Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA2,255,9910AT20.0% 84.3 / 18.3 40K213* (AAA→TAA) yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (17/15);  new base T (4/4);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAG  >  NC_000913_3_pae_tpiA/2255844‑2256138
                                                                                                                                                   |                                                                                                                                                   
gagTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                    >  1:244949/1‑149 (MQ=255)
 agTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                   <  1:956082/149‑1 (MQ=255)
      ttAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGTCGGTTTATTAATTAAAAGAGAGAGAGTGTATCTCGTGAAGAACAGATAGCAGTTGTCAGGACTGGCGCTGTAGATGGACCCGGAGAATAAATtctc                                                                                                                                              <  2:670478/149‑1 (MQ=255)
       tAGGCGTTTTTTATCGAAATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAACGGGTATCTCATTAAAACCAGCACGCAGATCTCCAGACTCGCGCCGTAGACGAACCCCGAGAACTAATtcttc                                                                                                                                             <  2:751617/149‑3 (MQ=255)
                  tATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCa                                                                                                                                  <  1:362382/149‑1 (MQ=255)
                    tCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCACTTGTCAGGACTGGCGCTGGAGATGGACAAGCAGAATAAATTCTCCTGgatgtttttgt                                                                                                                                <  2:751057/149‑8 (MQ=255)
                         aTCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGACAGCAGTTGTCAGGACTGACGCTGGAGATGGACCCGGAGAATAAATTTTCCGGGATGATGCAGTaaaca                                                                                                                           >  2:70925/1‑147 (MQ=255)
                          tCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCAGGGATGATGCAGTAAAAat                                                                                                                          >  2:698332/1‑149 (MQ=255)
                            ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGAACCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                        >  1:97013/1‑149 (MQ=255)
                            ctttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                        <  1:328468/148‑1 (MQ=255)
                             tttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAattat                                                                                                                       <  1:821397/149‑1 (MQ=255)
                                    gTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAAt                                                                                                                >  2:424256/1‑149 (MQ=255)
                                     ttCTCGCGCAGATCATTCACGACGGTTTATTAATTAAAAGAAAGAAAACGTAGCCCATCAATGTCTTGAAGCACGGGTCAGGACTGGCGATGGAGTTGCGCGGCCAGACTAAATTATCCGGGACGAACCCGTAACACTCATTTTCAATa                                                                                                               >  2:370289/1‑149 (MQ=255)
                                                ttcattcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGcc                                                                                                    >  1:617238/1‑149 (MQ=255)
                                                    ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                                                                <  1:424256/149‑1 (MQ=255)
                                                    ttcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGAt                                                                                                <  1:545063/149‑1 (MQ=255)
                                                                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCg                                                                                                                                            >  2:236110/1‑92 (MQ=255)
                                                                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCg                                                                                                                                            <  1:236110/92‑1 (MQ=255)
                                                                    aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGAAGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                <  1:665871/149‑1 (MQ=255)
                                                                     attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                                                                               <  2:71985/149‑1 (MQ=255)
                                                                     attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCCCCGGGATGATGCAGCAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGATTAATACGta                                                                               >  2:614144/1‑148 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCg                                                                                                                                            >  1:248556/1‑87 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCg                                                                                                                                            <  2:248556/87‑1 (MQ=255)
                                                                         aaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTAAGGGTTTATTCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                                                                           >  1:234708/1‑149 (MQ=255)
                                                                          aaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATc                                                                          >  2:358495/1‑149 (MQ=255)
                                                                                         tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTCCAAGAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                           <  1:541328/149‑1 (MQ=255)
                                                                                             aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAACGCGATTCCCGAATGCAACGCTTAATACGTTTTATCCAGTCTACAAATCGAGCAt                                                       >  2:487552/1‑149 (MQ=255)
                                                                                                aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCTGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                    <  1:766251/149‑1 (MQ=255)
                                                                                                     cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCGTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAg                                               >  2:637778/1‑149 (MQ=255)
                                                                                                                  tCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTcc                                                                                                                                             <  2:982474/42‑1 (MQ=37)
                                                                                                                  tCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTcc                                                                                                                                             >  1:982474/1‑42 (MQ=255)
                                                                                                                  tCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAAc                                  >  1:340158/1‑149 (MQ=255)
                                                                                                                     ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCCTTACGCCAGACCAATAAAGAACCCg                               >  2:77495/1‑149 (MQ=255)
                                                                                                                     ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCg                               <  1:358495/149‑1 (MQ=255)
                                                                                                                     ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCg                               <  2:244949/149‑1 (MQ=255)
                                                                                                                                    atcatcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtc                >  1:759029/6‑149 (MQ=255)
                                                                                                                                        aCCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcatca            >  2:141756/1‑149 (MQ=255)
                                                                                                                                          ccGGAGAATAAATGCTCCGGGAGGAATCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAgg          >  2:730351/1‑149 (MQ=255)
                                                                                                                                           cGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGt                                                                      <  2:278145/88‑1 (MQ=255)
                                                                                                                                           cGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGt                                                                      >  1:278145/1‑88 (MQ=255)
                                                                                                                                              agaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCCATTGCCGGATGCAAGGCCTAATACGTTTTATCCGGTCCACAAATCGAGCATTACTCCAGACCAATAAAGAGCCCGGCAATAGTGGCACTCATCAGGTTgg      <  2:915842/149‑1 (MQ=255)
                                                                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATGGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCCTCAGGTTGGAAAg  >  2:969573/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAG  >  NC_000913_3_pae_tpiA/2255844‑2256138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑