Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_tpiA 4,158,703 A→G T100A (ACA→GCA)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_tpiA4,158,7030AG81.1% 399.3 / 76.8 175T100A (ACA→GCA) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (15/18);  new base G (72/70);  total (87/88)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGT  >  NC_000913_3_pae_tpiA/4158556‑4158851
                                                                                                                                                   |                                                                                                                                                    
aCCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCcgca                                                                                                                                                    <  3:1241568/150‑1 (MQ=255)
   aGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGtt                                                                                                                                                 <  3:658378/150‑1 (MQ=255)
    gCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTg                                                                                                                                                >  2:2601537/1‑150 (MQ=255)
       gTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGAc                                                                                                                                             <  1:234121/150‑1 (MQ=255)
         aaaGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCg                                                                                                                                           >  4:13740/1‑150 (MQ=255)
              gttgttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCt                                                                                                                                      <  1:2233569/150‑1 (MQ=255)
                  ttCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTa                                                                                                                                  >  4:767816/1‑150 (MQ=255)
                       ccAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCa                                                                                                                             <  2:890028/150‑1 (MQ=255)
                            cGGGAATGCTGCTGGTGGCTCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCAtatta                                                                                                                        >  3:956949/1‑150 (MQ=255)
                              ggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTAc                                                                                                                                        >  1:9374/1‑132 (MQ=255)
                              ggAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATc                                                                                                                      >  3:1344707/1‑150 (MQ=255)
                                aaTGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTg                                                                                                                                                <  2:1181801/122‑1 (MQ=255)
                                aaTGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTg                                                                                                                                                >  1:1181801/1‑122 (MQ=255)
                                   gctgctGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAt                                                                                                                 <  3:811056/150‑1 (MQ=255)
                                     tgctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgc                                                                                                               >  4:989653/1‑150 (MQ=255)
                                      gctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtg                                                                                                                <  1:718270/148‑1 (MQ=255)
                                       ctgGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                             >  1:146685/1‑150 (MQ=255)
                                          gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                          <  1:2601537/150‑1 (MQ=255)
                                          gtggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCAc                                                                                                          >  1:575031/1‑150 (MQ=255)
                                            ggATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCa                                                                                                        <  4:839727/150‑1 (MQ=255)
                                             gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCa                                                                                                                             >  2:2395894/1‑128 (MQ=255)
                                             gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                                                                                       >  3:100326/1‑150 (MQ=255)
                                             gATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAg                                                                                                       <  1:967778/150‑1 (MQ=255)
                                                  ggCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATcc                                                                                                                     >  2:162889/1‑131 (MQ=255)
                                                  ggCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCtt                                                                                                  >  1:833285/1‑150 (MQ=255)
                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGc                                                                                                                              >  1:2392563/1‑121 (MQ=255)
                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                                 >  1:392530/1‑150 (MQ=255)
                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGACAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                             <  4:724256/138‑1 (MQ=255)
                                                   gcgcGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGACAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                             >  3:724256/1‑138 (MQ=255)
                                                      cgTACCGTGCTGCTTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCa                                                                                              <  2:2325490/150‑1 (MQ=255)
                                                      cgTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCa                                                                                              <  4:100326/150‑1 (MQ=255)
                                                           cGTGCTGCGTGAGGTGCAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         <  3:13740/150‑1 (MQ=255)
                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         <  2:392530/150‑1 (MQ=255)
                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         <  1:2100382/150‑1 (MQ=255)
                                                           cGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGAATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         <  2:2130723/150‑1 (MQ=255)
                                                            gTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTg                                                                                        <  2:733120/150‑1 (MQ=255)
                                                               ctgcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCt                                                                                                                   <  1:1471654/120‑1 (MQ=255)
                                                                 gcGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGa                                                                                                      >  2:402404/1‑131 (MQ=255)
                                                                     gAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                               >  4:1566796/1‑150 (MQ=255)
                                                                      gggTGAAAGTCCTTAAAGAGATGGCCAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATc                                                                              <  2:1852701/149‑1 (MQ=255)
                                                                         tGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                               >  2:1709685/1‑146 (MQ=255)
                                                                         tGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGc                                                                           >  1:2274798/1‑150 (MQ=255)
                                                                           aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                         >  2:826995/1‑150 (MQ=255)
                                                                           aaaGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCAt                                                                         <  3:1566796/150‑1 (MQ=255)
                                                                                 cTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGca                                                                   <  3:996533/150‑1 (MQ=255)
                                                                                  ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                                 <  3:166346/119‑1 (MQ=255)
                                                                                  ttAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcac                                                                  <  4:1344707/150‑1 (MQ=255)
                                                                                   tAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGcaca                                                                 >  4:1075057/1‑150 (MQ=255)
                                                                                    aaaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAg                                                                <  4:484113/150‑1 (MQ=255)
                                                                                     aaGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGa                                                               <  2:575031/150‑1 (MQ=255)
                                                                                       gagaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAcc                                                             <  2:833285/150‑1 (MQ=255)
                                                                                       gagaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAcc                                                             >  2:576508/1‑150 (MQ=255)
                                                                                        agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctg                                                                                                             >  4:1166058/1‑101 (MQ=255)
                                                                                        agaTGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         >  1:2540216/1‑121 (MQ=255)
                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAcc                                                             >  2:1139811/1‑145 (MQ=255)
                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGAcc                                                             <  1:1139811/145‑1 (MQ=255)
                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccacca                                                        <  2:518130/150‑1 (MQ=255)
                                                                                            ggCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTAcc                                                                                                                                <  1:517361/78‑1 (MQ=255)
                                                                                             gCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAg                                                       >  1:473095/1‑150 (MQ=255)
                                                                                               aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTa                                                                                >  1:1337041/1‑123 (MQ=255)
                                                                                               aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTa                                                                                <  2:1337041/123‑1 (MQ=255)
                                                                                               aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                                 >  4:54640/1‑106 (MQ=255)
                                                                                               aaGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCttt                                                                                                 <  3:54640/106‑1 (MQ=255)
                                                                                                aGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTa                                                    >  4:421139/1‑150 (MQ=255)
                                                                                                  ccAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         >  2:149220/1‑111 (MQ=255)
                                                                                                  ccAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCt                                                                                         <  1:149220/111‑1 (MQ=255)
                                                                                                  ccAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGa                                                                       <  1:1083485/129‑1 (MQ=255)
                                                                                                    agcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATc                                                                                                                      >  2:2514844/1‑80 (MQ=255)
                                                                                                    agcagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgctgc                                                                                                            <  1:2410691/90‑1 (MQ=255)
                                                                                                      cagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGt                                                                                                                                          <  2:205109/58‑1 (MQ=255)
                                                                                                      cagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGt                                                                                                                                          >  1:205109/1‑58 (MQ=255)
                                                                                                      cagGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgcg                                              >  1:2463259/1‑150 (MQ=255)
                                                                                                       agGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGgcgc                                             <  2:502287/150‑1 (MQ=255)
                                                                                                        gggCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCa                                            <  2:635737/150‑1 (MQ=255)
                                                                                                          gCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAAc                                          >  2:2262664/1‑150 (MQ=255)
                                                                                                           cGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAGTGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACt                                         >  1:267347/1‑150 (MQ=255)
                                                                                                                 gATGTCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAg                                   >  4:118785/1‑150 (MQ=255)
                                                                                                                     tCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGc                               >  3:681081/1‑150 (MQ=255)
                                                                                                                     tCCGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGc                               <  2:146685/150‑1 (MQ=255)
                                                                                                                     tCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGc                               <  2:2304942/150‑1 (MQ=255)
                                                                                                                      ccGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTAt                                                                               >  1:1767013/1‑101 (MQ=255)
                                                                                                                       cGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACccac                                                          >  4:305837/1‑121 (MQ=255)
                                                                                                                       cGGACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaa                             >  1:2146591/1‑150 (MQ=255)
                                                                                                                        ggACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCaaa                            <  3:1075057/150‑1 (MQ=255)
                                                                                                                         gACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgct                                                                                                              >  3:118125/1‑67 (MQ=255)
                                                                                                                         gACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTAtgct                                                                                                              <  4:118125/67‑1 (MQ=255)
                                                                                                                         gACCGCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAAc                           >  2:948255/1‑150 (MQ=255)
                                                                                                                             gCTGCACATTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGa                       <  3:1352616/150‑1 (MQ=255)
                                                                                                                                 cacaTTGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTg                                                                                        <  1:1659585/81‑1 (MQ=255)
                                                                                                                                     ttGGTTTGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCGGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGa               >  1:1002427/1‑150 (MQ=255)
                                                                                                                                       ggTTTGCTGCCCGCCGTTGGACGCTACCTGCTACGGCATATTCTCCCTATGCTGCACCAGACCTTTCCAAAGCTGGATGTGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATc             <  4:956949/150‑1 (MQ=255)
                                                                                                                                       ggTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTa                                                                                <  2:588717/83‑1 (MQ=255)
                                                                                                                                       ggTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTa                                                                                >  1:588717/1‑83 (MQ=255)
                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                     <  1:451780/92‑1 (MQ=255)
                                                                                                                                         tttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAg                                                                     >  2:451780/1‑92 (MQ=255)
                                                                                                                                          ttGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTc          <  2:2146591/150‑1 (MQ=255)
                                                                                                                                           tGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcg         >  2:1343871/1‑150 (MQ=255)
                                                                                                                                           tGATTCCCGCAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcg         >  4:673050/1‑150 (MQ=255)
                                                                                                                                                   acaGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcg         <  4:1050719/142‑1 (MQ=255)
                                                                                                                                                   acaGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTcg         >  3:1050719/1‑142 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACCAGCCGTAAAGTGTTGTTCACCCAGGCGGGAATGCTGCTGGTGGATCAGGCGCGTACCGTGCTGCGTGAGGTGAAAGTCCTTAAAGAGATGGCAAGCCAGCAGGGCGAGACGATGTCCGGACCGCTGCACATTGGTTTGATTCCCACAGTTGGACCGTACCTGCTACCGCATATTATCCCTATGCTGCACCAGACCTTTCCAAAGCTGGAAATGTATCTGCATGAAGCACAGACCCACCAGTTACTGGCGCAACTGGACAGCGGCAAACTCGATTGCGTGATCCTCGCGCTGGT  >  NC_000913_3_pae_tpiA/4158556‑4158851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: