| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_tpiA | 4,296,297 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 1 | . | C | 96.9% | 79.9 / ‑3.7 | 32 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (14/17); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_pae_tpiA | 4,296,296 | 2 | . | G | 96.9% | 78.2 / ‑3.7 | 32 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (14/17); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCT > NC_000913_3_pae_tpiA/4296213‑4296434 || aCGCTTAACGCGTCTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGt > 1:763793/1‑149 (MQ=255) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATTAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGt > 1:160131/1‑149 (MQ=255) ggcgTCTATTCAGGTCAACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATCCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt < 1:561111/148‑1 (MQ=11) cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTc < 2:114888/149‑1 (MQ=255) ttATCAAGCCTACGCCAGACAGCGCAATGGCCTGATTTATCGTGATTTTGTAGCTCGGATAAGGCGTGTACGCCG‑‑CATCCGAAATCAATGCCTGATGCGACGCTTGCCCCGTCTTATCAGGCCTATCTTAAACGTCGTTTACTTTTTCa > 2:208457/1‑149 (MQ=11) aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa < 1:267184/149‑1 (MQ=255) tcaCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca < 1:264381/147‑1 (MQ=255) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCttta > 2:789837/1‑147 (MQ=255) gcAATAGCCTGATTTAGCGTGATGTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:444408/149‑1 (MQ=255) aGCCTGATTTAGCGTGATTTTTTAGGTCGGATCAGGCGTTTACGCCG‑‑CATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCtt < 2:581245/149‑1 (MQ=18) cagtgtttAGCGTTATTTGGTAGGGCGGATAGGGGGTTTACGCCG‑‑CATCCGGCATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTcc < 2:113451/144‑1 (MQ=21) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa < 2:160131/149‑1 (MQ=255) gATTTAGCGAGATTTTGTAGCTCGCATACGCCGTTTA‑‑CCGCTCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 2:465616/149‑1 (MQ=25) aTTTTGCTTGATTTTGGAGGCCGGATAAGGTGTTTACGCCG‑‑CATCCGACATCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc < 2:642303/149‑1 (MQ=21) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGTCTATCGTAACCTTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACa < 2:393376/149‑1 (MQ=21) ttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAg < 1:445197/149‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 1:181065/149‑1 (MQ=255) cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa > 1:782001/1‑149 (MQ=255) gTGATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATACAACTCAg > 2:654466/1‑149 (MQ=21) gATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt > 1:847939/1‑149 (MQ=255) gATTTTGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt < 2:250066/149‑1 (MQ=255) gATTTGGTGGGTGGGATACGGCGTTTACGCCT‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt < 2:165208/149‑1 (MQ=25) ttttGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCCAACCCTTCCATACAGCTCAGGTTc > 2:151818/1‑149 (MQ=25) ttttGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTaa > 2:244527/1‑86 (MQ=1) ttttGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTaa < 1:244527/86‑1 (MQ=1) ttttGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc > 2:316587/1‑149 (MQ=255) ttGTAGGTCGGATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa < 1:87195/149‑1 (MQ=255) ggATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGGCTGATGGGACGCTGGCCGCGTCTTGTCAGGCCTGTCTTAGCCGGTGGTTAATTTTTCAAACTCTTCACACCCGGTAGAACAACCGTCCAAACCGCGCAGGTTAAACCAGtcacg > 2:73969/1‑145 (MQ=12) gATAAGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTCACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACACCTCAGGTTCAACCAGtatagc > 1:455434/1‑144 (MQ=25) aGGCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACAGTTGGTTAATTTTTCAAACTCTTCAACACCGGTATCACACCCTTACATACAGCTCAGGTTCAACCAGTACacagagt > 2:511213/1‑143 (MQ=21) ggCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg > 2:205925/1‑75 (MQ=14) ggCGTTTACGCCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTg < 1:205925/75‑1 (MQ=14) || ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCG‑‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCT > NC_000913_3_pae_tpiA/4296213‑4296434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |