Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 1,756,829 Δ1 bp coding (1132/1413 nt) pykF → pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA1,756,8290A.92.6% 381.7 / 28.8 96coding (1132/1413 nt)pykFpyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base A (4/3);  new base . (47/41);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.01e-01

GGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTC  >  NC_000913_3_pae_tpiA/1756681‑1756971
                                                                                                                                                    |                                                                                                                                              
ggCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTAc                                                                                                                                               >  1:857098/1‑150 (MQ=255)
     ccATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAggg                                                                                                                                         <  1:531751/150‑1 (MQ=255)
         cTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGt                                                                                                                                     <  1:573882/150‑1 (MQ=255)
         cTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGt                                                                                                                                     <  3:713750/150‑1 (MQ=255)
          tGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGAGCATTACAGAAAAGCTCTGCCAACGTGCAGTTGGATATGCTGCAAAACTGGATGATTCGCTGATCGTGGTTGCT‑CTCAGGGCggcc                                                                                                                                    >  3:906460/1‑148 (MQ=255)
            cGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTcaa                                                                                                                                  >  1:1072892/1‑150 (MQ=255)
            cGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTaaa                                                                                                                                  >  1:1065946/1‑150 (MQ=255)
            cGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTaaa                                                                                                                                  >  2:306451/1‑150 (MQ=255)
             gAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTaaa                                                                                                                                  <  2:857098/150‑1 (MQ=255)
             gAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAAt                                                                                                                                 >  2:558679/1‑150 (MQ=255)
               aCGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCt                                                                                                                               >  4:825004/1‑150 (MQ=255)
                 gTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGc                                                                                                                             >  4:608663/1‑150 (MQ=255)
                 gTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGc                                                                                                                             <  1:269988/150‑1 (MQ=255)
                   accgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAGACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTc                                                                                                                           <  1:912476/150‑1 (MQ=255)
                     cgaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTcgc                                                                                                                         <  2:1353526/150‑1 (MQ=255)
                      gaccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTcgcg                                                                                                                        <  1:287462/150‑1 (MQ=255)
                       accgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACGACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCgcgc                                                                                                                       >  2:1305468/1‑150 (MQ=255)
                       accgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCgcgc                                                                                                                       >  2:911146/1‑150 (MQ=255)
                        ccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCa                                                                                                                      <  2:1268505/150‑1 (MQ=255)
                        ccgCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCa                                                                                                                      <  1:1015293/150‑1 (MQ=255)
                          gcgTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGt                                                                                                                    <  4:295768/150‑1 (MQ=255)
                            gTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTAc                                                                                                                  <  3:63862/150‑1 (MQ=255)
                            gTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTAc                                                                                                                  >  3:289486/1‑150 (MQ=255)
                                  aaCAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAAt                                                                                                            >  1:143442/1‑150 (MQ=255)
                                       ccGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAACACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGt                                                                                                                    <  3:426314/137‑1 (MQ=255)
                                       ccGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGAGGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGt                                                                                                                    >  4:426314/1‑137 (MQ=255)
                                        cGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTcc                                                                                                      <  2:398211/150‑1 (MQ=255)
                                            tcGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGa                                                                                                  >  2:14009/1‑150 (MQ=255)
                                            tcGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGa                                                                                                  >  1:132985/1‑150 (MQ=255)
                                              gAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATg                                                                                                >  1:666863/1‑150 (MQ=255)
                                              gAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATg                                                                                                >  2:1026759/1‑150 (MQ=255)
                                                 ttCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGcca                                                                                             <  1:1254121/150‑1 (MQ=255)
                                                 ttCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCGCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGcca                                                                                             >  2:831259/1‑150 (MQ=255)
                                                  tCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGccac                                                                                            >  1:847503/1‑150 (MQ=255)
                                                   caacaaTGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGccacc                                                                                           >  1:638030/1‑150 (MQ=255)
                                                    aacaaTGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGccacca                                                                                          >  1:108803/1‑150 (MQ=255)
                                                      caaTGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATc                                                                                        <  1:911146/150‑1 (MQ=255)
                                                       aaTGACAACCGTAAACTGCGCATTACCGAAGCGGGATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATcc                                                                                       >  1:710715/1‑150 (MQ=255)
                                                         tGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTg                                                                                     <  3:374767/150‑1 (MQ=255)
                                                         tGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTg                                                                                     <  4:731605/150‑1 (MQ=255)
                                                             aaCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTcgcg                                                                                                                        >  3:285276/1‑111 (MQ=255)
                                                             aaCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTcgcg                                                                                                                        <  4:285276/111‑1 (MQ=255)
                                                               ccGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTg                                                                               >  1:74812/1‑150 (MQ=255)
                                                                   aaaCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGacca                                                                           <  2:638030/150‑1 (MQ=255)
                                                                       tGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCaa                                                                       >  2:886247/1‑150 (MQ=255)
                                                                            aTTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGaaa                                                                  >  1:1264498/1‑150 (MQ=255)
                                                                            aTTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGaaa                                                                  <  3:608663/150‑1 (MQ=255)
                                                                                ccGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTaaa                                                                                                             >  2:144938/1‑103 (MQ=255)
                                                                                ccGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTaaa                                                                                                             <  1:144938/103‑1 (MQ=255)
                                                                                 cGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACg                                                             >  3:701364/1‑150 (MQ=255)
                                                                                  gAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACgg                                                            >  1:1024124/1‑150 (MQ=255)
                                                                                  gAAGCGGTATGCCGTGGTGCCATTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACgg                                                            <  2:44371/150‑1 (MQ=255)
                                                                                   aaGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGc                                                           >  2:180376/1‑150 (MQ=255)
                                                                                   aaGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGc                                                           <  1:918062/150‑1 (MQ=255)
                                                                                    aGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCt                                                          >  4:377716/1‑150 (MQ=255)
                                                                                       ggTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCtcat                                                       <  4:702742/150‑1 (MQ=255)
                                                                                            gCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGcc                                                                                              >  1:434676/1‑106 (MQ=255)
                                                                                            gCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGcc                                                                                              <  2:434676/106‑1 (MQ=255)
                                                                                                 ggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTAc                                             >  2:483257/1‑150 (MQ=255)
                                                                                                  gtgCCGTTGACACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTGGCT‑CTCAGGGCGGTAAACCGGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACt                                            <  2:605531/150‑1 (MQ=255)
                                                                                                  gtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACt                                            >  3:848618/1‑150 (MQ=255)
                                                                                                   tgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTg                                           >  2:1003298/1‑150 (MQ=255)
                                                                                                    gCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGa                                          >  1:396705/1‑150 (MQ=255)
                                                                                                    gCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGa                                          >  1:569230/1‑150 (MQ=255)
                                                                                                    gCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGa                                          >  1:1461744/1‑150 (MQ=255)
                                                                                                      cGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACAAAAAAAAAAACGGCTCATCAGTTGGTACTGAGc                                        <  2:79168/150‑1 (MQ=255)
                                                                                                       gTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCa                                                                                  <  1:1293342/107‑1 (MQ=255)
                                                                                                       gTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCa                                                                                  >  2:1293342/1‑107 (MQ=255)
                                                                                                       gTTGAAACTGCTGAAAAACAGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCCCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGCCCACCAACGAAAAAACGGCTCATCAGTAGGTCATGAGc                                        >  2:840566/1‑150 (MQ=255)
                                                                                                         tGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCaaa                                     <  1:360077/150‑1 (MQ=255)
                                                                                                            aaCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGc                                  <  4:701364/150‑1 (MQ=255)
                                                                                                              gtgctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTaa                                                                                                              >  4:302666/2‑72 (MQ=255)
                                                                                                              gtgctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTaa                                                                                                              <  3:302666/71‑1 (MQ=255)
                                                                                                               tgctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGtt                               <  1:944931/150‑1 (MQ=255)
                                                                                                               tgctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGtt                               <  3:377716/150‑1 (MQ=255)
                                                                                                                gctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtg                              >  3:616521/1‑150 (MQ=255)
                                                                                                                  tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtgtg                            >  2:581350/1‑150 (MQ=255)
                                                                                                                  tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtgtg                            >  2:479066/1‑150 (MQ=255)
                                                                                                                  tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCAGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACAATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTGTGTACTGAGCAAAGGCGTtgtg                            >  2:1461963/1‑150 (MQ=255)
                                                                                                                    aaaaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgcg                                                                                                                        <  1:1129328/57‑1 (MQ=255)
                                                                                                                    aaaaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTcgcg                                                                                                                        >  2:1129328/1‑57 (MQ=255)
                                                                                                                    aaaaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGc                           >  1:985947/1‑150 (MQ=255)
                                                                                                                    aaaaaCTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGcc                          >  1:448086/1‑150 (MQ=255)
                                                                                                                    aaaaaCTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGcc                          >  4:57638/1‑150 (MQ=255)
                                                                                                                     aaaaCTGGATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCg                         <  2:1259290/150‑1 (MQ=255)
                                                                                                                           ggATGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCtt                   <  2:627635/150‑1 (MQ=255)
                                                                                                                             gggCTCCGCTGAGCGTGGGTG‑AGCGCAGGGCGGTAAATCTGAGCGCGCAGGACGGAAAGAGTTCCCGGATGCCACCAGCATGGCACTGAAAAAAAAAAAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGt                 <  2:1342457/148‑1 (MQ=255)
                                                                                                                             aTGCTCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGt                 <  2:1065946/150‑1 (MQ=255)
                                                                                                                                 tCCGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaaa             >  2:86747/1‑150 (MQ=255)
                                                                                                                                   cGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga           <  1:558679/150‑1 (MQ=255)
                                                                                                                                   cGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga           <  2:1072892/150‑1 (MQ=255)
                                                                                                                                   cGCTGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga           <  1:306451/150‑1 (MQ=255)
                                                                                                                                    gCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga           <  2:985947/150‑1 (MQ=255)
                                                                                                                                      tGATCGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAt        <  4:348503/150‑1 (MQ=255)
                                                                                                                                          cGTGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACt    >  1:853836/1‑150 (MQ=255)
                                                                                                                                            tGGTTGCT‑CTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTc  <  2:658885/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GGCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTC  >  NC_000913_3_pae_tpiA/1756681‑1756971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑