Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,111,596 G→A L179L (CTG→TTG)  yiiQ ← DUF1454 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,111,5960GA98.7% 245.3 / NA 74L179L (CTG→TTG) yiiQDUF1454 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (34/39);  total (34/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_pae_tpiA/4111450‑4111740
                                                                                                                                                   |                                                                                                                                                
tAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCTAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAccc                                                                                                                                               <  2:721180/150‑1 (MQ=255)
 aaaGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAAcccc                                                                                                                                              <  3:227333/150‑1 (MQ=255)
   aGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCtt                                                                                                                                            >  2:655510/1‑150 (MQ=255)
              cgccCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGt                                                                                                                                 >  1:935810/1‑150 (MQ=255)
                  ccGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCcgc                                                                                                                             <  1:830930/150‑1 (MQ=255)
                              agGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGTTTAA‑GATGGCGCCAGCTTAATCGATTCAAGAGCGAAGGTCAACGCCTTTTCGCCGTTGTCCGCGGAAACATAACg                                                                                                                 >  1:840853/1‑150 (MQ=255)
                                gTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               <  2:997075/150‑1 (MQ=255)
                                  cTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAgg                                                                                                             >  2:839813/1‑149 (MQ=255)
                                   tgtgAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                            <  2:709326/150‑1 (MQ=255)
                                       aaaCAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCAcc                                                                                                        <  1:78917/150‑1 (MQ=255)
                                          cAGTATAGAGATTCATCGGAACAAAGGCTTTGCTTTTTGTCATATATTCAACACTTATCGCGATTCACAACAAAACCA‑CGTAATCGGTCCACCCGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTc                                                                                                     <  1:668565/150‑1 (MQ=255)
                                              ataGAGATTCATCGGCACAAAGGCTTTGCTTTTTTTTTTTTTTTTTAACCTTCAAGCGATTCA‑GATAGCTCCAGCTTTGTCGGTTCAACAGCGAAGGTCAACCCCTTTTCTCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                                                                                                 >  4:93900/1‑150 (MQ=255)
                                              ataGAGAGTCATCGGCACGAAGGCTTTGCGTTGTGTCATTTATTCAGACCTTCGAGCGATTCA‑GATAGCGCCAGCGTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtc                                                                                                 <  2:1084759/150‑1 (MQ=255)
                                                agagATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtctc                                                                                               <  3:467118/150‑1 (MQ=255)
                                                   gaTTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGgt                                                                                            <  4:281911/150‑1 (MQ=255)
                                                      tCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGta                                                                                         <  1:972404/150‑1 (MQ=255)
                                                        aTCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGtaat                                                                                       >  3:263832/1‑150 (MQ=255)
                                                         tCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGtaata                                                                                      >  3:687424/1‑150 (MQ=255)
                                                              acaAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACgtt                                                                                 >  1:630553/1‑150 (MQ=255)
                                                                 aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                            <  1:1097503/120‑1 (MQ=255)
                                                                 aaGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTg                                                                                                            >  2:1097503/1‑120 (MQ=255)
                                                                      tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTc                                                                         >  1:119172/1‑150 (MQ=255)
                                                                      tttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTc                                                                         >  3:225816/1‑150 (MQ=255)
                                                                       ttgctttTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcc                                                                        <  1:825401/150‑1 (MQ=255)
                                                                         gttttttGTCATTTATTCAAACCTTCAAGCGAGTCA‑GATAGCGCCATCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTcccc                                                                      <  2:462580/148‑1 (MQ=255)
                                                                           tttttGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgc                                                                    <  1:901333/150‑1 (MQ=255)
                                                                             tttGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               >  4:346836/1‑105 (MQ=255)
                                                                             tttGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCa                                                                                                               <  3:346836/105‑1 (MQ=255)
                                                                               tGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGtt                                                                <  1:1081885/150‑1 (MQ=255)
                                                                               tGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGtt                                                                >  2:522058/1‑150 (MQ=255)
                                                                                     ttATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt                                                                 <  3:239618/143‑1 (MQ=255)
                                                                                     ttATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt                                                                 <  3:239867/143‑1 (MQ=255)
                                                                                     ttATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt                                                                 >  4:239867/1‑143 (MQ=255)
                                                                                     ttATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGt                                                                 >  4:239618/1‑143 (MQ=255)
                                                                                        ttCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                       >  1:508734/1‑150 (MQ=255)
                                                                                           aaaCCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc                                                    >  1:221706/1‑150 (MQ=255)
                                                                                           aaaCCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGc                                                    >  3:525446/1‑150 (MQ=255)
                                                                                              ccTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCATTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGGTAGTAGCGACTGCAGt                                                 >  3:243247/1‑150 (MQ=255)
                                                                                               cTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAc                                                                                    <  3:274117/114‑1 (MQ=255)
                                                                                               cTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAAc                                                                                    >  4:274117/1‑114 (MQ=255)
                                                                                                 tCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGtttt                                              <  3:80919/150‑1 (MQ=255)
                                                                                                     ggggttCA‑GGTATCGCGAGTTTACTCGGTTCAACAGTGAAGGTCTACCCCTTTTCGCCGTTGTCCGCGACATCATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCt                                          <  2:948556/146‑1 (MQ=255)
                                                                                                     gCGATTCC‑GATAGCGCCAGCTTAATCGGTTCAACCGCGAAGGTCAACCCTTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCt                                          <  4:687424/150‑1 (MQ=255)
                                                                                                     gCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                           >  1:146799/1‑69 (MQ=255)
                                                                                                     gCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGa                                                                                                                           <  2:146799/69‑1 (MQ=255)
                                                                                                           cA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                                    <  1:522058/150‑1 (MQ=255)
                                                                                                            a‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtg                                   >  4:33186/1‑150 (MQ=255)
                                                                                                                 aGCGCCAGCTTAATCGCTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTACCGACTGCAGTTTTTTCTGGCTTTGTGtt                               >  3:614359/1‑150 (MQ=255)
                                                                                                                    gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            <  3:85314/150‑1 (MQ=255)
                                                                                                                    gcCAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGGTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTg                            >  1:1063952/1‑150 (MQ=255)
                                                                                                                     ccAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTCTGTGTTTTgg                           >  2:789824/1‑150 (MQ=255)
                                                                                                                      cAGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                          <  4:225816/150‑1 (MQ=255)
                                                                                                                       aGCTTAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTc                         <  4:525446/150‑1 (MQ=255)
                                                                                                                         cTTAATCGGTTCAACAGCGTAGGTCAACCCGTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                       <  1:721594/150‑1 (MQ=255)
                                                                                                                          ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtct                                                                                                <  4:49732/76‑1 (MQ=255)
                                                                                                                          ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGtct                                                                                                >  3:49732/1‑76 (MQ=255)
                                                                                                                          ttAATCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAtt                      <  4:263832/150‑1 (MQ=255)
                                                                                                                            aaTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTcc                                                                                                                               >  2:151919/1‑43 (MQ=255)
                                                                                                                            aaTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTcc                                                                                                                               <  1:151919/43‑1 (MQ=255)
                                                                                                                             aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                  >  2:41533/1‑135 (MQ=255)
                                                                                                                             aTCGGTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                  <  1:41533/135‑1 (MQ=255)
                                                                                                                                ggTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                <  2:935810/150‑1 (MQ=255)
                                                                                                                                ggTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                >  1:777727/1‑150 (MQ=255)
                                                                                                                                ggTGCAACAGCGAAGGTCTACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCTCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                <  4:346373/150‑1 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACGACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgccg                                                                  <  1:659972/99‑1 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccgccg                                                                  >  2:659972/1‑99 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGTCTTTGTGTTTTGGTCATTAATgggg               >  4:373226/1‑150 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATgggg               >  3:165080/1‑150 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATg                  <  2:547225/147‑1 (MQ=255)
                                                                                                                                 gTTCAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATg                  >  1:547225/1‑147 (MQ=255)
                                                                                                                                    cAACAGCGAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa            >  2:887972/1‑150 (MQ=255)
                                                                                                                                          cGAAAGTCAGCACCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc      <  2:290541/150‑1 (MQ=255)
                                                                                                                                           gAAGGTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCg     <  1:887972/150‑1 (MQ=255)
                                                                                                                                              ggTCAACCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAt  <  2:1063952/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TAAAGGAAGATGGCCGCCCCGCAGGGCAGCAGGTCTGTGAAACAGTATAGAGATTCATCGGCACAAAGGCTTTGCTTTTTGTCATTTATTCAAACCTTCAAGCGATTCA‑GATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGAT  >  NC_000913_3_pae_tpiA/4111450‑4111740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑