Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	4,367,616	3 bp→TCC	coding (626‑628/1437 nt)	aspA ←	aspartate ammonia‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	4,367,616	0	G	T	100.0%	12.6 / NA	5	L210M (CTG→ATG)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (2/3);  total (2/3)
*	NC_000913_3_pae_tpiA	4,367,617	0	G	C	100.0%	12.1 / NA	5	I209R (ATC→AGG)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (2/3);  total (2/3)
*	NC_000913_3_pae_tpiA	4,367,618	0	A	C	100.0%	12.4 / NA	5	I209R (ATC→AGG)	aspA	aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/3);  total (2/3)

CAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGC  >  NC_000913_3_pae_tpiA/4367481‑4367630                                                                                                                                        |||             cAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaag  <  1:48039/150‑4 (MQ=255)                cTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAga    >  1:123864/1‑132 (MQ=255)                cTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAga    <  2:123864/133‑2 (MQ=255)                                        gCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaa   >  1:95656/1‑108 (MQ=255)                                        gCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCATCCTGCTGAAAgaa   <  2:95656/110‑3 (MQ=255)                                                                                                                                        |||             CAGCCAGTTTTTTCACTGCCAGCGGAGAGTACTCTTTCGGCGTGTTCAGACCAGTACCGATTGCTGTTGCACCAAGGTTAACTTCCAGCAGCAGTTCAGCGGTACGTTGGATGTTTTTCACTTCTTCTTTCAGCAGGATGCTGAAAGCGC  >  NC_000913_3_pae_tpiA/4367481‑4367630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑