New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 3563404 =32 (1.110)4 (0.150) 4/272 3.6 11.7% coding (1392/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
?NC_000913_3_pae_tpiA 3563422 = 30 (1.100)coding (1410/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGA  >  NC_000913_3_pae_tpiA/3563276‑3563568
                                                                                                                                                  |                                                                                                                                                  
ggCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTAATGAGTTCTACGAAGCGGAGCTGAAATACCTGGAGGGACGCGAATGGGTCCGCCGGGCGGACGACGCCCTGTGGCGTCGcac                                                                                                                                                  >  2:17967/1‑149 (MQ=255)
            gAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAt                                                                                                                                      >  2:7571/1‑149 (MQ=255)
                   tgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTa                                                                                                                               >  1:199354/1‑149 (MQ=255)
                   tgctCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCAGGTAGCTa                                                                                                                               >  1:20692/1‑149 (MQ=255)
                        ggCAATGCGGGAACGGTAAGCGATCTCGGGGAAGTTTTCGTTTTCAAGCACTACGAAGCGGGGGTGCAATTTCGTTGGGACCCGGGTTTCGTCGTGTGCTCCTACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGc                                                                                                                          <  1:372826/149‑1 (MQ=255)
                        ggCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCGAAATGc                                                                                                                          >  1:114015/1‑149 (MQ=255)
                                gggAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATcaac                                                                                                                  <  1:7571/149‑1 (MQ=255)
                                       gTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAAtctc                                                                                                            <  1:431251/148‑1 (MQ=255)
                                       gTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAAtctc                                                                                                            >  2:431251/1‑148 (MQ=255)
                                          aGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCgtgt                                                                                                        <  1:125211/149‑1 (MQ=255)
                                                  cGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGt                                                                                                <  2:27540/149‑1 (MQ=255)
                                                      gAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCCCAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCt                                                                                            <  1:234693/149‑1 (MQ=255)
                                                        aGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtgg                                                                                          <  2:150795/149‑1 (MQ=255)
                                                         gATTTGGGGCGTGAGTTCCACGAAGCGGAGCTGAAATACCTGGTGGATCGCGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggt                                                                                         <  2:479415/149‑1 (MQ=255)
                                                         gATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCgt                                                                                                          <  2:313650/132‑1 (MQ=255)
                                                         gATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCgt                                                                                                          >  1:313650/1‑132 (MQ=255)
                                                                       gTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCCAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTTTACgcagc                                                                           >  1:268341/1‑149 (MQ=255)
                                                                              gAAGGGGAGCTAAAATGCCGGGTGGAGCACGAATGGGTCCGCCGCGCCGAGGACGCCCTGTGGCGTCGCAAAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGtt                                                                    <  1:281844/149‑1 (MQ=255)
                                                                                aGCGGAGCTGAAATCCCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGCGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTAt                                                                  <  1:396434/149‑1 (MQ=255)
                                                                                      gCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTgg                                                            >  1:319421/1‑149 (MQ=255)
                                                                                         gAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGcgt                                                         >  1:338141/1‑149 (MQ=255)
                                                                                          aaaTACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGcgtc                                                        <  1:37846/149‑1 (MQ=255)
                                                                                                cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAAt                                                  >  2:403941/1‑149 (MQ=255)
                                                                                                cTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGGTATCGCTGGCGTCGTAAAt                                                  >  2:223332/1‑149 (MQ=255)
                                                                                                                gggTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCa                                  <  2:319421/149‑1 (MQ=255)
                                                                                                                       ccgcgccgACAACGCCTTGTGGCGCCGCACAAAACAAGGGATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa                           <  1:417659/149‑1 (MQ=255)
                                                                                                                              gacgacGCCGTGTGGCGTCGCATAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAAt                    <  2:183683/149‑1 (MQ=255)
                                                                                                                              gacgacGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAAt                    <  2:199354/149‑1 (MQ=255)
                                                                                                                                    gCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGGCGGCTTTCAATCTGGcc              >  2:142185/1‑149 (MQ=255)
                                                                                                                                       cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTga           <  1:403941/149‑1 (MQ=255)
                                                                                                                                             ctgcGCAGAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtga     <  2:278195/146‑1 (MQ=255)
                                                                                                                                                cGCACAAAACGAGGAATGTGGCTAACTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgatga  <  1:408183/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCAGCAACAGCGAGCTGCTGCTCGGCAATGCGGGAACGGTAAGCGATCTCGGGGAAGATTTCGGTCATGAGTTCTACGAAGCGGAGCTGAAATACCTGGTGGATCACGAATGGGTCCGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGA  >  NC_000913_3_pae_tpiA/3563276‑3563568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.