Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA3,762,2000AG33.1% 229.6 / 142.2 154A6A (GCA→GCG) rhsARhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (48/55);  new base G (27/24);  total (75/79)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 156 total aligned reads displayed.

ATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTG  >  NC_000913_3_pae_tpiA/3762053‑3762345
                                                                                                                                                   |                                                                                                                                                 
       aTGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAg                                                                                                                                          >  1:696911/1‑150 (MQ=255)
        tGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGCTATCTCACTTCCGGCTTTCTTCTAACTCCGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAACCCGGCAGCGCGTCAgg                                                                                                                                         <  1:110434/150‑1 (MQ=18)
         ggTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAggg                                                                                                                                        <  3:215183/150‑1 (MQ=255)
         ggTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAggg                                                                                                                                        <  4:453476/150‑1 (MQ=255)
          gTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGc                                                                                                                                       <  2:485083/150‑1 (MQ=255)
          gTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGc                                                                                                                                       <  1:1005614/150‑1 (MQ=255)
            gATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGa                                                                                                                                     <  2:105326/150‑1 (MQ=255)
               ataAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACAt                                                                                                                                  >  2:782518/1‑150 (MQ=255)
                  aaTATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGAc                                                                                                                               >  3:592964/1‑150 (MQ=255)
                  aaTATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGACTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGCCAGCGCGTCAGGGCGACATGag                                                                                                                               >  2:756604/1‑149 (MQ=255)
                   atatTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATTAGCGGAAAACCGGCGGCGCGTCa                                                                                                                                           >  4:79736/1‑137 (MQ=255)
                    tatTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGc                                                                                                                             >  1:1154002/1‑150 (MQ=255)
                      ttttCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAg                                                                                                                           <  1:937757/150‑1 (MQ=255)
                            tAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTACTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGc                                                                                                                     >  2:984329/1‑150 (MQ=255)
                             aaTTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAgggt                                                                                                                                       >  3:190248/1‑130 (MQ=255)
                                  tttCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGc                                                                                                               <  2:919146/150‑1 (MQ=255)
                                      aCTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTg                                                                                                           >  1:209921/1‑150 (MQ=255)
                                        tctGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGtt                                                                                                         >  1:324142/1‑150 (MQ=255)
                                          tGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCa                                                                                                       <  1:883409/150‑1 (MQ=255)
                                           gATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAg                                                                                                      <  2:818645/150‑1 (MQ=255)
                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAgg                                                                                                     <  4:184921/150‑1 (MQ=255)
                                            aTGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAgg                                                                                                     >  1:920546/1‑150 (MQ=255)
                                               gATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGtt                                                                                                  <  2:136858/150‑1 (MQ=255)
                                                    ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGcc                                                                                             >  4:266288/1‑150 (MQ=255)
                                                     tcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCg                                                                                            <  3:252422/150‑1 (MQ=255)
                                                      cACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTAt                                                                                                                        <  2:966920/121‑1 (MQ=255)
                                                         ttCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCa                                                                                                                                                   <  4:371577/91‑1 (MQ=255)
                                                         ttCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCa                                                                                                                                                   >  3:371577/1‑91 (MQ=255)
                                                         ttCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCa                                                                                                >  4:6752/1‑142 (MQ=255)
                                                         ttCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt                                                                                        >  1:287567/1‑150 (MQ=255)
                                                           cAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc                                                                                      <  1:647811/150‑1 (MQ=255)
                                                           cAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc                                                                                      <  4:145464/150‑1 (MQ=255)
                                                            aGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGtacg                                                                                     >  1:701022/1‑147 (MQ=16)
                                                            aGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGa                                                                                                                                     >  3:344621/1‑102 (MQ=255)
                                                             ggCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGtacgt                                                                                    >  2:132243/1‑146 (MQ=255)
                                                             ggCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTacgt                                                                                    >  2:132225/1‑149 (MQ=255)
                                                              gCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTg                                                                                       <  4:314211/146‑1 (MQ=255)
                                                               cTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAt                                                                                  <  4:592964/150‑1 (MQ=255)
                                                                tttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCa                                                                                                       >  2:707270/1‑128 (MQ=255)
                                                                tttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCAtt                                                                                 >  1:776499/1‑150 (MQ=255)
                                                                tttCTTATAAATCTGTAGGGTTTCGCCTGGCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCa                                                                                                       <  1:707270/128‑1 (MQ=255)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACATGGATGAGAAATGAGCGGAAAACCGGCGgcgc                                                                                                                                               >  2:16763/1‑82 (MQ=255)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGgcgc                                                                                                                                               <  1:16763/82‑1 (MQ=255)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGcc                                                                           >  4:463759/1‑150 (MQ=11)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGcc                                                                           <  2:904165/150‑1 (MQ=11)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCg                                                                                            >  2:566400/1‑133 (MQ=255)
                                                                      ataAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCg                                                                                            <  1:566400/133‑1 (MQ=255)
                                                                       taAATCTGTAGGGTTTCGCCTGTCAGTAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGccc                                                                          <  4:229710/150‑1 (MQ=255)
                                                                       taAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGccc                                                                          >  2:263103/1‑150 (MQ=11)
                                                                       taAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGccc                                                                          >  1:1107308/1‑150 (MQ=255)
                                                                         aaTCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGccccg                                                                        <  2:223179/150‑2 (MQ=11)
                                                                            cTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGtt                                                                                                  <  1:662342/121‑1 (MQ=255)
                                                                            cTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGtt                                                                                                  >  2:662342/1‑121 (MQ=255)
                                                                            cTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCAcc                                                                     <  3:410151/150‑1 (MQ=255)
                                                                               tAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt                                                                                        <  1:1029141/128‑1 (MQ=255)
                                                                               tAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt                                                                                        >  2:1029141/1‑128 (MQ=255)
                                                                               tAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGc                                                                  >  3:646789/1‑150 (MQ=255)
                                                                                 gggTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGt                                                                <  3:412707/150‑1 (MQ=11)
                                                                                   gTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTgg                                                              >  1:181467/1‑150 (MQ=255)
                                                                                    tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGc                                                             <  1:44548/150‑1 (MQ=11)
                                                                                    tttCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGc                                                             <  3:98452/150‑1 (MQ=255)
                                                                                       cGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTg                                                          <  2:741100/150‑1 (MQ=11)
                                                                                           tGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCg                                                      >  1:535560/1‑150 (MQ=11)
                                                                                             tCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGgt                                                    >  1:708122/1‑150 (MQ=11)
                                                                                              cagcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGgtg                                                   <  3:266288/150‑1 (MQ=255)
                                                                                               agcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGc                                                                                                                                       <  1:737778/65‑1 (MQ=255)
                                                                                               agcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGc                                                                                                                                       >  2:737778/1‑65 (MQ=255)
                                                                                               agcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCggg                                                                                          <  1:786049/110‑1 (MQ=255)
                                                                                                gcagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGtgtg                                                 >  2:331512/1‑150 (MQ=11)
                                                                                                 cagACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGc                                                >  2:363904/1‑150 (MQ=255)
                                                                                                   gACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGccc                                              >  2:692241/1‑150 (MQ=255)
                                                                                                    aCAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGcccc                                             >  4:621427/1‑150 (MQ=255)
                                                                                                    aCAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGcccc                                             >  2:292246/1‑150 (MQ=255)
                                                                                                      aaaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGt                                                                              >  2:924264/1‑115 (MQ=11)
                                                                                                      aaaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCcgg                                           >  2:696844/1‑150 (MQ=11)
                                                                                                      aaaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGt                                                                              <  3:283732/115‑1 (MQ=255)
                                                                                                      aaaTAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGt                                                                              >  4:283732/1‑115 (MQ=255)
                                                                                                          aaCCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGa                                       <  2:281709/150‑1 (MQ=11)
                                                                                                            cccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGt                                                                              <  1:1131196/109‑1 (MQ=255)
                                                                                                            cccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGt                                                                              >  2:1131196/1‑109 (MQ=255)
                                                                                                            cccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGt                                     >  3:443435/1‑150 (MQ=255)
                                                                                                            cccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGt                                     <  4:646789/150‑1 (MQ=255)
                                                                                                            cccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGAGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGt                                     <  1:984329/150‑1 (MQ=255)
                                                                                                             ccGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCg                                                                    <  1:259827/118‑1 (MQ=255)
                                                                                                              cGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCg                                                                                            <  2:706098/93‑1 (MQ=255)
                                                                                                                  aaaaCAAGGATGAGAAATGAGCGGAAAACCGGCGGCGCGTCAGGGTGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTc                               <  3:463759/150‑1 (MQ=1)
                                                                                                                    aaCAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCg                             <  3:93739/150‑1 (MQ=255)
                                                                                                                             gagaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGtt                                                        <  2:225093/114‑1 (MQ=255)
                                                                                                                             gagaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGtt                                                        >  1:225093/1‑114 (MQ=255)
                                                                                                                              agaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGt                   <  2:584826/150‑1 (MQ=255)
                                                                                                                              agaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGt                   <  2:1107308/150‑1 (MQ=255)
                                                                                                                               gaAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTc                  >  2:271255/1‑150 (MQ=255)
                                                                                                                                     aGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTg                                    >  2:302900/1‑126 (MQ=255)
                                                                                                                                      gCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATcccc           <  2:667580/150‑1 (MQ=255)
                                                                                                                                       cGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCt          <  2:776499/150‑1 (MQ=255)
                                                                                                                                         gAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGt                                                                                        >  3:706439/1‑70 (MQ=255)
                                                                                                                                              ccGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTgc                                                                                      >  1:935366/1‑67 (MQ=255)
                                                                                                                                              ccGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCAc                                                                      <  2:839937/83‑1 (MQ=255)
                                                                                                                                               cGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATcc                      <  1:534676/130‑1 (MQ=255)
                                                                                                                                               cGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTg  <  1:271255/150‑1 (MQ=40)
                                                                                                                                                   |                                                                                                                                                 
ATTTATCATGGTGATATAAATATTTTCCTAATTATTTCACTCTGATGGATATCTCACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGAAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGGTTCAGCCGGGGTGCGCATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTG  >  NC_000913_3_pae_tpiA/3762053‑3762345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑