Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913_3_pae_tpiA	264,098	G→T	100%	L50L (CTC→CTA)	ykfH ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	264,098	0	G	T	100.0%	124.8 / NA	46	L50L (CTC→CTA)	ykfH	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/20);  total (26/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263952‑264238                                                                                                                                                   |                                                                                                                                             ctgttccccATGAGCATTTTAATCTCCCGCTCCAGGCCCCTGTTAGGTTTTCTCATTACAGTTCTCCACTATTGTCAGTTCATGTTGATGCTCATCAGGCAGGTATAGTGCCCATTGCGGTCCTGGCGGCTTTCGGCGTATACCGCTAg                                                                                                                                            <  2:793416/143‑1 (MQ=255)   ggCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTGCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATGGGGCCCATTGCGGCCCTGGGCGCGGTCGGCGTATACCCCTAGGa                                                                                                                                          >  1:497129/1‑149 (MQ=38)      tCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTc                                                                                                                                       >  1:137291/1‑149 (MQ=255)              gcAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGata                                                                                                                               >  1:448023/1‑149 (MQ=255)                  tgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCg                                                                                                                           >  1:474995/1‑149 (MQ=255)                  tgtAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAACCCGGCGTCGCGGCGATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGCATTCCGGCGCTATCCg                                                                                                                           >  1:461249/1‑149 (MQ=255)                  ggtAAGCTCCCGCTGAAGGCCCCTGGTAGGGTTGCGCATTACAGTGCTCCACCATTGTCAGCTCAGGGTGATGCTCATCAGGCAGGTGTAGGGCCCATTGCGGTCCTGGAGGCGCTCCGCGTATGCCGCTTGGGCTCCGGCCATACCCg                                                                                                                           >  2:230983/2‑149 (MQ=255)                    tAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGa                                                                                                                         >  1:546126/1‑149 (MQ=255)                    tAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGATCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATAGCGCTAGGACTCCGGGGATATCCggc                                                                                                                         >  2:798517/1‑148 (MQ=255)                     aaTCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGaa                                                                                                                        <  1:282830/149‑1 (MQ=255)                      aTCGCCTGCTGCGTGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACGATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAAc                                                                                                                       <  2:724817/149‑1 (MQ=255)                       tctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGCACg                                                                                                                      >  2:620353/1‑149 (MQ=255)                        ctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                                                                                                     >  2:605711/1‑149 (MQ=255)                        ctcCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGt                                                                                                                     >  1:141172/1‑149 (MQ=255)                              cTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGc                                                                                                               <  1:12941/149‑1 (MQ=255)                                  aGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgt                                                                                                           <  2:751580/149‑1 (MQ=255)                                    gCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGgtgt                                                                                                         >  2:483637/1‑149 (MQ=255)                                      cccTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTaa                                                                                                       <  1:526976/149‑1 (MQ=255)                                       catgggtgggTTGCTGATGAGATTTCTCTAGTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATGGCGGTCATGGCGGCGTTCGGCTTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAAt                                                                                                      <  2:3215/142‑1 (MQ=255)                                             aGGGGTGCGCATTACAGTTCTCCACTATTGTCAGTTCAGTGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGa                                                                                                <  2:834871/149‑1 (MQ=255)                                                            aGTTCTCCACTATTGTCAGTCCAGGGTGATGCTACACGACCACGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTCAGAACTCACCCCACCGTGCCACt                                                                                 >  2:205088/1‑149 (MQ=255)                                                                ctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTa                                                                             <  1:440479/149‑1 (MQ=255)                                                                ctcCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTa                                                                             <  1:232688/149‑1 (MQ=255)                                                                  tcACTATTTTCAGTTCAGGGTGATTATCATCAGGCAGTTATAGGGTCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTAtt                                                                           <  2:66734/148‑1 (MQ=18)                                                                      tATTGTCAGTTCAGGGTGATGCCCATCAGGCAGGTATAGCGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGCCCGCCGCAACCGTGCCACTGGTATCTGcc                                                                       >  2:805563/1‑149 (MQ=21)                                                                         tGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGt                                                                    <  1:642512/149‑1 (MQ=255)                                                                         tGTCAGTGCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGTCTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGt                                                                    <  1:586351/149‑1 (MQ=255)                                                                           tCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGc                                                                  >  1:566159/1‑149 (MQ=255)                                                                               ttCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTa                                                              >  1:134760/1‑149 (MQ=255)                                                                                tCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGACCGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAg                                                             >  1:679162/1‑149 (MQ=255)                                                                                            tcatcaGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCAGGTGCAGTAGCGGAAGGTTCgg                                                 >  1:476242/1‑149 (MQ=255)                                                                                              atggggcaggTATATGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGa                                               <  2:81711/145‑1 (MQ=255)                                                                                                   gcaggTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTGCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAg                                          >  1:602117/1‑149 (MQ=255)                                                                                                           aGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTCAGGTACTGCAGGTATTGGCCGGTGCAGTAGCGAAAGATTCGGGAATCAGGATGCTgg                                  >  1:505837/1‑149 (MQ=18)                                                                                                               cccAGTGCGGTCCGGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGt                              <  1:193978/149‑1 (MQ=255)                                                                                                                ccATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGta                             >  1:43653/1‑149 (MQ=255)                                                                                                                   ttGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTatat                          >  2:139110/1‑149 (MQ=255)                                                                                                                    tGCGGTCCTGGCGGCGTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAATTTTCGGGCCTCAGGATGCTGGCGGTATATc                         <  1:483637/149‑1 (MQ=255)                                                                                                                        gTCCTGGCGGGGTTCGGCGTATACCGCTAGGACTTCTGCGATATCCGGAACCTCCCTGCCGGTGTAATGACAGACGCTCACGTCCCACTCGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCa                     <  2:107920/149‑1 (MQ=14)                                                                                                                                 gcgTGCGGCGTATAACGCTAGAGCTCCGGCGAGATCCGGAACGTCCCTGCAGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGc            <  1:845773/149‑1 (MQ=17)                                                                                                                                   gTTCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGACGGTATATCGTAATAGCACTACga          >  1:6518/1‑148 (MQ=17)                                                                                                                                    ttCGGCGTATACCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTAAGGATGCTGGCGGTATATCGTCATTGCCCTGCGtt         >  1:815345/1‑149 (MQ=255)                                                                                                                                       ggCGTATCCGGCTAGGGCTCCGGCGATATCCGGAACGTCCCCGCCAGCTATTTTACCGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTAc      <  1:915093/149‑1 (MQ=14)                                                                                                                                        gcctataCCGGTAGGGCTCCGGCGATATCCGGAACGTCCCGGCCGGTGTAATGGCAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGTGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACt     <  1:350208/146‑1 (MQ=17)                                                                                                                                           tataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCGGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAt  >  2:351407/1‑149 (MQ=255)                                                                                                                                           tataCCGCTAGGACTCCGGCGATATCCGGAACGTCCCTCCCGGGGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATCTCGTCATTGCCCTGCGTTTACTGAt  >  1:35978/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                             CGGGCTCCCAGGCGCAGTGTAATCTCCCGCTGCAGGCCCCTGGTAGGGTTGCTCATTACAGTTCTCCACTATTGTCAGTTCAGGGTGATGCTCATCAGGCAGGTATAGGGCCCATTGCGGTCCTGGCGGCGTTCGGCGTATACCGCGAGGACTCCGGCGATATCCGGAACGTCCCTGCCGGTGTAATGACAGACGCTACCGTGCCACTGGTATTTGCCGGTGCAGTAGCGAAAGATTCGGGACTCAGGATGCTGGCGGTATATCGTCATTGCCCTGCGTTTACTGAT  >  NC_000913_3_pae_tpiA/263952‑264238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑