Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,197,502 C→T G11R (GGA→AGA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,197,5020CT100.0% 151.6 / NA 42G11R (GGA→AGA) ymfDe14 prophage; putative SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/19);  total (23/19)

ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGA  >  NC_000913_3_pae_tpiA/1197356‑1197646
                                                                                                                                                  |                                                                                                                                                 
aCTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCtgtg                                                                                                                                                <  3:287873/150‑1 (MQ=255)
  tGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCa                                                                                                                                              <  1:31499/150‑1 (MQ=255)
    aTTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATAATCGCACAGATATCTAGACGGCATCGTATGAGGTTTTGCTGCATTTTCTGAGCGAATATTAACTCtgtgtgat                                                                                                                                            >  1:92703/1‑146 (MQ=255)
            atcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt                                                                                                                                    >  1:22497/1‑150 (MQ=255)
            atcaGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt                                                                                                                                    >  2:910079/1‑150 (MQ=255)
            atcaGAATATCTAAGTTTGCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGtt                                                                                                                                    <  1:551667/150‑1 (MQ=255)
                      cTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGt                                                                                                                          <  3:154239/150‑1 (MQ=255)
                       tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatta                                                                                                                                         >  3:382148/1‑134 (MQ=255)
                       tAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatta                                                                                                                                         <  4:382148/134‑1 (MQ=255)
                                 gCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAAtt                                                                                                               >  3:164999/1‑150 (MQ=255)
                                  cAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTa                                                                                                              >  4:405156/1‑150 (MQ=255)
                                       aaaaTCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGtt                                                                                                         >  1:878874/1‑150 (MQ=255)
                                                                    tGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTtttattttat                                                                           <  3:112163/150‑1 (MQ=255)
                                                                          tAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatta                                                                                                                                         >  2:735631/1‑83 (MQ=255)
                                                                          tAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCAtatta                                                                                                                                         <  1:735631/83‑1 (MQ=255)
                                                                          tAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCg                                                                     <  2:47557/150‑1 (MQ=255)
                                                                              ataCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGAt                                                                 >  3:539445/1‑150 (MQ=255)
                                                                               taCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAg                                                                                                >  2:105224/1‑118 (MQ=255)
                                                                               taCTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAg                                                                                                <  1:105224/118‑1 (MQ=255)
                                                                                  tCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                                                                                                            <  2:734532/104‑1 (MQ=255)
                                                                                  tCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAAt                                                                                                            >  1:734532/1‑104 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGc                                                                                                                              <  2:702543/84‑1 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAACAATTCTTTTATTTTATCGTCCGGGATGTTTcc                                                           >  1:213990/1‑150 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTcc                                                           <  2:213990/150‑1 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTcc                                                           <  2:22497/150‑1 (MQ=255)
                                                                                    gCAAAGATATCTAGAGGGCATCGTANGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGc                                                                                                                              >  1:702543/1‑84 (MQ=255)
                                                                                      aaaGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCga                                                         >  2:210300/1‑150 (MQ=255)
                                                                                           tatCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCgacttga                                                    <  4:227239/150‑1 (MQ=255)
                                                                                               tAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACttt                                                <  1:85993/150‑1 (MQ=255)
                                                                                                          gTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGc                                     <  2:756968/150‑1 (MQ=255)
                                                                                                             tGAGGTTTTGCTGCATTCTCTGAGCGGATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCt                                         <  1:826309/143‑1 (MQ=255)
                                                                                                             tGAGGTTTTGCTGCATTCTCTGAGCGGATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCt                                         >  2:826309/1‑143 (MQ=255)
                                                                                                             tGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTa                                  >  3:249849/1‑150 (MQ=255)
                                                                                                               aGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACAATTCAATTAATGTTATT‑TTAAGCGCAAAATAAAATTACTAAAAAGGAAGAGTTAATACCAACTTGACTTTCAGTTCTGGGCTTaca                                >  1:147805/1‑148 (MQ=255)
                                                                                                                     tgctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaa                          <  4:539445/150‑1 (MQ=255)
                                                                                                                       ctgcATTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCTTTGGATTAATGTTATTCTTTTGTGAATGATTTTGTTA‑TTTATCTTCCTGGATTGTGCCGACTTGGCTTTCAGGTCTGGGCTTAAGTGTcagag                        >  1:95186/1‑150 (MQ=255)
                                                                                                                           aTTCTCTGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTcaaagcaaa                    >  2:417775/1‑150 (MQ=255)
                                                                                                                              ctctGAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGat                 <  1:210300/150‑1 (MQ=255)
                                                                                                                                  gAGCGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGataata             >  3:369821/1‑150 (MQ=255)
                                                                                                                                     cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCga                                                         >  2:62574/1‑103 (MQ=255)
                                                                                                                                     cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCga                                                         <  1:62574/103‑1 (MQ=255)
                                                                                                                                     cGAATATTAACTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACca          >  3:542174/1‑150 (MQ=255)
                                                                                                                                             aaCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTga  >  2:481015/1‑150 (MQ=255)
                                                                                                                                             aaCTCTGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTga  >  1:681622/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ACTGATTAATTCATCAGAATATCTAAGTTTTCCGCAGCCAAAATCAAGGGCGTGGCCATTTTTCTCAATGCTTCTAATATACTCGCAAAGATATCTAGAGGGCATCGTATGAGGTTTTGCTGCATTCTCTGAGCGAATATTAACTCCGTGCATATTATAGTTCAAAGCAAGTACCATTCAATTAATGTTATT‑TTTAGTGAAAAATTCTTTTATTTTATCGTCCGGGATTTTTCCGACTTGACTTTCAGTTCTGGGCTTAACTGTCAAAGCAAAGATAATACCACTCACTGA  >  NC_000913_3_pae_tpiA/1197356‑1197646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑