New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 411591643 (1.530)4 (0.150) 4/276 3.4 9.0% coding (1223/1509 nt) glpK glycerol kinase
?NC_000913_3_pae_tpiA = 4115921 39 (1.440)coding (1218/1509 nt) glpK glycerol kinase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

AGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4115766‑4115916
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCACCCGCGTTGAGCGCCCGGAAGTGCGCGAAGTCACCGCATTGGGTGCGGCCTATCTCGCAGGCCTGGCGGTTGGCTTCTG  <  NC_000913_3_pae_tpiA/4115921‑4115775
                                                                                                                                                                                                                                                                                                          
AGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCAC                                                                                                                                                       <  1:84107/149‑1
  GTTCTGCCAGAAGCGGAGCGCCGGGCTTGCGGGATAGGCGGCATCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCATCGCGGGTGCCGAGCATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTTCGCCACCATCCACGC                                                                                                                                                     <  2:408291/149‑1
      TGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTCACTTCGCGCACTTCCGGGCGCTCAACGCGGGAGCCGAGAATAACGGACAGGAACCGCCACAGGAAATTGTTTGCTACTGCGCCACCATCCCCGCCCag                                                                                                                                                 >  2:413333/1‑147
                gcACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCCCTGCGCGTGGATG                                                                                                                                       <  2:172289/147‑1
                                                                                     GGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCCCTGCGCGTGGATGGTGGCGCAGTAGCAAA                                                                                                                       >  1:326189/1‑96
                                                                                     GGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGCCCTGCGCGTGGATGGTGGCGCAGTAGCAAA                                                                                                                       <  2:326189/96‑1
                                                                                                                                              tcttCACGCCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCACCCGCGTTGAGCGCCCGGAAGTGCGCGAAGTCACCGCATTGGGTGCGGCCTATCTCGCAGGCCTGGCGGTTG         <  2:298253/145‑1
                                                                                                                                              tccgCACGCCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCACCCGCGTTGAGCGCCCGGAAGTGCGCGAAGTCACCGCATTGGGTGCGGCCTATCTCGCAGGCCTGGCGGTTG         <  1:273733/145‑1
                                                                                                                                                    CGCCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCACCCGCGTTGAGCGCCAAGCAGCCGGCGAAGTCACCGCATTGGGTGCGGCCTATCTCGCAGGCCTGGCGGTTGGCTTCT   <  1:468501/149‑1
                                                                                                                                                     GCCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCCACAGCGTTGAGAGCCCGGAAATGCGCGAAGTCACCACATAGGGTGCGGCCTATCTCGCCGCCCTGGCGGTAGGCTTCTt  >  2:97721/1‑148
                                                                                                                                                                                                                                                                                                          
AGGTTCTGCCAGAAGCCAACCGCCAGGCCTGCGAGATAGGCCGCACCCAATGCGGTGACTTCGCGCACTTCCGGGCGCTCAACGCGGGTGCCGAGAATATCGGACTGGAACTGCATCAGGAAATTGTTTGCTACTGCGCCACCATCCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_tpiA/4115766‑4115916
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcCCTGCGCGTGGATGGTGGCGCAGTAGCAAACAATTTCCTGATGCAGTTCCAGTCCGATATTCTCGGCACCCGCGTTGAGCGCCCGGAAGTGCGCGAAGTCACCGCATTGGGTGCGGCCTATCTCGCAGGCCTGGCGGTTGGCTTCTG  <  NC_000913_3_pae_tpiA/4115921‑4115775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.