Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_pae_tpiA 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_pae_tpiA 1299499–1300697 1300697 1–1199 36 [0] [0] 36 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA = 12994980 (0.000)35 (1.250) 34/278 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_pae_tpiA 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644
                                                                                                                                                   |                                                                                                                                                  
ttctCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGATTCTCCTCACCATTATGTTATTTTCGCCACCTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                                   >  1:198013‑M1/1‑148 (MQ=255)
   tcAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGTTAGAGGAAAGTGCTAAatat                                                                                                                                                >  2:356971‑M1/1‑145 (MQ=255)
                 gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                                  >  2:137516‑M1/1‑131 (MQ=255)
                  ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTGGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAATTGCTAAataataatcaattgttaaa                                                                                                                                 >  2:185102‑M1/1‑130 (MQ=255)
                    ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                                                                                                                               >  1:22486‑M1/1‑128 (MQ=255)
                       aGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                            <  1:52015‑M1/149‑25 (MQ=255)
                       aGGATTTCCTTAACTGCGTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGACGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTCCTAAataataatcaattgttaaatcatt                                                                                                                            >  2:219399‑M1/1‑125 (MQ=255)
                          aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGATTAATTGAAATGATATTATTAATTACACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattctg                                                                                                                         >  2:162987‑M1/1‑122 (MQ=255)
                          aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                         <  2:4851‑M1/149‑28 (MQ=255)
                                    cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacta                                                                                                               <  1:160589‑M1/149‑38 (MQ=255)
                                      gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                    <  2:71262‑M1/126‑17 (MQ=255)
                                      gCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                                    >  1:71262‑M1/1‑110 (MQ=255)
                                       cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                            <  1:491292‑M1/149‑41 (MQ=255)
                                         tctcCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAACTGATATTATTAATTCCACTGCCTTTGGTATAGGAAAGTGCTAAataataatcaattgttaaattattttgcatttcactactaga                                                                                                          >  2:406615‑M1/1‑107 (MQ=255)
                                                 ccATCATGTTATTGTTGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  <  2:281537‑M1/149‑51 (MQ=255)
                                                  catcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                                 <  1:72439‑M1/149‑52 (MQ=255)
                                                       tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgggactgtaat                                                                                                  <  2:104369‑M1/143‑51 (MQ=255)
                                                       tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                                  >  1:104369‑M1/1‑93 (MQ=255)
                                                         ttATCTTCTCCACATCATAATCTTGGGCTTGCTGAAGAATAATTGAAATGATATTAGTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                          <  2:450305‑M1/149‑59 (MQ=255)
                                                                 gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacat                                                                                  <  2:22486‑M1/149‑67 (MQ=255)
                                                                  ccAAATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATAGTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                                 <  1:382348‑M1/149‑68 (MQ=255)
                                                                                      tGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                             <  2:346970‑M1/149‑88 (MQ=255)
                                                                                         tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggacctgtaatcagaaaagatagacatgcttagccaatctctatttgattg                                                          >  1:167793‑M1/1‑59 (MQ=255)
                                                                                           aagaagaagTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCAAAataataatcaattgttaaattattgtgcaattcactactggaactgtaatcataaaagttagacatgcttagccaatctctatttgatagaa                                                        <  2:463605‑M1/149‑93 (MQ=255)
                                                                                             gaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                      <  1:165827‑M1/149‑95 (MQ=255)
                                                                                              aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgccaatctctatttggttgaattg                                                     >  2:238952‑M1/1‑54 (MQ=255)
                                                                                                  atTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaag                                                 >  1:137903‑M1/1‑50 (MQ=255)
                                                                                                        aagatATTATTATATCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatggtt                                           <  2:63509‑M1/147‑106 (MQ=255)
                                                                                                          gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                         >  2:202729‑M1/1‑42 (MQ=255)
                                                                                                           atatAAATAATTCCACAGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgataaaatattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                                        <  2:3330‑M1/149‑109 (MQ=255)
                                                                                                                attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggc                                   >  2:54074‑M1/1‑36 (MQ=255)
                                                                                                                     ttCCACTGCCTTTAGTAGAGGAAAGTACTAAataataatcaattgttaaagtattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgga                              >  1:414791‑M1/1‑31 (MQ=255)
                                                                                                                           tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                                 <  2:270918‑M1/108‑84 (MQ=255)
                                                                                                                           tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                                 >  1:270918‑M1/1‑25 (MQ=255)
                                                                                                                                      gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttcgccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct             >  1:231540‑M1/1‑14 (MQ=255)
                                                                                                                                                 tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacggtcaa  >  1:147975‑M1/1‑3 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCC  >  NC_000913_3_pae_tpiA/1299351‑1299644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: