Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,295,9760CT22.1% 390.5 / 102.7 200intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base C (80/75);  new base T (24/20);  total (105/95)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 207 total aligned reads displayed.

GGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913_3_pae_tpiA/4295827‑4296123
                                                                                                                                                      |                                                                                                                                                   
ggccGGGTAGGGCGGTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                     <  2:733619/147‑1 (MQ=255)
      aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                     >  4:216718/1‑144 (MQ=32)
        aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg                                                                                                                                             <  3:212588/150‑1 (MQ=32)
        aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCCACATCAATGCCTGATg                                                                                                                                             <  3:675133/150‑1 (MQ=17)
          ggCGTCTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCg                                                                                                                                           >  4:389560/1‑150 (MQ=17)
           gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTCCGCCAGACAGCACATTAGACTGCTGTGCCG‑CTTCTGGTGAGGGCGGTTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                          >  3:807154/1‑150 (MQ=11)
           gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCCTGATGCATCTACGCCTGATGCGa                                                                                                                                          >  3:650854/1‑150 (MQ=11)
           gCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                          >  3:548423/1‑150 (MQ=32)
           gCGTTGATGTCGGATGCGGCATAAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGa                                                                                                                                          >  2:1109835/1‑150 (MQ=11)
              tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGc                                                                                                                                       >  1:996598/1‑150 (MQ=32)
                 aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                    <  4:802570/150‑1 (MQ=18)
                    ccGCATCTGAGATCAATGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCTAGGGCGCGCAATAGCCTGTTTTCGCGCGATTTTGT‑TCGTCGCATAAGCGGCTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCcg                                                                                                                                 <  2:1361732/150‑1 (MQ=11)
                     cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCcgc                                                                                                                                <  1:942450/150‑1 (MQ=37)
                       cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt                                                                                                                              <  3:355467/150‑1 (MQ=38)
                          ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCtt                                                                                                                           <  4:617369/150‑1 (MQ=21)
                          ccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCCACGCTTGCCGCGTCtt                                                                                                                           >  2:883930/1‑150 (MQ=25)
                           cGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          >  2:310242/1‑150 (MQ=21)
                            gACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                         >  2:1282952/1‑150 (MQ=21)
                             aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                        <  2:996598/150‑1 (MQ=38)
                               aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                      >  4:561047/1‑150 (MQ=25)
                               aTCAACGCCTGATGCGACGCTTAACGCATCTTATCAGGCCTACGCCAGACAGCGCGATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                                                                                      >  1:328745/1‑150 (MQ=255)
                                  aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                   >  2:900416/1‑150 (MQ=21)
                                  aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                   >  2:689843/1‑150 (MQ=21)
                                      ccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACg                                                                                                               >  2:118550/1‑150 (MQ=37)
                                         gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCa                                                                                                            <  4:120243/150‑1 (MQ=39)
                                            gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGcc                                                                                                                                  <  3:333242/125‑1 (MQ=25)
                                            gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAc                                                                                                         >  3:389556/1‑150 (MQ=38)
                                              gACGTTTAATGCGTCTTATCAGGCCTACGCCAGCCCGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCGGGCCTACGCCAGACAg                                                                                                       <  4:650854/150‑1 (MQ=14)
                                               aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAAc                                                                                                                                                     >  1:568727/1‑103 (MQ=32)
                                                 gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                                                                    >  3:232679/1‑150 (MQ=39)
                                                          gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTg                                                                                           >  4:276130/1‑150 (MQ=38)
                                                            cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGcc                                                                                             >  4:686468/1‑146 (MQ=37)
                                                              tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGCCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGAc                                                                                                                                         >  2:35508/1‑100 (MQ=18)
                                                               aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                                 <  2:923334/91‑1 (MQ=34)
                                                               aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCt                                                                                                                                                 >  1:923334/1‑91 (MQ=34)
                                                                tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    >  1:55148/1‑119 (MQ=40)
                                                                tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                                                     >  2:1034890/1‑134 (MQ=40)
                                                                tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                                                     <  1:1034890/134‑1 (MQ=40)
                                                                 cAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    <  4:16013/150‑1 (MQ=38)
                                                                  aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                                                     <  3:698620/132‑1 (MQ=40)
                                                                   ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTg                                                                                                                                    <  1:826299/100‑1 (MQ=21)
                                                                    gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGCGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTg                                                                                 >  4:342642/1‑150 (MQ=255)
                                                                      cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAt                                                                               >  3:604524/1‑150 (MQ=38)
                                                                        aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAttt                                                                             >  1:16664/1‑150 (MQ=39)
                                                                            cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTg                                                                                                                                                <  2:559400/79‑1 (MQ=35)
                                                                            cAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                         >  2:1051910/1‑150 (MQ=37)
                                                                               aCAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                  >  3:529676/1‑122 (MQ=39)
                                                                               aCAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    <  1:627084/136‑1 (MQ=40)
                                                                               aCAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    >  2:627084/1‑136 (MQ=40)
                                                                                cAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                                                                          <  2:1337747/129‑1 (MQ=38)
                                                                                cAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa                                                                                          >  1:1337747/1‑129 (MQ=39)
                                                                                 aGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCCCCCAGACAGCGCAGTAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                    >  1:202480/1‑150 (MQ=255)
                                                                                 aGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAGTAGCCTGATTTAGCGTGATTTTGTAGGGCg                                                                    >  2:851295/1‑150 (MQ=255)
                                                                                  gcgcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGGTGCTATAGTTTTCGTGTCTTATCATGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGTTTTTGTAGGTCgg                                                                   >  1:694211/1‑150 (MQ=11)
                                                                                  gcgcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCgg                                                                   <  1:1051910/150‑1 (MQ=39)
                                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGGc                                                                                                                    >  1:270345/1‑100 (MQ=255)
                                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                    <  2:270345/100‑1 (MQ=255)
                                                                                   cgcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                              <  1:963580/106‑4 (MQ=12)
                                                                                    gcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                        <  2:1311166/95‑1 (MQ=255)
                                                                                    gcAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                        >  1:1311166/1‑95 (MQ=255)
                                                                                      aaTAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                              >  2:599318/1‑87 (MQ=255)
                                                                                      aaTAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGt                                                                                                                              <  1:599318/87‑1 (MQ=255)
                                                                                           ccTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                          >  1:1116880/1‑150 (MQ=38)
                                                                                            cTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGtt                                                         >  1:770038/1‑150 (MQ=39)
                                                                                             tGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                                                        <  2:770038/150‑1 (MQ=38)
                                                                                                     cGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                                                     <  4:448007/97‑1 (MQ=18)
                                                                                                      gTGATTTTGT‑AGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                    <  2:401354/129‑1 (MQ=18)
                                                                                                      gTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg                                                                    >  1:401354/1‑129 (MQ=40)
                                                                                                        gATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          <  4:188697/121‑1 (MQ=40)
                                                                                                          ttttGC‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGa                                           <  4:520318/150‑1 (MQ=37)
                                                                                                            ttGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGACTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                                         >  4:706057/1‑150 (MQ=255)
                                                                                                            ttGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACa                                         >  1:661149/1‑150 (MQ=39)
                                                                                                             tGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc                                                                                    >  4:373029/1‑106 (MQ=40)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                      <  3:442501/86‑1 (MQ=255)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                      >  4:442501/1‑86 (MQ=255)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          <  1:1098101/114‑1 (MQ=255)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          >  2:1098101/1‑114 (MQ=255)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGc                                                 >  2:1224987/1‑139 (MQ=39)
                                                                                                               t‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCa                                      <  2:16664/150‑1 (MQ=38)
                                                                                                                   gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTg                                                                                                                                    >  3:813347/1‑53 (MQ=38)
                                                                                                                   gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGt                                                                          >  1:700920/1‑111 (MQ=40)
                                                                                                                   gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGt                                                          >  4:809931/1‑127 (MQ=255)
                                                                                                                    tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                     >  1:1268410/1‑115 (MQ=255)
                                                                                                                    tCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTc                                                                     <  2:1268410/115‑1 (MQ=18)
                                                                                                                         tacggCGTATACGCCGCATCCGACATTAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                             <  2:124187/147‑1 (MQ=255)
                                                                                                                           aGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                   >  1:897895/1‑94 (MQ=255)
                                                                                                                           aGGCGCATACGCCGCATACGAAATCAACAACTGATGAGAAGCTTGCCGCGTCTTATCAGGCTTACGCCAGCCAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATCAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                           <  2:694211/150‑1 (MQ=11)
                                                                                                                            ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTa                                                                                                                          <  4:542518/54‑1 (MQ=255)
                                                                                                                                tttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                              <  2:492151/78‑1 (MQ=255)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                >  1:463319/1‑71 (MQ=40)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATa                                                                                                <  2:463319/71‑1 (MQ=255)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGc                                                    <  2:946386/115‑1 (MQ=38)
                                                                                                                                     gccgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                              >  2:591260/1‑105 (MQ=255)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa                                                                                                          <  2:972575/56‑1 (MQ=255)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAg                                                                                     <  3:497834/77‑1 (MQ=255)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                    >  2:379502/1‑126 (MQ=37)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc                                    <  1:379502/126‑1 (MQ=37)
                                                                                                                                          aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTc            <  1:118550/150‑1 (MQ=35)
                                                                                                                                                   tatcGCCTGATGTGATGCTTTCGGCGTCTTATCAGGCCTACGCCAGACAGCGCACTCGCCTGTTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATAAGACGCGTTAAGCGTCGCATCAGGc   <  3:15229/147‑1 (MQ=11)
                                                                                                                                                    aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGcc  >  1:916102/1‑150 (MQ=32)
                                                                                                                                                      |                                                                                                                                                   
GGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGT‑AGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCC  >  NC_000913_3_pae_tpiA/4295827‑4296123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑