New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_tpiA	= 4040683	31 (1.990)	5 (0.330)	5/244	1.3	16.3%	intergenic (+47/+223)	rrfA/mobB	5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B
	?	NC_000913_3_pae_tpiA	= 4213078	21 (1.370)					intergenic (+3/‑72)	rrfE/yjaA	5S ribosomal RNA of rrnE operon/stress‑induced protein
Rejected: Frequency below/above cutoff threshold.

ATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4040640‑4040683 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCG  <  NC_000913_3_pae_tpiA/4213078‑4212963                                                                                                                                                                  ATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTC                                      <  1:88110/124‑1 ATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTC                                      >  2:88110/1‑124        GAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCAT                                                                   <  3:45250/88‑1        GAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCAT                                                                   >  4:45250/1‑88           AGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGAGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCG  >  3:54350/1‑150                                            TTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG                               >  1:456/1‑88                                            TTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGG                               <  2:456/88‑1                                                                                                                                                                  ATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4040640‑4040683 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCG  <  NC_000913_3_pae_tpiA/4213078‑4212963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.