              >  1:65842‑M2/51‑143 (MQ=255)                                                                                                            tgcagatcacacaaaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTAAAAAAAAAAAAAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGt                                   <  2:395942‑M2/110‑1 (MQ=255)                                                                                                                         aaacactcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCAc                      <  2:368281‑M2/123‑1 (MQ=255)                                                                                                                             actcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAATTGGGATGTTGCGCACAGTCACAATAACTCACCGTa                  >  1:304813‑M2/23‑149 (MQ=255)                                                                                                                               tcaattacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTCTATGCAGGTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTaaa                <  1:634801‑M2/129‑1 (MQ=255)                                                                                                                                  attacttaacataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAaataa             <  2:256487‑M2/132‑1 (MQ=255)                                                                                                                                      cttaacataaatgTATTAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGATTTACGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAAAaatcac         <  2:846791‑M2/136‑2 (MQ=255)                                                                                                                                            ataaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCta   >  2:60‑M2/8‑149 (MQ=255)                                                                                                                                             taaatgTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCtat  <  1:89425‑M2/143‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTAT  >  NC_000913/1979133‑1979421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑