Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	275,065	G→A	T18I (ACC→ATC)	insH1 ←	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	275,065	0	G	A	93.3%	34.1 / ‑6.5	15	T18I (ACC→ATC)	insH1	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (8/6);  minor base C (0/1);  total (8/7)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT  >  NC_000913_3_pae_tpiA/275009‑275207                                                         |                                                                                                                                               agacagtctcTGTTCGGGTCTGCCGGTGGTTACTGCTGGATTCGCTGTCGGCGAAGATCAGTTGACGTCTGATGATGACCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTCGTTCGCTCCTTCCGGAGCCgc                                                    <  2:646698/143‑2 (MQ=11)   aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCCTGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTa                                                  >  1:699999/1‑149 (MQ=255)           ttttCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGAACACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTgg                                          <  1:163810/149‑1 (MQ=255)               cTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTggcggc                                      <  1:301428/149‑1 (MQ=255)                tGGTCTGACGGCGCTTACTGCTGATTTCTTTGTCGGCGACAAAAAGTTGATGACTCATGATGAACCCTGTTCTATGGGTCGATATGACAAACATGTTCTTGTATCCGGTCCTTGTTCGCCCCTTCCGTGAGCGTGACGAGCTggcgcgc                                     >  2:220702/1‑146 (MQ=2)                tGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTAGGCGCTGGCGGcc                                     >  1:273326/1‑149 (MQ=255)                  gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACACTGTTCTATGGATGCAGATGACAAACATGATCTCATATCAGGGACTTGTTGGCACCTTCCGGAGGCGTTCTGAGCTGGCGGCCCt                                   >  2:622670/1‑149 (MQ=14)                     tGACGGCGCTTACTGCTGACTTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTCGCGGCCCtttt                                <  1:534592/149‑1 (MQ=255)                              ttACTGCTGAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGa                       <  2:628553/149‑1 (MQ=255)                                  tgctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGattat                   >  2:706587/1‑149 (MQ=255)                                  tgatgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGAGGCCCTTTTTGTATGTGattat                   >  2:316320/4‑149 (MQ=255)                                    ctgAATTCACTGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGAttatta                 >  1:222470/1‑149 (MQ=255)                                       aaTTCACTGTCGGCAAAGCTAAGTTGAGCACTCCTGATGAACCCTGTTCTATTGCTCCAGATGACAAACATGATCTCATATCAGAGACTTGTTCGCACCTTCCGGAGCCGTTATGAGCTTGCTGCCCTTTTTGTATCTGATTATTAATc              <  1:147872/149‑1 (MQ=18)                                              tGTCGGCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTCTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCAccc       <  1:447556/149‑1 (MQ=255)                                                   gCGAAGATAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGAACAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGAGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAt  >  2:474400/1‑149 (MQ=21)                                                         |                                                                                                                                               AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCGGAGGCGTTATGAGCTGGCGGCCCTTTTTGTATCTGATTATTAATCCCCACCCGCTAT  >  NC_000913_3_pae_tpiA/275009‑275207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑