Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,296,9501.T20.0% 47.8 / 13.0 20coding (1172/2676 nt)adhEfused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand):  ref base . (6/10);  new base T (2/2);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCG  >  NC_000913_3_pae_tpiA/1296816‑1297089
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tCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGa                                                                                                                                <  1:211247/149‑1 (MQ=255)
     ccAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCACAACCATTTAAGTGGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGaa                                                                                                                           <  1:268627/149‑1 (MQ=255)
         cGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTaa                                                                                                                      <  1:151422/149‑1 (MQ=255)
                       gAGTTACCACCCCAAGTACCACAACCCAGAGTCAGGGCAGGTGCGAGTTTGAAGTTATAGAGGTCACCGATACCACCGTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCgc                                                                                                        <  2:467218/149‑2 (MQ=255)
                                  ccAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCt                                                                                             <  2:9226/149‑1 (MQ=255)
                                      gAACCACAACCCAGAGTCGGGGAAGGTGCGAGTTTGAAGTTATGCATGGCCCAGATCACACGCTCCGAAGCTGGGGTGTTAATCAGGATACGCGCCGTTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGt                                                                                          >  2:360295/1‑149 (MQ=255)
                                      gAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTAACCGATACCACCCTTAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGt                                                                                          >  2:107887/1‑149 (MQ=255)
                                       aaCCACAACCCAGGGTCAGGGAAGGGGCGAGTTTGAAGTTATGCAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCa                                                                                        <  2:128736/149‑1 (MQ=255)
                                          cacaACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACACTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGGGCCGGTTGGTTATCCTGGTCAgtg                                                                                     >  1:214699/1‑149 (MQ=255)
                                                             aGGGGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGAc                                                                  <  2:330459/149‑1 (MQ=255)
                                                                tGCGAGTTTGAAGTTATACAGGCCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGa                                                               <  1:401913/149‑1 (MQ=255)
                                                                                     gTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTc                                                                                                                                         <  1:284264/55‑1 (MQ=255)
                                                                                     gTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTc                                                                                                                                         >  2:284264/1‑55 (MQ=255)
                                                                                          cGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACCGGTAAGAGGGATGACCGATACCGCCCATAGCACCCCGTTTatct                                     >  1:347139/1‑149 (MQ=255)
                                                                                                caccCTGAGACGCTGGGGTGTTAATCGGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATCCCGCCCATAGCAACCAGTTTCTCTGCtttt                               >  2:123273/1‑149 (MQ=255)
                                                                                                     tGAGACGCTGGCGTGTTAATCAGGATACGCGCCGTTTTCATTTTTGTGTGGAGTTAAGCAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGCGGTATGACCGATACCGCCCATACCATCCAGTTTCTCTGCGTTTTTTAc                          <  2:358752/149‑1 (MQ=255)
                                                                                                                        tCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTa       >  1:406775/1‑149 (MQ=255)
                                                                                                                            gATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATAACGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcgc   >  1:478402/1‑149 (MQ=255)
                                                                                                                              tACGCGCCGTTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg  <  1:107887/149‑1 (MQ=255)
                                                                                                                              tACGCGCCGTTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTACGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGcgcg  <  1:360295/149‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                            
TCGGACCAACGTTTTCAGAGATGGAGTTACCACCCCAAGAACCACAACCCAGAGTCAGGGAAGGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCG  >  NC_000913_3_pae_tpiA/1296816‑1297089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: