New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	496998 =	NA (NA)	22 (0.260)	19/224	5.8	NA	intergenic (+4/‑177)	htpG/adk	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
	?	NC_000913_3_pae_tpiA	497030 =	NA (NA)					intergenic (+36/‑145)	htpG/adk	protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase/adenylate kinase
Rejected: Coverage evenness skew score above cutoff.

TGTGTCGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/497155‑496998 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtcatccggcattTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGC  >  NC_000913_3_pae_tpiA/497030‑497178                                                                                                                                                                                                                                                                                                                     TGTGTCGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCAT                                                                                                                                                               <  3:415388/150‑1  GTGTCGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATC                                                                                                                                                              <  1:932693/150‑1    GTCGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCG                                                                                                                                                            <  4:648637/150‑1      CGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGC                                                                                                                                                          <  3:343064/150‑1       GAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCA                                                                                                                                                         <  3:495548/150‑1        AATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCAT                                                                                                                                                        <  4:633823/150‑1           TTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTC                                                                                                                                                                 >  4:412215/1‑138           TTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTC                                                                                                                                                                 <  3:412215/138‑1                             ACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGC                                                                                                                                                          <  2:902240/127‑1                             ACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGC                                                                                                                                                          >  1:902240/1‑127                             ACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGG                                                                                                                                                           >  1:128781/1‑126                             ACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGG                                                                                                                                                           <  2:128781/126‑1                                                                 ACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATC                                                                                                                                                              <  1:217347/87‑1                                                                 ACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATC                                                                                                                                                              >  2:217347/1‑87                                                                                                ATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTT                                                                >  2:390184/1‑150                                                                                                         AAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATC                                                       >  2:570678/1‑150                                                                                                                      GAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATC                                                <  1:708262/144‑1                                                                                                                      GAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATC                                                >  2:708262/1‑144                                                                                                                         AAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATC                                                                                                                    <  1:1091665/73‑1                                                                                                                         AAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATC                                                                                                                    >  2:1091665/1‑73                                                                                                                          AGAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAA                                      <  1:570678/150‑1                                                                                                                           GAAAAATGCCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAA                                     <  1:390184/150‑1                                                                                                                                   CCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTC                               <  2:290124/148‑1                                                                                                                                   CCGGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTC                               >  1:290124/1‑148                                                                                                                                     GGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTA                                                  <  2:997456/127‑1                                                                                                                                     GGATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTA                                                  >  1:997456/1‑127                                                                                                                                      GATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAA                                                                                                          <  4:256025/70‑1                                                                                                                                      GATGACACGAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAA                                                                                                          >  3:256025/1‑70                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTG                                                            >  4:167726/1‑108                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTG                                                            <  3:167726/108‑1                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGA                                                                              >  4:606543/1‑90                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGA                                                                              <  3:606543/90‑1                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACC                                                                                                        >  4:388532/1‑64                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACC                                                                                                        <  3:388532/64‑1                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCC                                                                                                               <  4:538576/57‑1                                                                                                                                              GAAGGTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCC                                                                                                               >  3:538576/1‑57                                                                                                                                                  GTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGT                                                     <  4:547852/111‑1                                                                                                                                                  GTCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGT                                                     >  3:547852/1‑111                                                                                                                                                   aCATCCGGCATTTTTCTTTTCATCATCTGCACTTTCCGCCAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTCGTGGTATCGGTTATCGCTTTTTCAAAAAATTCGACACATGTTAAGGGGAT             >  1:266488/2‑150                                                                                                                                                       CCGGCATTTTTCGCTTCATCTTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTGTCAGCCCCAGGTGCCTTCCTTGAGGCAATCGGCTGTTGGTAGTATCGTTTAGCGCTTTTTCAAATGATTCTACACATTTGAAGGGGATTTTC         <  2:266488/150‑1                                                                                                                                                        CGGCATTTTTTTTTTTTTTTTCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCG        >  2:362089/1‑150                                                                                                                                                        CGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCCAAAAATTCGACACATTTTAATGGGATTTTCG        >  2:405674/1‑150                                                                                                                                                        CGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAA                   <  2:308349/139‑1                                                                                                                                                        CGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAA                   >  1:308349/1‑139                                                                                                                                                        CGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAG                                                                                                 <  2:98387/61‑1                                                                                                                                                        CGGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAG                                                                                                 >  1:98387/1‑61                                                                                                                                                         GGCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGCTTCAGCCCCAGGTGCCTTTCTTCGGGCAATCGCCTGTTGGTGGTATTGTTTATCGCTTTCTCAAAAAATTCCGCACCATTTAAGGTGATTTTCGC       >  2:704086/1‑150                                                                                                                                                          GCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTT                                                                                <  2:10832/76‑1                                                                                                                                                          GCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTT                                                                                >  1:10832/1‑76                                                                                                                                                          GCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTC                                                                                                   >  1:1110275/1‑57                                                                                                                                                          GCATTTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTC                                                                                                   <  2:1110275/57‑1                                                                                                                                                              TTTTTTTTTTATTATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGC  >  4:23830/1‑150                                                                                                                                                                                                                                                                                                                     TGTGTCGAATTTTTTGAAAAAGCGATAAACGATACCACCAACAGGCGATTGCCTCAAGAAAGGCACCTGGGGCTGAAACGGTTAATGGCGAGATAATTTGCGGAAAGTGCAGATGATGAAAAGAAAAATGCCGGATGACACGAAGGTCATCCGGCATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913_3_pae_tpiA/497155‑496998 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtcatccggcattTTTCTTTTCATCATCTGCACTTTCCGCAAATTATCTCGCCATTAACCGTTTCAGCCCCAGGTGCCTTTCTTGAGGCAATCGCCTGTTGGTGGTATCGTTTATCGCTTTTTCAAAAAATTCGACACATTTTAAGGGGATTTTCGCAATGC  >  NC_000913_3_pae_tpiA/497030‑497178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.