Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,186,4570AG66.5% 185.2 / 151.9 161K398E (AAG→GAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (24/30);  new base G (55/52);  total (79/82)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

CTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATC  >  NC_000913_3_pae_tpiA/4186312‑4186606
                                                                                                                                                 |                                                                                                                                                     
 tCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGaaa                                                                                                                                                  >  3:116648/1‑150 (MQ=255)
    ggTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATg                                                                                                                                               <  1:341930/150‑1 (MQ=255)
       cGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                            <  1:683808/150‑1 (MQ=255)
           cTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAgc                                                                                                                                        >  2:1782597/1‑150 (MQ=255)
            tGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAgcg                                                                                                                                       >  2:1340247/1‑150 (MQ=255)
             gTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAgcgc                                                                                                                                      <  1:1847087/150‑1 (MQ=255)
              tAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGcgcg                                                                                                                                     >  3:162581/1‑150 (MQ=255)
              tAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGcgcg                                                                                                                                     >  1:513798/1‑150 (MQ=255)
                aTCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaa                                                                                                                                   <  4:162581/150‑1 (MQ=255)
                aTCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGtt                                                                                                                                            <  2:1269115/141‑1 (MQ=255)
                   aCCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaagaa                                                                                                                                >  3:1325172/1‑150 (MQ=255)
                    ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGaagaag                                                                                                                               <  2:513798/150‑1 (MQ=255)
                    ccGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGaagaag                                                                                                                               >  4:345190/1‑150 (MQ=255)
                     cGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGc                                                                                                                              <  4:1325172/150‑1 (MQ=255)
                         ggTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTc                                                                                                                          <  1:951516/150‑1 (MQ=255)
                          gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGt                                                                                                                                             <  3:175670/130‑1 (MQ=255)
                          gTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGa                                                                                                                                          >  3:234698/1‑133 (MQ=255)
                            ccATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGtt                                                                                                                       >  4:462581/1‑150 (MQ=255)
                                  cTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGat                                                                                                                 >  4:1276325/1‑150 (MQ=255)
                                    gCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGACGCTGTCGTTTgg                                                                                                                    >  4:213927/1‑145 (MQ=255)
                                     cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                                              <  2:671081/150‑1 (MQ=255)
                                     cGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                                              >  1:671081/1‑150 (MQ=255)
                                         tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                                              >  2:15046/1‑146 (MQ=255)
                                         tGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                                              <  1:15046/146‑1 (MQ=255)
                                          gCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGa                                                                                                         <  3:449674/150‑1 (MQ=255)
                                            aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                    >  2:1784649/1‑137 (MQ=255)
                                            aTCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTgg                                                                                                                    <  1:1784649/137‑1 (MQ=255)
                                             tCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGa                                                                                                      >  1:101233/1‑150 (MQ=255)
                                              cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                     <  3:1276325/150‑1 (MQ=255)
                                              cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                     <  2:1500693/150‑1 (MQ=255)
                                              cAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGaa                                                                                                     >  2:866154/1‑150 (MQ=255)
                                               aGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAg                                                                                                    <  1:866154/150‑1 (MQ=255)
                                                          ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATc                                                                                                              <  1:1798486/129‑1 (MQ=255)
                                                           cGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAac                                                                                        <  1:1969462/150‑1 (MQ=255)
                                                            gaagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCgaa                                                                                                                                   >  1:1355962/1‑106 (MQ=255)
                                                             aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGaaaa                                                                                                                                                 <  2:1599744/91‑1 (MQ=255)
                                                             aagaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGaaaa                                                                                                                                                 >  1:1599744/1‑91 (MQ=255)
                                                              agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATg                                                                                                                                               >  4:1229206/1‑92 (MQ=255)
                                                              agaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATg                                                                                                                                               <  3:1229206/92‑1 (MQ=255)
                                                               gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAg                                                                                                    <  4:113620/134‑1 (MQ=255)
                                                                aaaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                           <  1:641971/110‑1 (MQ=255)
                                                                aaaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGt                                                                                                                           >  2:641971/1‑110 (MQ=255)
                                                                     ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTAct                                                                              >  4:575191/1‑150 (MQ=255)
                                                                     ggCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTAct                                                                              <  3:903489/150‑1 (MQ=255)
                                                                       cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTTAAGCTGCGGTGAAAATGGTTGAGCGCGAAGAACCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCCAACCCTCGGTCctgc                                                                            >  4:615820/1‑150 (MQ=255)
                                                                        agtGGAGCTGTTCAAACCGTTCATCTACGGCCAGCTGGAACCGCGTGGTCTTGCTACCACCATTAAAGCTGCGGCGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           >  2:1205540/3‑150 (MQ=255)
                                                                        aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATg                                                                                                                                               <  4:413274/82‑1 (MQ=255)
                                                                        aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATg                                                                                                                                               >  3:413274/1‑82 (MQ=255)
                                                                        aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           >  3:518049/1‑150 (MQ=255)
                                                                            gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                     <  3:726038/136‑1 (MQ=255)
                                                                            gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACAcc                                                                                     >  4:726038/1‑136 (MQ=255)
                                                                             aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAAcc                                                                      >  1:1705831/1‑150 (MQ=255)
                                                                              gCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCg                                                                     <  3:462581/150‑1 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                            <  4:21421/75‑1 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGtt                                                                                                                                            >  3:21421/1‑75 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                   >  3:243262/1‑100 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                   <  4:243262/100‑1 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                 >  2:984112/1‑150 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                 >  1:1843518/1‑150 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                 >  4:1001476/1‑150 (MQ=255)
                                                                                  ttCAAACCGTTCATCTACGGCAAGCNGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCa                                                                 >  1:285015/1‑150 (MQ=255)
                                                                                   tCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAc                                                                >  1:1819761/1‑150 (MQ=255)
                                                                                     aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTggg                                                                                                                   >  2:739965/1‑97 (MQ=255)
                                                                                          gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGaagaag                                                                                                                               >  1:1274728/1‑80 (MQ=255)
                                                                                            tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           <  3:680260/130‑1 (MQ=255)
                                                                                            tCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           >  4:680260/1‑130 (MQ=255)
                                                                                                    ggCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATg                                                                                                                                               <  4:1217042/54‑1 (MQ=255)
                                                                                                     gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTACCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                              <  1:1158147/150‑1 (MQ=255)
                                                                                                     gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcg                                                                                             >  2:1816428/1‑103 (MQ=255)
                                                                                                          cTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGt                                                                                                                                             <  3:266063/50‑1 (MQ=255)
                                                                                                           tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                        <  2:1843518/150‑1 (MQ=255)
                                                                                                           tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                        >  2:1090448/1‑150 (MQ=255)
                                                                                                           tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                        >  1:1056979/1‑150 (MQ=255)
                                                                                                             gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                                           <  3:1040949/97‑1 (MQ=255)
                                                                                                             gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCcgcg                                                                                           >  4:1040949/1‑97 (MQ=255)
                                                                                                                  gCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           <  2:2135455/108‑1 (MQ=255)
                                                                                                                  gCGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgct                                                                           >  1:2135455/1‑108 (MQ=255)
                                                                                                                   cGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                <  2:1705831/150‑1 (MQ=255)
                                                                                                                   cGTGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCATGCACCGACTCTGCACCGTCTGGGTATCCAGGCAttt                                <  2:887775/150‑1 (MQ=255)
                                                                                                                     tGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                        <  3:732568/140‑1 (MQ=255)
                                                                                                                     tGGTCTTGCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc                                        >  4:732568/1‑140 (MQ=255)
                                                                                                                     tGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGa                              <  3:1291609/150‑1 (MQ=255)
                                                                                                                          ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg                         >  2:471432/1‑150 (MQ=255)
                                                                                                                            gCTACCACCATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTa                       >  4:898570/1‑150 (MQ=255)
                                                                                                                               accaccATTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg                    >  2:1241578/1‑150 (MQ=255)
                                                                                                                                ccaccaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                   <  1:1715217/150‑1 (MQ=255)
                                                                                                                                 caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCACGAACACCCGGTActg                                                                             <  2:1917314/91‑1 (MQ=255)
                                                                                                                                 caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCACGAACACCCGGTActg                                                                             >  1:1917314/1‑91 (MQ=255)
                                                                                                                                  accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                      <  2:2003015/145‑1 (MQ=255)
                                                                                                                                  accaTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc                      >  1:2003015/1‑145 (MQ=255)
                                                                                                                                    caTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa               <  4:626282/150‑1 (MQ=255)
                                                                                                                                     aTTAAAGCTGCGGAGCAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa              <  1:1241578/150‑1 (MQ=255)
                                                                                                                                     aTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGa                                                                                                         >  4:461652/1‑59 (MQ=255)
                                                                                                                                     aTTAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGaa              <  3:1230406/150‑1 (MQ=255)
                                                                                                                                      ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGt                                            >  2:58398/1‑119 (MQ=255)
                                                                                                                                      ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGt                                            <  1:58398/119‑1 (MQ=255)
                                                                                                                                      ttAAAGCTGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg             <  2:1593888/150‑1 (MQ=255)
                                                                                                                                      ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg             <  2:1820488/150‑1 (MQ=255)
                                                                                                                                             tGCGGAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGc      >  1:880348/1‑150 (MQ=255)
                                                                                                                                                 aagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATc  <  1:471432/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CTCCGGTCGTTCTGTAATCACCGTAGGTCCATACCTGCGTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATC  >  NC_000913_3_pae_tpiA/4186312‑4186606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑