Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,110,832 C→T intergenic (+147/+699) cdh → / ← yiiQ CDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,110,8320CT98.5% 460.3 / ‑4.7 131intergenic (+147/+699)cdh/yiiQCDP‑diacylglycerol phosphotidylhydrolase/DUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (63/66);  total (65/66)
Fisher's exact test for biased strand distribution p-value = 2.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

AGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTGGAGACAGCC  >  NC_000913_3_pae_tpiA/4110685‑4110974
                                                                                                                                                   |                                                                                                                                              
aGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTctcc                                                                                                                                              <  2:591294/150‑1 (MQ=255)
     aaGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTg                                                                                                                                         >  1:1223230/1‑150 (MQ=255)
     aaGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTg                                                                                                                                         <  3:143465/150‑1 (MQ=255)
       gagTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGcc                                                                                                                                       <  4:513706/150‑1 (MQ=255)
        agTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCg                                                                                                                                      >  4:592977/1‑150 (MQ=255)
          ttAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAGGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAg                                                                                                                                    >  2:1089876/1‑150 (MQ=255)
             aGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGttt                                                                                                                                 >  1:927107/1‑150 (MQ=255)
               gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCACTTCTAAGACCTTAAGAAATGGGTCCTTTGCCTTCACTTCTTCGCTTGCCAGCAACACCCCCCGCGTTCCGACTTTaa                                                                                                                               >  4:392643/1‑150 (MQ=255)
               gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTaa                                                                                                                               <  3:376665/150‑1 (MQ=255)
               gAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTaa                                                                                                                               <  3:604477/150‑1 (MQ=255)
                 aagtaagtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAgc                                                                                                                             >  1:519407/1‑150 (MQ=255)
                      agtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGc                                                                                                                        >  3:479976/1‑150 (MQ=255)
                       gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCt                                                                                                                       <  3:899368/150‑1 (MQ=255)
                       gtGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCt                                                                                                                       <  1:995611/150‑1 (MQ=255)
                        tGCCGGATTTCATATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCtt                                                                                                                      >  1:498539/1‑150 (MQ=255)
                          ccGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCa                                                                                                                    >  3:755562/1‑150 (MQ=255)
                            ggATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                                                                                                  <  1:440171/150‑1 (MQ=255)
                               tatGAAATCCGGCACCTGTTAGACTTAAGTAGTTCCCTCCCCTTGTATTTCCCCTTATTCTAATTTCTGAAGATATGTGTCTTTTTCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTc                                                                                                               <  1:1062272/150‑1 (MQ=255)
                                   aaaTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCctct                                                                                                           >  1:621178/1‑150 (MQ=255)
                                    aaTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCtctc                                                                                                          <  3:804910/150‑1 (MQ=255)
                                     aTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                                                                                                         >  3:367599/1‑150 (MQ=255)
                                     aTCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTcc                                                                                                         >  3:528206/1‑150 (MQ=255)
                                        cGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGct                                                                                                      >  1:1476962/1‑150 (MQ=255)
                                         ggCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                                                                                                     >  4:21712/1‑150 (MQ=255)
                                            aCCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCa                                                                                                  <  1:45876/150‑1 (MQ=255)
                                              cTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt                                                                                                >  3:819304/1‑150 (MQ=255)
                                                gTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc                                                                                              <  2:621178/150‑1 (MQ=255)
                                                gTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAc                                                                                              <  1:569286/150‑1 (MQ=255)
                                                     aCTTAAGGCGGCCCCTCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCgg                                                                                                                         <  2:1116219/118‑1 (MQ=255)
                                                     aCTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCgg                                                                                                                         >  1:1116219/1‑118 (MQ=255)
                                                     aCTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGcc                                                                                         >  4:835458/1‑150 (MQ=255)
                                                       ttAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                                                                                                    <  1:844188/137‑1 (MQ=255)
                                                       ttAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctct                                                                                                    >  2:844188/1‑137 (MQ=255)
                                                           ggcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtt                                                                                   <  3:679063/150‑1 (MQ=255)
                                                            gcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                                                                                                  <  4:964376/118‑1 (MQ=255)
                                                            gcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAAc                                                                                                                  >  3:964376/1‑118 (MQ=255)
                                                            gcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtta                                                                                  >  2:1457421/1‑150 (MQ=255)
                                                            gcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtta                                                                                  >  3:239453/1‑150 (MQ=255)
                                                            gcggcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATACGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGtta                                                                                  >  1:1483494/1‑150 (MQ=255)
                                                               gcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGc                                                                                          <  3:859844/139‑1 (MQ=255)
                                                               gcCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGc                                                                                          >  4:859844/1‑139 (MQ=255)
                                                                 cccGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACg                                                                             <  2:927107/150‑1 (MQ=255)
                                                                     ccgcGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGa                                                                         <  4:138122/150‑1 (MQ=255)
                                                                     ccgcGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGa                                                                         >  1:646324/1‑150 (MQ=255)
                                                                      cgcgGGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCAAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGAc                                                                        <  4:921874/150‑1 (MQ=255)
                                                                       gcgAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCACTACCCGCCCCAGTTACTACGGCGAcc                                                                       >  4:921736/1‑149 (MQ=255)
                                                                        cgAGCGGGTTCGCCAATTCTAAGATCTTAAGATATGGGGCTTTTGCCTTCACGGCTGCGCTTGCCAGCAAAACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCGCTCAATAGCCTCCCCAGTGATTACGGCGACGt                                                                      >  2:1228544/1‑150 (MQ=255)
                                                                         gAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTc                                                                     >  2:631132/1‑150 (MQ=255)
                                                                          aGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCa                                                                    >  3:336139/1‑150 (MQ=255)
                                                                               tttCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGaa                                                               <  1:631132/150‑1 (MQ=255)
                                                                                   gCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGttt                                                                                                                                 <  4:350433/80‑1 (MQ=255)
                                                                                   gCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGc                                                                                                                        <  3:752723/89‑1 (MQ=255)
                                                                                   gCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGc                                                                                                                        >  4:752723/1‑89 (MQ=255)
                                                                                    ccAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGcc                                                          >  2:797812/1‑150 (MQ=255)
                                                                                      aaTTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTc                                                                                                                     >  3:674401/1‑89 (MQ=255)
                                                                                      aaTTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTc                                                                                                                     <  4:674401/89‑1 (MQ=255)
                                                                                         tCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                                                                                                     >  1:738893/1‑102 (MQ=255)
                                                                                          cTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCa                                                    >  3:998637/1‑150 (MQ=255)
                                                                                          cTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCa                                                    >  3:299275/1‑150 (MQ=255)
                                                                                           tAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCAc                                                   >  1:907968/1‑150 (MQ=255)
                                                                                             aGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTa                                                 <  4:528206/150‑1 (MQ=255)
                                                                                               aTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc                                                                                            >  2:228572/1‑105 (MQ=255)
                                                                                               aTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATAccc                                                                                            <  1:228572/105‑1 (MQ=255)
                                                                                               aTCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGc                                               <  2:519407/150‑1 (MQ=255)
                                                                                                  ttAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTgg                                            >  3:400529/1‑150 (MQ=255)
                                                                                                   tAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGa                                           <  3:592977/150‑1 (MQ=255)
                                                                                                    aaGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt                                                                                                >  3:268793/1‑96 (MQ=255)
                                                                                                    aaGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAAt                                                                                                <  4:268793/96‑1 (MQ=255)
                                                                                                    aaGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGAc                                          >  1:1560900/1‑150 (MQ=255)
                                                                                                      gATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGa                                           <  4:679767/147‑1 (MQ=255)
                                                                                                           gggTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTcc                                   >  2:1611145/1‑150 (MQ=255)
                                                                                                            ggTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCca                                  <  4:400529/150‑1 (MQ=255)
                                                                                                             gTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCcac                                 <  4:755562/150‑1 (MQ=255)
                                                                                                                ttttGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGctc                                                                                                     <  1:908284/79‑1 (MQ=255)
                                                                                                                ttttGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAgg                                                                  <  2:1440343/114‑1 (MQ=255)
                                                                                                                 tttGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCacaaca                             >  4:763410/1‑150 (MQ=255)
                                                                                                                  ttGCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAg                            >  2:1569234/1‑150 (MQ=255)
                                                                                                                    gCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAg                                                                                     >  1:419963/1‑91 (MQ=255)
                                                                                                                    gCCTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAg                                                                                     <  2:419963/91‑1 (MQ=255)
                                                                                                                     ccTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGtcc                         >  2:1596560/1‑149 (MQ=255)
                                                                                                                      cTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGAc                                                                        >  2:853927/1‑102 (MQ=255)
                                                                                                                      cTTCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGtctc                        <  1:1415774/150‑1 (MQ=255)
                                                                                                                       ttCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGttt                                                                                                                                 <  1:1146545/44‑1 (MQ=255)
                                                                                                                       ttCACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGttt                                                                                                                                 >  2:1146545/1‑44 (MQ=255)
                                                                                                                         cACGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCggg                     <  4:860022/150‑1 (MQ=255)
                                                                                                                          aCGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACt                                                  <  1:1290564/120‑1 (MQ=255)
                                                                                                                          aCGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACt                                                  >  2:1290564/1‑120 (MQ=255)
                                                                                                                          aCGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGt                    >  2:1391454/1‑150 (MQ=255)
                                                                                                                           cGGCTGCGCTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGtt                   <  3:835458/150‑1 (MQ=255)
                                                                                                                                gcgcTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATAc              <  2:868014/150‑1 (MQ=255)
                                                                                                                                 cgcTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTGTATACt             >  4:456322/1‑150 (MQ=255)
                                                                                                                                  gcTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACtt            <  1:1391454/150‑1 (MQ=255)
                                                                                                                                   cTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTg           <  4:624898/150‑1 (MQ=255)
                                                                                                                                   cTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTg           >  2:501933/1‑150 (MQ=255)
                                                                                                                                   cTTGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTg           <  2:1476962/150‑1 (MQ=255)
                                                                                                                                    ttGCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTgg          <  2:646324/150‑1 (MQ=255)
                                                                                                                                      gCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGt                                       >  1:333032/1‑119 (MQ=255)
                                                                                                                                      gCCAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGt                                       <  2:333032/119‑1 (MQ=255)
                                                                                                                                       ccAGCAACACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTGgaga       >  1:22396/1‑150 (MQ=255)
                                                                                                                                            aaCACTCTCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTGGAGACAGcc  <  1:797812/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
AGGCGAAGAGTTAAGGAAAGTAAGTGCCGGATATGAAATCCGGCACCTGTCAGACTTAAGGCGGCCCCGCCGCGAGCGGTTTCGCCAATTCTAAGATCTTAAGATATGGGTCTTTTGCCTTCACGGCTGCGCTTGCCAGCAACACTCCCCTCGTGCCGAGTTTAAGCGCGGCTTCAACGTCCTCTCCGCTCTCAATACCCGCCCCAGTTATTACGGCGACGTCAGGGAAGTGCCTCCCCACTAGCTGGACAGTTTCCACAACAGTCTCGGGTTTATACTTGGAGACAGCC  >  NC_000913_3_pae_tpiA/4110685‑4110974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: