Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	1,484,228	C→T	Q390* (CAG→TAG)	hrpA →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	1,484,228	0	C	T	100.0%	73.2 / NA	24	Q390* (CAG→TAG)	hrpA	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

TGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGT  >  NC_000913_3_pae_tpiA/1484097‑1484367                                                                                                                                    |                                                                                                                                             tGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAg                                                                                                                             >  1:262984/1‑149 (MQ=255) tGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAg                                                                                                                             >  2:295716/1‑149 (MQ=255)  ggCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTATAAATGTGGAGCCGATTTCCTAGGCGTCTGCCAATCAGc                                                                                                                            <  2:112455/149‑1 (MQ=255)        cAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTCCAGCGTTTGCCGATTGAGCCGATTTACTAGGCGTCTGCCCACCAGCGTAAAg                                                                                                                      >  2:21650/1‑149 (MQ=255)              cgcgGAAACGTCGCTGACCGTACCGGGGATTAAATACGGTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCt                                                                                                                <  2:11911/149‑1 (MQ=255)                                cGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCGGCTGGGGACGTGTGGCCGAAg                                                                                              >  2:93499/1‑149 (MQ=255)                                     cGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATc                                                                                         >  1:58128/1‑149 (MQ=255)                                          aTTAAAGACGTTATCGACCCCGGAACAGCGCGTATCAGCCGCTACAGCCATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAt                                                                                    <  2:338293/149‑1 (MQ=255)                                           ttAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTAtt                                                                                   >  1:263123/1‑149 (MQ=255)                                            tAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGGGTCTGCCAATCAGCGTAAAGGACGCTGTGGTCGTGTGCCCGAAGGGAACTGTATTc                                                                                  >  2:518299/1‑149 (MQ=255)                                                tACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGtct                                                                              <  1:133923/149‑1 (MQ=255)                                                                 tACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGAtt                                                             <  1:310975/149‑1 (MQ=255)                                                                        cgTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCtctc                                                      <  2:204649/149‑1 (MQ=255)                                                                                 cGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGa                                            <  2:402576/149‑1 (MQ=255)                                                                                    tACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGtt                                         >  1:114931/1‑149 (MQ=255)                                                                                             cGCACCAAAGTGCAGCGTTTGCCGATTAAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATcc                                <  1:488076/149‑1 (MQ=255)                                                                                                              tttGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                                                                      <  1:351569/63‑1 (MQ=255)                                                                                                              tttGCCGATTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCgtgt                                                                                                      >  2:351569/1‑63 (MQ=255)                                                                                                                tGCCGATTGACCTGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATGCGTCTCTATTCCGAAGACGATTTCCTTTC‑CGCTCCGGAGTTTATCGATCCGGAGATTCTGCGTACCAAc             <  2:266537/149‑1 (MQ=255)                                                                                                                     aTTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGc‑c                                                <  1:406050/109‑1 (MQ=255)                                                                                                                     aTTGAGCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGc‑c                                                >  2:406050/1‑109 (MQ=255)                                                                                                                          gCCGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATCCGAAGCTTCTGGGTACCAACCTGGCCTCgg   >  2:328497/1‑149 (MQ=255)                                                                                                                           ccGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCGGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGGAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATCCGGAGATCCTGCGTACCAAGCTGGCCTCGGt  >  2:166993/1‑149 (MQ=255)                                                                                                                           ccGATTTCCTAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATCCGGAGATTCTGCCTACCACCCTTGCCTCgct  >  2:164509/1‑147 (MQ=255)                                                                                                                                    |                                                                                                                                             TGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATTAAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTGCAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGTGGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC‑CCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGT  >  NC_000913_3_pae_tpiA/1484097‑1484367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑