| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_tpiA | 1,250,961 | 1 | . | C | 26.7% | 27.9 / 15.4 | 15 | intergenic (‑123/‑105) | dhaK/dhaR | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit/dhaKLM operon transcription activator |
| Reads supporting (aligned to +/- strand): ref base . (6/5); new base C (2/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTT > NC_000913_3_pae_tpiA/1250830‑1251109 | ttttttCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACataatttgg < 2:19917/150‑7 (MQ=255) tAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATAGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACgtg > 1:87282/1‑150 (MQ=255) gCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCGGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTAtt > 3:60972/1‑150 (MQ=255) aCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCACTA‑TGAAACataatt > 1:72650/1‑93 (MQ=255) aCGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCACTA‑TGAAACataatt < 2:72650/96‑4 (MQ=255) cGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGa < 2:58935/150‑1 (MQ=255) ggCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGa > 1:30952/1‑150 (MQ=255) ggCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGa > 1:46676/1‑150 (MQ=255) aTGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAgg > 1:102642/1‑150 (MQ=255) tgCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGatat < 2:102642/150‑1 (MQ=255) gCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGgcg < 2:30952/150‑1 (MQ=255) taaagcatATGTTTCACTA‑TGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgg > 4:64304/8‑150 (MQ=255) aagcatATGTTTCACTA‑TGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCgggg < 3:64304/145‑1 (MQ=255) aaTTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCa > 4:20024/1‑150 (MQ=255) cA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCtt < 4:60972/150‑1 (MQ=255) | TTTTTTCATTATTTTGCTCCAGCAATTACGGTAGGGCATGGATGATGTTCAACGACACGGCGACCACTGACTGCCGATGAATCCATTGTGCATCAGGCAAGGGAAAAGAAAATTAGCCCAAATTATGTTTCA‑TAGTGAAACATATGCTTTAATGAATGTTCCATATTGAAACTTTTACGTGTATTAATACTTAAAATTGCGAGCCGGAACACCTTTTGTCATAAGGGATGCGGGATATGAGTGGCGCTTTTAACAACGATGGTCGGGGCATATCTCCCTT > NC_000913_3_pae_tpiA/1250830‑1251109 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |