Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	97.7%	124.6 / NA	44	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (29/14);  total (29/15)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

GCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_pae_tpiA/263955‑264243                                                                                                                                                |                                                                                                                                                  gCTCCCAGGCGCAGTGTAATCTCCCCCTGAGGGTACCTTGTCGGGTTGCTCATTACAGTTCTCCACAATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGAGGTCCTGACGGCGTTAGGCGGATACCGCTAgagc                                                                                                                                              >  2:822737/1‑146 (MQ=255)   tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                                            <  1:166476/149‑1 (MQ=255)    cccAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTcc                                                                                                                                           >  1:625929/1‑149 (MQ=255)       aGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCAACTATGGTGAGTTCGGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCCGGCGGCGTTCGGCCTATACCCCTAGGACTCCGGc                                                                                                                                        >  2:641767/1‑149 (MQ=255)             agggtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATc                                                                                                                                  <  2:525675/146‑1 (MQ=255)             aGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAATTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATc                                                                                                                                  >  1:314152/1‑149 (MQ=255)                    tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACg                                                                                                                           >  1:432065/1‑149 (MQ=255)                            tGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGcc                                                                                                                   >  1:789771/1‑149 (MQ=255)                               aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                                <  1:495774/149‑1 (MQ=255)                               aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCATATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                                >  1:643778/1‑149 (MQ=255)                                 gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGAGTTCGGCTTATACCGCTAGGACGCCGGCGATATGCGGAACGTCCCTGCCGgtgt                                                                                                              >  1:373968/1‑149 (MQ=255)                                   cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCTTCAGGCAGGAATAGGGCCGATTGCGGTCCTGGCGGCGTTCGGCGGATAGCGCTCGGCCTCACGCCGTAGCCGGAGCGTCGCTGCCGgtgtgc                                                                                                            >  1:155940/1‑147 (MQ=255)                                     cTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATg                                                                                                          >  1:351474/1‑149 (MQ=255)                                                  tCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCACTCAGGTATAGGGCCCATTCCGGTCCTGTCGGCGTTCGGCGTATCCCGCTAGGACTCCGGCGATACCCGGAACCTCCCTGCCGGGCTACTGACACACGCTcccc                                                                                             >  2:841621/1‑148 (MQ=255)                                                    aTTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTg                                                                                           >  1:365276/1‑149 (MQ=255)                                                     ttACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGGATAGGGCCCATTGCGGTCATGGCGGCGTTCGGCGTATACCGCTGGGCATCCGGGAATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCGACCGTGc                                                                                          >  1:114577/1‑149 (MQ=25)                                                         aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACa                                                                                                       >  1:204962/1‑132 (MQ=255)                                                         aGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACa                                                                                                       <  2:204962/132‑1 (MQ=255)                                                          gTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTg                                                                                     <  2:351474/149‑1 (MQ=255)                                                           ttCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGAAAGACGCTAACGTGCCACCgg                                                                                    >  2:690180/1‑149 (MQ=255)                                                            tctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGt                                                                                   <  1:463538/149‑1 (MQ=255)                                                              tcCACTATTGTCAGTTCCGGCTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGCGCTGGCGGCGTCCGGCGTATACCGCTAGCACTCCGGCGATACCAGCACCGTCCATGCCGGCGCAAGGACCGGCGATCCCGCGCCACTggact                                                                                 >  2:170894/1‑146 (MQ=255)                                                                    aTTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACGCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                           <  2:666901/149‑1 (MQ=255)                                                                    aTTGTCAGTTCAGGGGGATGGTCATCAGGCAGGTATAGGGTCCATTGCGGTCCTGGCGGTGTTCGGCGTATACCGCTAGGACTCCGGCGAGATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCg                                                                           <  2:784850/149‑1 (MQ=21)                                                                            ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTGCGGCGTATACCGCTAGGAGTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAGGCCAGACCCTCCCCCGACACTGGTCTTTGCCGGtctccta                                                                   >  2:560292/1‑143 (MQ=255)                                                                              cAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGc                                                                 <  1:826224/149‑1 (MQ=255)                                                                                 ggggATGCTATTCAGCGAGGTATAGGGGCCAGTGCGGACTGTGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATACGCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTGGCCGGTGCAGTAGCGaa                                                              <  2:70479/146‑1 (MQ=17)                                                                                 ggTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGGGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGaa                                                              >  1:240162/1‑149 (MQ=255)                                                                                           atcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                                    >  1:45998/1‑149 (MQ=255)                                                                                              aggcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTc                                                 <  1:825935/149‑1 (MQ=255)                                                                                                  aggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGGCTCAGGa                                             >  1:816790/1‑149 (MQ=255)                                                                                                   ggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGAt                                            <  1:832490/149‑1 (MQ=255)                                                                                                           gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCgg                                    >  1:459803/1‑149 (MQ=255)                                                                                                           gCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGAAGCGAAAGATTCGGGCCTCACGATGCTGGCgg                                    >  2:545121/1‑149 (MQ=255)                                                                                                           gCCCATTGCGGTCCTGGCGCCGTTCGGCGTATACCGCTAGGACTCCGGCGATATACGGAACGTCCCTGCCGGTGTAATGACACACGCTACCGTACCACCGGTATTTGCCGCTGCAGTAGCGAAGGATTCGGGACTGAGGGTGCTGGCgg                                    >  2:191632/1‑149 (MQ=255)                                                                                                               aTTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGtata                                <  2:625929/149‑1 (MQ=255)                                                                                                                 tGCGGTCCTGGCGGCGTTCGGCGTGTACCGCGATTACTCCGGCGATCCGTCGAACGTCCCTGCCGGTGTAATGACAGTCGTTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATc                              <  1:620018/149‑1 (MQ=17)                                                                                                                      tCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCAt                         >  1:532274/1‑149 (MQ=255)                                                                                                                                gTTCGGCGTCTACCGCTAGGACTCCGACGATATCCGGAACGTCCCTGCCGGTGTAAGGAGAGACGCTGGCGTGCCACTGGTATTTGCCGGTGCAGAAGCGAAACGTTCGGGTCTCAGGAGGCGGGCGGCATATGGTCAGTGccatccga               >  2:659601/1‑143 (MQ=255)                                                                                                                                 ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt              >  1:753221/1‑149 (MQ=255)                                                                                                                                        tataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGACGGCGTAACGACAGACGATACCGGGCCACTGGTGTTTGCCGGTACGGGCGCGAAAGCTTCCGGCCTCAGGATGCTGGCGGTCTGCCCTCATCCCGCTGCGTTTGCTgcg       >  2:653387/1‑147 (MQ=255)                                                                                                                                           aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                               <  1:754491/106‑1 (MQ=255)                                                                                                                                           aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGGTAAt    >  1:429028/1‑149 (MQ=255)                                                                                                                                           aCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAACGAAAGATTCGGGACTCAg                                               >  2:754491/1‑106 (MQ=255)                                                                                                                                             cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAAttt  >  1:614142/1‑149 (MQ=255)                                                                                                                                             cgctAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTCTAAGCACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAAGAAGCTGGCGGTCTCTCGTCATTGCCCTTCGTTTACTGAACAttt  >  2:283745/1‑149 (MQ=14)                                                                                                                                                |                                                                                                                                                  GCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGATAATTT  >  NC_000913_3_pae_tpiA/263955‑264243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑