Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	482,230	G→A	P725L (CCG→CTG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	482,230	0	G	A	100.0%	63.9 / NA	23	P725L (CCG→CTG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAA  >  NC_000913_3_pae_tpiA/482088‑482360                                                                                                                                               |                                                                                                                                   atAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGGTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGCGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAACCTGCAGGCTAt                                                                                                                              >  2:20293/1‑149 (MQ=255) atAAACTTTCTTCACACGACCGCGGTCGATAAAGTAGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTAt                                                                                                                              >  1:425575/1‑149 (MQ=255) atAAACTTTCTTCACACGACCGCGGTCGATAAAATCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTACTGGTCGATATCAATCTTAAACTGCAGGGtgt                                                                                                                              >  1:84846/1‑147 (MQ=255)       gttcttcCGACGCCCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCTTGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCa                                                                                                                        <  2:315590/148‑1 (MQ=255)              acacGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGCCCGtt                                                                                                                 >  2:8746/1‑149 (MQ=255)                            gATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTgg                                                                                                   >  1:485377/1‑149 (MQ=255)                                   tCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTGCTGGTCGATGTCAATCTTAAACTGCAGGGTATCTTCCAGACCGTCTGGACGTACGCTGGTCAAcgt                                                                                            >  2:156512/1‑147 (MQ=255)                                     gTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGACAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACatat                                                                                          <  1:106189/149‑1 (MQ=255)                                      ttCACATAGCTGCCGCCCCATACAGCGCCCAGAGCGGTGTAAGTGTCGTTTATATAAACACCCAGCGGATACGCTTTTTCCGGGTCGCTATCATTCTTAAAATGCAGGAGTTCTTCGCGACCGTTTGGACGTTCGCTCGTAGCCATATc                                                                                         >  2:247084/1‑149 (MQ=255)                                        cacaTAGCTGCTGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAg                                                                                       <  2:36651/149‑1 (MQ=255)                                           aTAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGt                                                                                    >  1:89153/1‑149 (MQ=255)                                                                    ggagTGGTGTTAGTTGCGGTGATAGAAACACCCAGCGCCTGCGCTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGTCGGTTGGGACGGACGCTGGTCAACATAGCAGGGTGCTTCGCTGCTTCTGCAAGCAACTg                                                           <  2:381952/148‑1 (MQ=255)                                                                        tggtggTAACGTCGTTGATAGAAACACCTAGCTCCTGCGCTGTTTCCTGGTCGATAGCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTa                                                       <  1:8746/149‑1 (MQ=255)                                                                                      ttGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATAAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTTCAGGCAACTGGTTACGCGCCTGAGTCAg                                         >  1:56107/1‑149 (MQ=255)                                                                                               acacCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGCGTCAGTTTTTCGTg                                >  2:327671/1‑149 (MQ=255)                                                                                                   ccAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa                            >  1:107753/1‑149 (MQ=255)                                                                                                     aGCGCCTGCGGTGTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAg                          <  2:459043/149‑1 (MQ=255)                                                                                                      gcgcCTGCTCTTTTTCCCGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAgg                         <  1:155354/149‑1 (MQ=255)                                                                                                         ccggcgcTGTTTCTTGGTCGAGATCAATCTCAAACTGCAGGGTATCTTACAGACCGTTTGGACGTACGCTGGCCAACATATCAGGGTGCTTCGCTGCTCCTGCAACCAACTGGTTCCGCGCCTGAGTCAGTTTTTCGTGACCAAGCCCa                      <  2:44416/146‑1 (MQ=255)                                                                                                              gcTTTTTCCTGGTCGATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGGGCCTGAGTCAG‑TTTTCGTGACCAAGGCCAGCCTgg                >  2:494534/1‑149 (MQ=255)                                                                                                                            gATATCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa   >  2:42106/1‑149 (MQ=255)                                                                                                                             atatCAATCTTGAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa  <  2:140333/149‑1 (MQ=255)                                                                                                                             atatCAATCTTAAACTGCAGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa  <  2:321886/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                   ATAAACTTTCTTCACACGACCGCGGTCGATAAAGTCGTTCACATAGCTGCCGCCCCATGCAGCGCCCAGAGTGGTGTTAATGTCGTTGATAGAAACACCCAGCGCCTGCGCTTTTTCCTGGTCGATATCAATCTTAAACTGCGGGGTATCTTCCAGACCGTTTGGACGTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAA  >  NC_000913_3_pae_tpiA/482088‑482360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑