Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,604,632 C→A A186E (GCA→GAA)  bglJ → bgl operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,604,6320CA96.2% 160.0 / NA 54A186E (GCA→GAA) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (23/28);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604489‑4604773
                                                                                                                                               |                                                                                                                                             
cgaccgacTGAACAATCAGTGGTACATTAACCAAACCCGGACGTTAAGCCGGACGGAGAGAGAAATATTGCGCTTTATGTAGCGTGCCTACTTAGTTACACAAATTGCCGAGCAGCTTAGACGCAATATCCAAACGCTCCGCGCACATa                                                                                                                                          >  2:334371/1‑149 (MQ=255)
   cggacTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAAt                                                                                                                                       <  2:458359/147‑1 (MQ=255)
        tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  >  1:145574/1‑149 (MQ=255)
        tGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAAt                                                                                                                                  >  2:126372/1‑149 (MQ=255)
             aaTCAGTGTTACATGAACGAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAt                                                                                                                             <  2:377314/149‑1 (MQ=255)
             aaTCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGAt                                                                                                                             >  1:720991/1‑149 (MQ=255)
               tCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGt                                                                                                                           >  1:316357/1‑149 (MQ=255)
                   tGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGGACATAAATTTAATGTGATGTCGaa                                                                                                                       >  1:815907/1‑149 (MQ=255)
                            aaCCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGt                                                                                                              <  1:126372/149‑1 (MQ=255)
                             aCCACAGCCGGACGTTAAGCCCGACAGAGAGAGAAATCTTGCGCTTTATGTCGCGTGGCTACTCAACGACACAAATTGCCGAGCAGCTTAAACGCAGTATCAAAACGATCCCTGAACATAACATTCTTGGCGTGTCGAAACTggtgtgt                                                                                                             >  2:387399/1‑144 (MQ=255)
                              ccAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGACTACTCAATGACACAAATTGCCGAGCCGCTTACACCCAACACTAAAACGAACCGTGAACAAAAATTTAATGTGATGTCCAAAATGGGCGTCa                                                                                                            >  2:261394/1‑149 (MQ=255)
                               caaattcGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACAAAAATTTAATGTGATGTCGAAACTGGGCGTCAg                                                                                                           >  2:651821/7‑149 (MQ=255)
                               cAAAGCCGGACGTTAAGACCGACGGAGAGAGAAATATTGCGCGTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAAACAAATATTAAAACAATCCTGGAACATAAATTTAATGTGATGTCGAAACTGGGCATCAg                                                                                                           >  2:426986/1‑149 (MQ=255)
                                    ccGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAGCATAAAGTTAATGTGATGTCGAAACTGGGCGTCAGTTCTg                                                                                                      >  1:795302/1‑149 (MQ=255)
                                     cgtgccgtAAGCCAGACGGATAGAGAAATAGTGCGCTTTATGGCGTGTGGCTACTCAAGGACACAAATTGCCTAGCAGCTTAAACGCAATATCAAAACGATCTGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGa                                                                                                     <  2:379409/142‑1 (MQ=255)
                                         cGTTAGGCCCGGCGGAGAGAGTAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCa                                                                                                 <  1:75919/149‑1 (MQ=255)
                                         cGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCGATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCCAAACTGGGCGTCAGTTCTGACGCa                                                                                                 >  2:655193/1‑149 (MQ=255)
                                          gTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAg                                                                                                <  2:832630/149‑1 (MQ=255)
                                             aaGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACGCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGgt                                                                                             >  2:290860/1‑149 (MQ=255)
                                                  cGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGttgttg                                                                                        <  2:498459/149‑1 (MQ=255)
                                                    aCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                      <  1:736246/149‑1 (MQ=255)
                                                    aCGGAGAGAGAAACATTGCGGTTTTGGTCGCGTGGCTACTCAATGACAAAAAGTGCCGAGCAGCTTAAACGCAATATCAAAACGAGCCGGGAACATAAATTTAATGTGATGTCAAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                      <  2:336925/149‑1 (MQ=255)
                                                     cGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAg                                                                                     <  1:286390/149‑1 (MQ=255)
                                                              aaaTATTGCGCTTTATGTCGCGTGGCTACTCAATGAAACAAAGTACCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGCTGTCGAAACTGGGCGTCGGTTCTGACGCAGGGTTGTTGGAGGCCGCAGat                                                                            >  1:202566/1‑149 (MQ=255)
                                                                 tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATAtt                                                                         >  1:593251/1‑149 (MQ=255)
                                                                 tatTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATAtt                                                                         >  1:826517/1‑149 (MQ=255)
                                                                    tGCGGTTTATGTCGCGTGGCTCAGCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTg                                                                      <  2:153968/149‑1 (MQ=255)
                                                                    tGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                      <  1:60616/133‑1 (MQ=255)
                                                                    tGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGa                                                                                      >  2:60616/1‑133 (MQ=255)
                                                                     gcgcTTCATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGt                                                                     <  1:52200/149‑1 (MQ=255)
                                                                       gcTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTta                                                                   <  1:394633/149‑1 (MQ=255)
                                                                        cTTTATGTCGCGTGGCTCCTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTtat                                                                  <  1:606421/149‑1 (MQ=255)
                                                                                         aCTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGaa                                                 >  1:823525/1‑149 (MQ=255)
                                                                                             aacgccacaAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTCGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa                                             <  1:104626/144‑1 (MQ=255)
                                                                                             aaTGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACaa                                             >  2:632455/1‑149 (MQ=255)
                                                                                                 acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTg                                                     >  2:570577/1‑137 (MQ=255)
                                                                                                 acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTg                                                     <  1:570577/137‑1 (MQ=255)
                                                                                                 acacAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTaa                                         <  1:553623/149‑1 (MQ=255)
                                                                                                         tGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCTGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGc                                 <  1:640215/149‑1 (MQ=255)
                                                                                                           ccGAGCAGCTTAAACGCAATATTAAATCGATCCGCGCACATAAATTTAATGGGATGTGGAAACTGGGCGTCAGTCCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCAt                               <  1:11611/149‑1 (MQ=255)
                                                                                                           ccGAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCAt                               <  2:690015/149‑1 (MQ=255)
                                                                                                             gAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCGGCAGATATTCTGTTAGGTATGCGGCATTGCGAAACAAGTAATGTGGTGCATcc                             >  1:663417/1‑149 (MQ=255)
                                                                                                             gAGCAGCTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATcc                             <  1:290860/149‑1 (MQ=255)
                                                                                                                cagcTTAAACGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTa                          <  2:559170/149‑1 (MQ=255)
                                                                                                                 agcTTAACCGCAATAGCAAAACGTTCCGTGAACATAAATTTAATGTGATGTCGAAACTTGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGACATTCGTTTATGTATGCGGCATTGCGAAACAAGTAATGGTTTGCTTCCCTAt                         <  2:102716/149‑1 (MQ=255)
                                                                                                                      aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                   >  1:781635/1‑134 (MQ=255)
                                                                                                                      aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                   >  1:781209/1‑134 (MQ=255)
                                                                                                                      aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                   <  2:781209/134‑1 (MQ=255)
                                                                                                                      aaaCGCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGtt                                   <  2:781635/134‑1 (MQ=255)
                                                                                                                          gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCa                <  1:512469/149‑1 (MQ=255)
                                                                                                                          gCAATATCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAAATGGGCGGCAGTTCTGACGCAAGGTTGTTGGACGCCGCAGAGCTTCAGCTATGTATCGCGCATAGCGAAACACGAACTTTGTAGCATGCGTATAAACCCGcg                >  2:29026/1‑148 (MQ=255)
                                                                                                                            gatatCAAAACGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCatg              >  2:438380/2‑149 (MQ=255)
                                                                                                                                      cGATCCGTGAACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGtt    <  2:826517/149‑1 (MQ=255)
                                                                                                                                        aTCCGTGAAAATAATTGTAATGTGATGTCGAAACTGGGCGTCAGTTCTGATGTTTGTTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTa  <  2:687232/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                             
CGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTCTGACGCAGGGTTGTTGGAGGCCGCAGATATTCTGTTATGTATGCGGCATTGCGAAACAAGTAATGTGTTGCATCCCTATTAATCCGCATGATGCCGGGTTTA  >  NC_000913_3_pae_tpiA/4604489‑4604773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑