| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 3,560,455 | 1 | . | G | 95.8% | 148.0 / ‑1.3 | 49 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base G (19/27); total (20/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTT > NC_000913_3_pae_tpiA/3560308‑3560592 | ccGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGA‑T‑GGcg > 1:263221/1‑148 (MQ=255) cGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGCCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATc < 1:226559/149‑1 (MQ=255) tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCCAGCCTCTT‑TCTGAATTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAgg > 1:799441/1‑149 (MQ=255) aaCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTCTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGtt < 1:733130/149‑1 (MQ=255) aGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTg < 1:265699/149‑1 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCACCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTcc < 1:531683/149‑1 (MQ=255) aGCCATTGGGGATTTGCTCTGCCACTTTGAGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGTACTGGAAGGGAGCGCCGCACCGCCGTGA‑T‑GGCGCCGGGTCAGGTTTTGCTCGGcc > 2:609989/1‑149 (MQ=255) aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGcc > 1:512792/1‑149 (MQ=255) gCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTCTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCa > 1:492161/1‑149 (MQ=255) ccATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGCAAGGTAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAg < 1:379772/149‑1 (MQ=255) cATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGc > 1:490432/1‑149 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGTCAGCTCAtt < 2:528147/149‑1 (MQ=255) gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt < 1:91015/149‑1 (MQ=255) gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa < 1:802159/149‑1 (MQ=255) gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcg > 1:851406/1‑149 (MQ=255) cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGTCTGGGGATCGTGCG‑ACGGCGTGTTAGCCGAACTGGTTTGCAGCGCCGCACCGCCTTGA‑T‑GGCTCAGGGTCAGGTTTTCCTCCTGCATCTCATAGAGGTctc > 2:349162/1‑147 (MQ=255) tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgcg > 1:762791/1‑149 (MQ=255) cTTGGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa < 1:7582/149‑1 (MQ=255) gCGATGCGCTCTGTTTCTTCGGGCTGGGTGGCTTTGTGATCGTGCC‑GTGGCTTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCTAGCTCATTGAGGTCGCGGCGAATAGTCTGCgg < 2:404949/149‑1 (MQ=255) ctTTTTCTTGGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgag < 1:362317/149‑1 (MQ=255) ttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa < 1:741979/149‑1 (MQ=255) ttcttcGGGGCGGTTGTCCTTGCGGTTGTGGC‑GCGGCTTGTTAACCGTACTCGATGGCAGTGCCGACCCCCGATGA‑T‑TGCGCAGGATCAGGTTTTGACCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 2:214606/149‑1 (MQ=255) ctcttcGGTCTGGGTGGCGTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAAt < 2:113538/148‑1 (MQ=255) ttcGGTCTGGGTGGCCTTGCGCTCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAACGct > 2:363979/1‑149 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGctct < 1:133123/149‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct < 1:459575/144‑1 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGct > 2:459575/1‑144 (MQ=255) tCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAcc > 2:466878/1‑149 (MQ=255) ggggTGGCCTGGCGATCGTGCC‑ACGGCGTGCTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg < 1:449374/148‑1 (MQ=255) ccTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTTGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg < 1:143095/149‑1 (MQ=255) cTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:648206/1‑149 (MQ=255) cTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:484269/1‑149 (MQ=255) cTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCgg > 1:231005/1‑149 (MQ=255) ttGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGt > 1:626056/1‑149 (MQ=255) aTCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa > 1:695018/1‑149 (MQ=255) tGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATa > 1:46440/1‑149 (MQ=255) ggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg < 2:851406/149‑1 (MQ=255) gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc > 1:258962/1‑149 (MQ=255) ggtgtTACGCGAACGGGAGGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCATGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:58967/148‑1 (MQ=255) agtgtTAACTGAACTGGGTGGCAGCGCCGCACCGCCATGA‑T‑GTCGGAGGATCAGGTTTGGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgct < 2:486226/148‑1 (MQ=255) ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt < 1:741985/149‑1 (MQ=255) aaCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGt < 2:231005/149‑1 (MQ=255) aaCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGGTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACCGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTCCCGTTTACCCAGt > 2:665368/1‑149 (MQ=255) acgggAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:391172/146‑1 (MQ=255) aCTGGAATGCAGCGACGCACCGCCATGA‑T‑GGCGCAGTATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCCGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 2:304027/149‑1 (MQ=255) aCTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt < 1:352048/149‑1 (MQ=255) cTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCATGAGAAATGTTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc < 1:378525/149‑1 (MQ=255) ggaGGCAGCGGCGCACCGTCAGGAGG‑GGG‑CGGGATCAGGTTTTGCTTGGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGat < 2:195934/147‑1 (MQ=255) aGCGCCGCACCGCCATGA‑T‑GGCGCAGGATCAGGTTTTGCTCCGCCAGCTAATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGACTTTCGGTAATGAAATCACCATCCTGTTAGACCAGTTCGATAATAcc > 1:113013/1‑149 (MQ=255) gCCATGA‑TGGGC‑GAGGAGGAGGGTTTGCTCCGCCAGCTCGTTGAGTGCGCGGCGAATGGTCTGCGGGCTGACGGACAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTGTCTAGTTCGATAATACCGTTGTGACGtt < 2:692665/149‑1 (MQ=255) | CCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCC‑ACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGA‑T‑GGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTT > NC_000913_3_pae_tpiA/3560308‑3560592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |