Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,544,022 1 bp→AA coding (39/1389 nt) narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,544,0211.A76.7% 293.7 / 89.4 103coding (40/1389 nt)narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base . (11/13);  new base A (40/39);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_000913_3_pae_tpiA1,544,0220CA88.2% 285.5 / 30.3 103L13F (TTG→TTT) narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (5/7);  new base A (46/44);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

Only 100 of 111 total aligned reads displayed.

TATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_pae_tpiA/1543877‑1544170
                                                                                                                                                  |||                                                                                                                                                    
tATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑aa‑aa                                                                                                                                                  <  1:1371603/150‑1 (MQ=255)
    gagaTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑A‑aa‑aaagat                                                                                                                                              <  2:1338909/150‑5 (MQ=255)
       aTTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGCAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAAc                                                                                                                                            <  1:978339/150‑1 (MQ=255)
          aaCGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACGGATATCCAGAGAGTTAAACGAGGAATGGGTTATACGAAATGTGCCCAGAAGGCCGGATGTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGAc                                                                                                                                         >  1:1313934/1‑150 (MQ=255)
             ggTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTAt                                                                                                                                      >  1:1528964/1‑150 (MQ=255)
                 aCTGCGCTAAATAGCATCCAGACCCAGAAGACAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTAttttt                                                                                                                                  <  1:433496/150‑1 (MQ=255)
                  cTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTtc                                                                                                                                 <  2:1528964/150‑1 (MQ=255)
                   tGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTtct                                                                                                                                <  1:575794/150‑1 (MQ=255)
                     cgcTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCa                                                                                                                              >  2:379325/1‑150 (MQ=255)
                           aTAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTAGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGc                                                                                                                        <  4:156694/150‑1 (MQ=255)
                                aTCCATACACAAAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAACTATATGTTTTCCTTTATTTTCCCAGAAGTCCGGATTTTCTGGTTTCCAACCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGtaa                                                                                                                   >  4:276400/1‑148 (MQ=255)
                                aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTGATTTTGCAGTGc                                                                                                                   >  3:415284/1‑150 (MQ=255)
                                aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   >  1:1623923/1‑150 (MQ=255)
                                aTCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGc                                                                                                                   >  1:469371/1‑150 (MQ=255)
                                   cAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                >  1:623055/1‑150 (MQ=255)
                                      acacAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATAtt                                                                                                             >  2:774350/1‑150 (MQ=255)
                                       cccaGAAGGCAAGCAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                           <  1:21710/148‑1 (MQ=255)
                                        acaGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                           >  4:905866/1‑150 (MQ=255)
                                         caGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGtt                                                                                                          <  1:610139/150‑1 (MQ=255)
                                          aGAAGGCAAGAAGTAGACACCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCGAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGAATATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         >  2:140606/1‑150 (MQ=255)
                                          aGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTc                                                                                                         <  2:623055/150‑1 (MQ=255)
                                           gAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTACAGTCGAGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTcc                                                                                                        <  4:660444/150‑1 (MQ=255)
                                                 aagaagTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACAt                                                                                                  <  3:399440/150‑1 (MQ=255)
                                                        aGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCAtt                                                                                                                            <  1:1034546/117‑1 (MQ=255)
                                                        aGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCAtt                                                                                                                            >  2:1034546/1‑117 (MQ=255)
                                                         gACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGGTTTCGTTTATTTTCCTAGAAGGCCGGATCTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTTACATGCACACAt                                                                                          >  1:1615050/1‑150 (MQ=255)
                                                          aCAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCNTTATTTTCCCAGAAGGCCGGATCTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATCACGACTATTTTTCTCATTTTGCAGGGCCATATTGTTCCTCACATGCACACAtt                                                                                         >  3:103386/1‑150 (MQ=255)
                                                            aaCTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                       >  2:251926/1‑150 (MQ=255)
                                                               tgactgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTaa                                                                                    >  1:692795/1‑150 (MQ=255)
                                                                 actgaTATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAAc                                                                                                                                            >  1:404690/1‑92 (MQ=255)
                                                                     atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGAc                                                                                                                                         <  1:368076/91‑1 (MQ=255)
                                                                     atatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGAc                                                                                                                                         >  2:368076/1‑91 (MQ=255)
                                                                      tatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑aa‑a                                                                                                                                                   >  1:1433769/1‑79 (MQ=255)
                                                                      tatCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑aa‑a                                                                                                                                                   <  2:1433769/79‑1 (MQ=255)
                                                                       atCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCAtttt                                                                                                                          >  3:119060/1‑104 (MQ=255)
                                                                       atCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCAtttt                                                                                                                          <  4:119060/104‑1 (MQ=255)
                                                                       atCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                            >  3:881445/1‑150 (MQ=255)
                                                                       atCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTCCCCCGGAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaa                                                                            <  1:461776/150‑1 (MQ=255)
                                                                        tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTcac                                                                                                    >  3:931906/1‑125 (MQ=255)
                                                                        tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaaaaa                                                                           >  1:1399000/1‑150 (MQ=255)
                                                                        tCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAg                                                                          >  4:325060/1‑150 (MQ=255)
                                                                          cAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAG‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGa                                                                         <  4:690802/150‑1 (MQ=255)
                                                                           agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCAttt                                                                                                                           >  4:785816/1‑99 (MQ=255)
                                                                           agagATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCAttt                                                                                                                           <  3:785816/99‑1 (MQ=255)
                                                                             agaTTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACaa                                                                      >  1:1268599/1‑150 (MQ=255)
                                                                                tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                     >  1:1003413/1‑116 (MQ=255)
                                                                                tttCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTca                                                                                                     <  2:1003413/116‑1 (MQ=255)
                                                                                   cttcGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACg                                                                <  3:905866/150‑1 (MQ=255)
                                                                                      cGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAgg                                                             >  3:80582/1‑150 (MQ=255)
                                                                                        aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTa                                                           >  1:1560135/1‑150 (MQ=255)
                                                                                        aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑aa‑aa                                                                                                                                                  >  1:652865/1‑62 (MQ=255)
                                                                                        aGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑aa‑aa                                                                                                                                                  <  2:652865/62‑1 (MQ=255)
                                                                                           aaTATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAtt                                                                                         >  1:408334/1‑117 (MQ=255)
                                                                                           aaTATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAtt                                                                                         <  2:408334/117‑1 (MQ=255)
                                                                                            atatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgc                                                       <  2:57841/150‑1 (MQ=255)
                                                                                             tatGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGgcg                                                      >  4:140812/1‑150 (MQ=255)
                                                                                                gTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCaa                                                   <  2:469371/150‑1 (MQ=255)
                                                                                                 ttttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAAt                                                  >  2:1194595/1‑150 (MQ=255)
                                                                                                 ttttCCTTTATTTTCAAGCCAGGCCGGTTTTTCTGGTTCCCCGTCGCG‑C‑AACACGCTAACGACTATTTTTCTCATTTTGCAGTGCAATCTTGTTCATCACATCCGCTCACTGGTAATGAAAAAACGCCAAAACACGAGGTAAGGCGCAAt                                                  <  2:1313934/150‑1 (MQ=255)
                                                                                                  tttCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGACGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATa                                                 <  3:42499/150‑1 (MQ=255)
                                                                                                     ccTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGcc                                              <  1:290213/150‑1 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTtctc                                                                                                                               <  1:136746/68‑1 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGt                                                                                                           <  1:1515106/88‑1 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               <  4:344104/132‑1 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                               <  2:237222/148‑1 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGc                                               >  1:237222/1‑148 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTtctc                                                                                                                               >  2:136746/1‑68 (MQ=255)
                                                                                                      cTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               >  3:344104/1‑132 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCatat                                                                                                              >  1:621808/1‑84 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCatat                                                                                                              <  2:621808/84‑1 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaa                                                                              >  2:1259095/1‑116 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGaaaa                                                                              <  1:1259095/116‑1 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAg                                            >  1:1137498/1‑150 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                       <  4:39415/106‑1 (MQ=255)
                                                                                                       tttATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTgg                                                                                       >  3:39415/1‑106 (MQ=255)
                                                                                                            tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                       >  2:652402/1‑150 (MQ=255)
                                                                                                            tttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatt                                       <  4:80582/150‑1 (MQ=255)
                                                                                                             ttCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatta                                      <  4:179341/150‑1 (MQ=255)
                                                                                                               cccAGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACAATGGAAAAAAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGa                                    <  4:425256/150‑1 (MQ=255)
                                                                                                                  aGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               <  1:1706433/120‑1 (MQ=255)
                                                                                                                  aGAAGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGa                                                               >  2:1706433/1‑120 (MQ=255)
                                                                                                                    aaGGCCGGATTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                               <  2:1623923/150‑1 (MQ=255)
                                                                                                                    aaGGCCGGATTTCTTGGTGTCCAGTCTCC‑AAAA‑AAGATAACGACAATTTTACTCATTTTGCAGTGCAATATTGTCCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTaa                               <  4:415284/150‑1 (MQ=255)
                                                                                                                            aTTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                <  2:757817/61‑1 (MQ=255)
                                                                                                                            aTTTTCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCat                                                                                                                >  1:757817/1‑61 (MQ=255)
                                                                                                                              tttCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTg                     <  1:379325/150‑1 (MQ=255)
                                                                                                                                tCTGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTggg                   >  2:1507277/1‑150 (MQ=255)
                                                                                                                                  tGGTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTTTTTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTg                 >  4:661071/1‑150 (MQ=255)
                                                                                                                                   ggTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGa                <  2:1399000/150‑1 (MQ=255)
                                                                                                                                    gTTTCCAGTCGCGAA‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacac                                                                                            >  3:271871/1‑73 (MQ=255)
                                                                                                                                    gTTTCCAGTCGCG‑AAAA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGcacac                                                                                            <  4:271871/73‑1 (MQ=255)
                                                                                                                                           gTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                       <  3:633148/54‑1 (MQ=255)
                                                                                                                                           gTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCt                                                                                                       >  4:633148/1‑54 (MQ=255)
                                                                                                                                            tCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAtt      <  3:325060/150‑1 (MQ=255)
                                                                                                                                             tcgcgaaaa‑aaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGAt       >  2:1545889/8‑150 (MQ=255)
                                                                                                                                              cgcgaaaa‑aaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTAGGTGAAGTGCTGAtt      <  4:881445/144‑1 (MQ=255)
                                                                                                                                               gcgaaaa‑aaGATAACGACTATTTTTATCATTTTGCAGTGCCATCTCGTTCCTCAAATGCACACATTGGTAATGAAAAAACGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAACTACGGCCCAACTGGGTGCAGTGCCGATTg     >  2:1743791/6‑150 (MQ=255)
                                                                                                                                                 gaaaa‑aaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatta                                      >  3:720587/4‑115 (MQ=255)
                                                                                                                                                 gaaaa‑aaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGttatta                                      <  4:720587/112‑1 (MQ=255)
                                                                                                                                                  aaaa‑aaGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGaaa  <  1:465134/148‑1 (MQ=255)
                                                                                                                                                  |||                                                                                                                                                    
TATTGAGATTAACGGTAACTGCGCTAAATAGCATCCAGACACAGAAGGCAAGAAGTAGACAACTGACTGATATCCAGAGATTTCTTCGAGCAATATGTTTTCCTTTATTTTCCCAGAAGGCCGGATTTTCTGGTTTCCAGTCGCG‑C‑AA‑AAGATAACGACTATTTTTCTCATTTTGCAGTGCCATATTGTTCCTCACATGCACACATTGGTAATGAAAAAAAGACAAAACACGAGGTAAGGCGCAATAGCCAGTTATTAGAATTAAGGATGAATTGGGTGAAGTGCTGATTGAAA  >  NC_000913_3_pae_tpiA/1543877‑1544170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑