New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3305605 =21 (0.730)4 (0.150) 4/272 3.6 15.4% coding (213/1245 nt) mtr tryptophan transporter of high affinity
?NC_000913_3_pae_tpiA 3305636 = 24 (0.880)coding (182/1245 nt) mtr tryptophan transporter of high affinity
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTG  >  NC_000913_3_pae_tpiA/3305461‑3305752
                                                                                                                                                |                                                                                                                                                   
tGCGAAGGCGTGATGCAGAGTCGAACCACTGGCAGAAATATAGGCATACGTCAGGAAATAGAGCCCAAAGGCAATGGACATGCCGTTGACCAGGTTCCAGCCTCTGCACAGCAAATCCTTGCTTGTTGTGTGAAAACTCGAACCGATTc                                                                                                                                                 >  2:427268/1‑149 (MQ=255)
          tgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAg                                                                                                                                       <  1:359090/149‑1 (MQ=255)
                aGAATCGAACCACGAGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGc                                                                                                                                 <  1:46726/149‑1 (MQ=255)
                 gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCt                                                                                                                                >  2:421995/1‑149 (MQ=255)
                          cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAatc                                                                                                                       <  1:475604/149‑1 (MQ=255)
                                                  tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGATTCATCGAGCCGGAATGCAGCATACAg                                                                                               >  2:49180/1‑149 (MQ=255)
                                                  tCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAg                                                                                               <  2:350188/149‑1 (MQ=255)
                                                     ggATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                            <  2:386890/149‑1 (MQ=255)
                                                                acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAACGAt                                                                                 >  2:454142/1‑149 (MQ=255)
                                                                            gAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCAt                                                                     <  1:23151/149‑1 (MQ=255)
                                                                               aTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                            <  1:436148/123‑1 (MQ=255)
                                                                               aTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAAc                                                                                            >  2:436148/1‑123 (MQ=255)
                                                                                 gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAgc                                                                             <  2:70504/136‑1 (MQ=255)
                                                                                 gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAgc                                                                             >  1:70504/1‑136 (MQ=255)
                                                                                          cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAGGATCAGCGCCGCCATTGACCAGaaaaaac                                                       >  1:20429/1‑147 (MQ=255)
                                                                                          cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATACAGGTAAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCCGGTAAAGATCAGCGCCGCCATTGACCAGAAAAAcc                                                       >  1:172074/1‑149 (MQ=255)
                                                                                                                         gTGATGGTGTCAAAACCCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGaaaa                        <  1:331330/149‑1 (MQ=255)
                                                                                                                                        cTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTg                                  >  2:288525/1‑124 (MQ=255)
                                                                                                                                        cTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTg                                  <  1:288525/124‑1 (MQ=255)
                                                                                                                                            aaCCGATTCTGTAATTTAGGTTAGCTTCCAGAAACATCAAGCTGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCTCGGAAATGATCACTGGCAGAGAAAACATCCCCGCGCCATTAATg     <  2:189201/149‑1 (MQ=255)
                                                                                                                                               cGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCGTTGACCAGAAAAACCACGCCCGGGACATGACCACTGGCAGAGAAAACCTCCCTGCGCCCATAATGGtc  >  2:143148/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
TGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAATCATCAAGCCGGAATGCAGCATACAGAACCAGGTAAAGATCAGCGCCGCCATTGACCAGAAAAACCACGCCCCGGACATGACCACTGGCAGAGAAAACATCCCTGCGCCAATAATGGTG  >  NC_000913_3_pae_tpiA/3305461‑3305752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.