New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_tpiA 1570598 =53 (1.330)4 (0.110)
+CCGTTACCGTTAAAC
4/248 5.0 10.0% intergenic (‑109/+47) gadC/gadB glutamate:gamma‑aminobutyric acid antiporter/glutamate decarboxylase B, PLP‑dependent
?NC_000913_3_pae_tpiA 3666176 = 28 (0.700)intergenic (‑366/+4) gadX/gadA acid resistance regulon transcriptional activator; autoactivator/glutamate decarboxylase A, PLP‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGC  >  NC_000913_3_pae_tpiA/3666037‑3666316
                                                                                                                                           |                                                                                                                                            
gCTACATTGAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGgt                                                                                                                                     <  2:211098/149‑1 (MQ=255)
     aTTTAATAAACATTCATTTAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGtt                                                                                                                                <  1:75158/149‑1 (MQ=255)
      tttAATAAACATTCATATAACATATATCTTATCAACAAGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTATTGACGCTGTTTTTACAACGTTATGTTATCAGGTGTGttt                                                                                                                               <  2:654163/149‑1 (MQ=255)
         aataaACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTaaa                                                                                                                            <  2:319240/149‑1 (MQ=255)
                        aaCATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATACGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGc                                                                                                             >  2:85192/1‑149 (MQ=255)
                                  ttACCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGc                                                                                                   >  2:270007/1‑149 (MQ=255)
                                     tCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGt                                                                                                >  2:658144/1‑149 (MQ=255)
                                     tCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGAAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGt                                                                                                >  2:184258/1‑149 (MQ=255)
                                                       aGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTgaga                                                                              <  1:270007/149‑1 (MQ=255)
                                                                attattGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAgg                                                                     >  2:223303/1‑149 (MQ=255)
                                                                attattCCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGAATTTTACAACGTTATGTTAGCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAgg                                                                     <  2:184494/149‑1 (MQ=255)
                                                                    ttGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAgg                                                                 <  1:223303/149‑1 (MQ=255)
                                                                        gATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCttt                                                             <  1:109392/149‑1 (MQ=255)
                                                                             ataaGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCCGGGAGGCTTtataat                                                        >  2:706581/1‑147 (MQ=255)
                                                                               aaGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTACGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCt                                                      >  1:417256/1‑149 (MQ=255)
                                                                                 gCAACCGAATGCCCAGCTTTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTc                                                    <  1:498519/149‑1 (MQ=255)
                                                                                 gCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTACGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTc                                                    <  2:417256/149‑1 (MQ=255)
                                                                                   aaCCGAATGCCCTGATGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGTTATTTCAGGGAGGCTTTGTAGTCTTCCa                                                  <  2:226043/149‑1 (MQ=255)
                                                                                          tGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAg                                           >  1:34653/1‑149 (MQ=255)
                                                                                              cAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCa                                       >  1:59502/1‑149 (MQ=255)
                                                                                                               gcgtgtcATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTAGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGaa                      <  2:359635/143‑1 (MQ=255)
                                                                                                                cTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAg                     >  1:573472/1‑149 (MQ=255)
                                                                                                                   ggCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCATGCAGATACGGATGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTACAGCAACAGTTCAGCAAAGTCCCTTTCGAAGCcg                  >  1:23824/1‑149 (MQ=17)
                                                                                                                    gCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCcgc                 <  1:706581/149‑1 (MQ=255)
                                                                                                                      aTTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGcga               >  1:609695/1‑149 (MQ=255)
                                                                                                                       ttCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGcgac              <  1:184258/149‑1 (MQ=255)
                                                                                                                              tttACAACGTTATGTTATCCGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCATCGTAGTCTTACAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACCCAt       >  2:601202/1‑149 (MQ=14)
                                                                                                                                   aaCGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGATGGGCAATACCCTGCAGTTTCGGGTGATCGCAGAGATATTTAAGGGAGGCTCTGTAGTCATCCAGCAACAGTTCAGCAAAATCCATTTCGACGCAGCGGCGCCACATAATgc  >  2:215779/1‑149 (MQ=11)
                                                                                                                                           |                                                                                                                                            
GCTACATTTAATAAACATTCATATAACATATATCTTATCAACACGATGAATAGACAGCCAATATATTATTGCGATTAATAAGCAACCGAATGCCCAGCTGTTTTTTTAAAGGCTGGGCATTCGGTTTTTACAACGTTATGTTATCAGGTGTGTTTAAAGCTGTTCTGCTGGGCAATACCCTGCAGTTTCGGGTGATCGCTGAGATATTTCAGGGAGGCTTTGTAGTCTTCCAGCAACAGTTCAGCAAAGTCCATTTCGAAGCCGCGACGACACATAATGC  >  NC_000913_3_pae_tpiA/3666037‑3666316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.